不同发育阶段甜樱桃花授粉后花粉萌发和花粉管生长的差异Discrepancy of pollen germination and pollen tube growth at different mature degree of pistil in sweet cherry
李晓;吴华清;吴俊;陶书田;张绍铃;
摘要(Abstract):
选用自交不亲和性程度不同的2个甜樱桃品种莫利和拉宾斯为试材,分别在开花前、开花当天和开花后进行莫利自花、拉宾斯自花和莫利×拉宾斯异花授粉,并于授粉后12、24、48、72、96h切取花柱,用FAA固定,荧光染色后压片观察。结果表明,3个组合的花粉萌发率都以开花当天授粉的最佳,拉宾斯自交和莫利×拉宾斯的花粉管生长状况也以开花当天授粉的最佳,但莫利自交的花粉管生长状况却以花前第4天最佳,花后第4天次之,而当天授粉的最差。因此认为,甜樱桃自交不亲和程度与其雌蕊的发育状态密切相关,蕾期授粉和延迟授粉都能够在一定程度上克服甜樱桃的自交不亲和性,但蕾期授粉的作用更加明显。
关键词(KeyWords): 甜樱桃;自交不亲和;蕾期授粉;延迟授粉
基金项目(Foundation): 高校博士学科点专项科研基金(NO.B200523)
作者(Author): 李晓;吴华清;吴俊;陶书田;张绍铃;
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