朱自果;王跃进;史江莉;王沛雅;张朝红;

• 1:农业部西北园艺植物种质资源利用重点开放实验室
• 2:陕西省农业分子生物学重点实验室
• 3:西北农林科技大学园艺学院

摘要(Abstract)：

以抗白粉病的华东葡萄杂种后代6-12-4株系为试材,利用SMARTTM技术构建了白粉病病原菌诱导的全长cDNA文库。经检测,原始文库滴度为2.50×106pfu·mL-1,重组率达到99%,扩增文库滴度为3.0×109pfu·mL-1,重组率达到95%,插入片段长度在500～2000bp。随机挑取300个克隆,测序获得260条质量较高的ESTs,聚类及拼接后,共得到183个一致序列(所获序列已经提交GenBank,登录号为FG579859-FG580039)。经蛋白功能预测分析,获得含有TPR结构域的小的富含谷氨酰胺的蛋白、MYB类转录因子、水通道蛋白、富含脯氨酸蛋白、亲环素蛋白、ACI14蛋白、转脂蛋白等抗病相关基因,涉及到植物的信号转导,胁迫诱导,防卫反应等方面。这些EST序列为利用中国野生华东葡萄进行抗白粉病育种研究和利用提供了有价值的抗病基因片段,为丰富世界葡萄属植物抗白粉病基因提供序列数据。

关键词(KeyWords)： 葡萄;cDNA文库;白粉病;EST

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30571280&No.30771493);; 农业部公益性行业科研专项200803038

作者(Author): 朱自果;王跃进;史江莉;王沛雅;张朝红;

Email:

DOI:

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