张政达;王景荣;陈丽辉;樊佳茹;韩晓云;王革伏;张志忠;

• 1:福建农林大学园艺学院·福建农林大学-戴尔豪西大学联合实验室
• 2:戴尔豪西大学农学院

摘要(Abstract)：

【目的】克隆根系分泌物介导的甜瓜自毒胁迫响应基因Fe-SOD,分析基因序列特征和表达情况,探讨其功能。【方法】以甜瓜幼苗为材料,通过RT-PCR技术克隆Fe-SOD基因,命名为CmFe-SOD,进行生物信息学分析和亚细胞定位;通过qRT-PCR技术分析CmFe-SOD在自毒胁迫后甜瓜幼苗叶片和根系中的表达模式。【结果】CmFe-SOD基因组DNA全长1 255 bp,开放阅读框918 bp,编码305个氨基酸。生物信息学分析显示CmFe-SOD为酸性亲水蛋白,无信号肽和跨膜区域,二级结构中无规则卷曲比例最高,与三级结构预测结果一致,基因序列包含典型的Fe-SOD蛋白结构域。同源性分析表明,不同来源Fe-SOD在氨基酸序列上具有较高同源性,CmFe-SOD蛋白与黄瓜的关系较近。通过洋葱内表皮GFP荧光定位观察发现,CmFe-SOD定位于细胞质;qRT-PCR结果显示,根系分泌物处理后甜瓜幼苗叶片中CmFe-SOD基因表达水平整体呈增加趋势,在24 h时表达量最高;在根系中呈现先增加后下降趋势,6 h时表达量最高。【结论】CmFe-SOD基因在进化上较保守,参与了甜瓜根系分泌物介导的自毒胁迫响应,自毒胁迫后在叶片中表达量呈持续上升的趋势,在根部呈现先上升后下降的趋势。

关键词(KeyWords)： 甜瓜;自毒胁迫;根系分泌物;Fe-SOD基因;亚细胞定位;qRT-PCR

Abstract:

Keywords:

基金项目(Foundation): 国家重大农技推广服务试点项目(KNJ-152046);; 福建农林大学创新专项基金(CXZX2016108,CXZX2017168);福建农林大学园艺学院青年学术骨干培养基金

作者(Author): 张政达;王景荣;陈丽辉;樊佳茹;韩晓云;王革伏;张志忠;

Email:

DOI: 10.13925/j.cnki.gsxb.20180132

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