李慧;罗正荣;张青林;

• 1:华中农业大学园艺植物生物学教育部重点实验室

摘要(Abstract)：

【目的】‘关口葡萄’是湖北建始县的"地理标志产品",但其品种来源和分类学地位有待明确。【方法】以‘关口葡萄’为试材,以其他14份葡萄种质包括5份欧亚种(Vitis vinifera L.)、6份欧美杂交种(V.vinifera×V.labrusca)以及3份美洲种(V.labrusca)为对照;采用SSR和IRAP分子标记技术,通过对供试材料间亲缘关系的分析而鉴定其身份。【结果】利用筛选出的16对SSR引物以及10条IRAP引物对供试材料基因组DNA的扩增结果表明,两种标记共扩增271条清晰、可重复谱带,其中多态性带264条,多态性比例分别为99.41%和94.12%;应用NTSYS-pc version2.10e软件进行聚类分析,两种标记的树状图结果均显示‘关口葡萄’与欧美杂交种‘尼加拉’和‘白香蕉’亲缘关系最近,与美洲种‘康可’和‘郑果6号’亲缘关系较近,而与其他欧亚种品种的遗传关系相对较远。【结论】‘关口葡萄’属欧美杂交种,其既非‘尼加拉’也非‘白香蕉’,但其来源可能与二者有关。

关键词(KeyWords)： ‘关口葡萄’;SSR;IRAP;身份鉴定;亲缘关系

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础性工作专项(2012FY110100)

作者(Author): 李慧;罗正荣;张青林;

Email:

