李慧;罗正荣;张青林;

• 1:华中农业大学园艺植物生物学教育部重点实验室

摘要(Abstract)：

【目的】‘关口葡萄’是湖北建始县的"地理标志产品",但其品种来源和分类学地位有待明确。【方法】以‘关口葡萄’为试材,以其他14份葡萄种质包括5份欧亚种(Vitis vinifera L.)、6份欧美杂交种(V.vinifera×V.labrusca)以及3份美洲种(V.labrusca)为对照;采用SSR和IRAP分子标记技术,通过对供试材料间亲缘关系的分析而鉴定其身份。【结果】利用筛选出的16对SSR引物以及10条IRAP引物对供试材料基因组DNA的扩增结果表明,两种标记共扩增271条清晰、可重复谱带,其中多态性带264条,多态性比例分别为99.41%和94.12%;应用NTSYS-pc version2.10e软件进行聚类分析,两种标记的树状图结果均显示‘关口葡萄’与欧美杂交种‘尼加拉’和‘白香蕉’亲缘关系最近,与美洲种‘康可’和‘郑果6号’亲缘关系较近,而与其他欧亚种品种的遗传关系相对较远。【结论】‘关口葡萄’属欧美杂交种,其既非‘尼加拉’也非‘白香蕉’,但其来源可能与二者有关。

关键词(KeyWords)： ‘关口葡萄’;SSR;IRAP;身份鉴定;亲缘关系

Abstract:

Keywords:

基金项目(Foundation): 国家科技基础性工作专项(2012FY110100)

作者(Author): 李慧;罗正荣;张青林;

Email:

DOI: 10.13925/j.cnki.gsxb.20140202

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