参考文献(References)：

• [1]LIANG Yun-fu,HU Fu-xian,HUANG Ben-rong.The development status and countermeasure of fruit industry in Jianshi county[J].Hubei Forestry Science,2012,177(5):65-67.梁运甫,胡富宪,黄本荣.建始县林果产业发展现状与对策[J].湖北林业科技,2012,177(5):65-67.
• [2]SUN Zhi-guo,XIONG Wan-zhen,WANG Shu-ting,ZHONG Xue-bin.Intellectual property protection of geographical indications of the traditional specialty in Hubei province[J].Journal of Anhui Agricultural Science,2012,40(4):2407-2410.孙志国,熊晚珍,王树婷,钟学斌.湖北传统特产的地理标志知识产权保护对策[J].安徽农业科学,2012,40(4):2407-2410.
• [3]CHEN Jie-zhong.Fruit culture in South China(Third Edition)[M].Beijing:China Agricultural Press,2003:420-421.陈杰忠.果树栽培学各论(南方本)[M].3版.北京:中国农业出版社,2003:420-421.
• [4]NATAA,LIDIJA T,DRAGOSLAV I,NADA K,TATJANA C J,KLIME B,ELIZABETA A,VESNA M,BRANKA J.Microsatellite inferred genetic diversity and structure of Western Balkan grapevines(Vitis vinifera L.)[J].Tree Genetic&Genomes,2014,10(1):127-140.
• [5]MA Li-li,XIE Kai-dong,YE Jun-li,GUO Wen-wu.Phylogenetic relationship for early flowering lemon by SSR markers[J].Journal of Fruit Science,2013,30(3):381-385.马丽丽,解凯东,叶俊丽,郭文武.基于SSR标记的早花柠檬亲缘关系分析[J].果树学报,2013,30(3):381-385.
• [6]SUN Ping,ZONG Yu,LIU Jing,HU Chun-yun,TENG Yuanwen.Study on genetic diversity of Malus sieboldii in Qingliangfeng Region based on SSR markers[J].Journal of Fruit Science,2013,30(1):8-15.孙萍,宗宇,刘晶,胡春云,滕元文.基于SSR标记的清凉峰地区三叶海棠遗传多样性研究[J].果树学报,2013,30(1):8-15.
• [7]DU Xiao-yun,ZHANG Qing-lin,LUO Zheng-rong.Development of retrotransposon primers and their utilization for germplasm identification in Diospyros spp.(Ebenaceae)[J].Tree Genetics&Genomes,2009,5(1):235-245.
• [8]DU Xiao-yun,HU Qian-ni,ZHANG Qing-lin,WANG Yanbo,LUO Zheng-rong.Transferability of retrotransposon primersderived from Persimmon(Diospyros kaki Thunb.)across other plant species[J].Genetics and Molecular Research,2013,12(2):1781-1795.
• [9]TIAN Zhi-shuo,FAN Xiu-cai,JIANG Jian-fu,SUN Haisheng,ZHANG Guo-hai,LIU Chong-huai.A introduction of the aliases to common grape varieties[J].Northern Horticulture,2012(5):188-190.田智硕,樊秀彩,姜建福,孙海生,张国海,刘崇怀.常见葡萄品种别名介绍[J].北方园艺,2012(5):188-190.
• [10]LI Jin-lu,WANG Shuo,YU Jing,WANG Ling,ZHOU Shi-liang.A modified CTAB protocol for plant DNA extraction[J].Chinese Bulletin of Botany,2013,48(1):72-78.李金璐,王硕,于婧,王玲,周世良.一种改良的植物DNA提取方法[J].植物学报,2013,48(1):72-78.
• [11]BASSAM B J,CAETANO-ANOLLéS G,GRESSHOFF M P.Fast and sensitive silver staining of DNA in polyacrylamide gels[J].Analytical Biochemistry,1991,196(1):80-83.
• [12]THOMAS M R,SCOTT N S.Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites(STSs)[J].Theoretical and Applied Genetics,1993,86(8):985-990.
• [13]BOWERS J E,DANGL G S,VIGNANI R,MEREDITH C P.Isolation and characterization of new polymorphic simple sequence repeat loci in grape(Vitis vinifera L.)[J].Genome,1996,39(4):628-633.
• [14]ARROYO-GARCíA R,MARTíNEZ-ZAPATER J M.Development and characterization of new microsatellite markers for grape[J].Vitis,2004,43(4):175-178.
• [15]BLASI P,BLANC S,WIEDEMANN M S,PRADO E,ERNST H R,MESTRE P,MERDINOGLU D.Construction of a reference linkage map of Vitis amurensis and genetic mapping of Rpv8,a locus conferring resistance to grapevine downy mildew[J].Theoretical and Applied Genetics,2011,123(1):43-53.
• [16]DU Xiao-yun,ZHANG Qing-lin,LI Bao,LENG Ping,ZHANG Ming-de,HUANG Jian-min,LUO Zheng-rong.Mutations identification based on IRAP analysis in Diospyros kaki cv.Damopan[J].Journal of Fruit Science,2011,28(2):257-262.杜晓云,张青林,李宝,冷平,张明德,黄建民,罗正荣.基于IRAP技术的磨盘柿变异单株鉴定[J].果树学报,2011,28(2):257-262.
• [17]ROHLF F J.NTSYS-PC,Numerical taxonomy and multivariate analysis system Version 2.10e[M].Exeter Software,Setauket,NewYork,USA.2000.
• [18]POWELL W,MORGANTE M,ANDRE C,HANAFEY M,VOGEL J,TINGEY S,RAFALSKI J A.The comparison of RFLP,RAPD,AFLP and SSR(microsatellite)markers for germplasm analysis[J].Molecular Breeding,1996,2(3):225-238.
• [19]LIU Zhen-dong.High density molecular genetic map construction in Vitis amurensis Rupr.and QTL location for grape cold hardiness[D].Shenyang:Shenyang Agricultural University,2012.刘镇东.山葡萄高密度分子遗传图谱构建及抗寒性QTL定位研究[D].沈阳:沈阳农业大学,2012.
• [20]BOWERS J,BOURSIQUOT J M,THIS P,CHU K,JOHANSSON H,MEREDITH C.Historical genetics:the parentage of chardonnay,gamay,and other wine grapes of northeastern France[J].Science,1999,285(5433):1562-1565.
• [21]YUTAKA S,TOSHIHIRO S,NORIO T TOSHIYA Y,TETSUYA K,TATEKI H,KAZUO K.Identification of parentage of Japanese pear‘Housui’[J].Journal of the Japanese Society for Horticultural Science,2004,73(6):511-518.
• [22]KALENDAR R,GROB T,REGINA M,SUONIEMI A,SCHULMAN A.IRAP and REMAP:two new retrotransposon-based DNA fingerprinting techniques[J].Theoretical and Applied Genetics,1999,98(5):704-711.
• [23]D’ONOFRIO C,LORENZIS G D,GIORDANI T,NATALI L,CAVALLINI A,SCALABRELLI G.Retrotransposon-based molecular markers for grapevine species and cultivars identification[J].Tree Genetics&Genomes,2010,6(3):451-466.
• [24]SHEN Y Y,DING X J,WANG W,CAI B H,GAO Z H,ZHANG Z.Analysis of genetic diversity in Japanese apricot(Prunus mume Sieb.et Zucc.)based on REMAP and IRAP molecular markers[J].Scientia Horticulturae,2011,132:50-58.
• [25]WANG Wen-yan,WANG Chen,TAO Jian-min,YANG Guang,FANG Jing-gui.Situation of nomenclature of introduced foreign grapevine varieties in China[J].Acta Agriculturae Jiangxi,2010,22(11):40-44.王文艳,王晨,陶建敏,杨光,房经贵.中国外来引进葡萄品种命名情况分析[J].江西农业学报,2010,22(11):40-44.
• [26]QIAN Chun.Studies on identifying and tetraploid inducing in bud mutation of‘White Banana’grape[D].Chongqing:Southwest University,2006.钱春.白香蕉葡萄芽变突变体鉴定及四倍体诱导研究[D].重庆:西南大学,2006.