宋尚伟;张恒涛;张芳明;王娟;陶永焕;

• 1:河南农业大学园艺学院
• 2:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】开发苹果EST-SSR引物,评价苹果种质资源的多样性与亲缘关系。【方法】利用NCBI数据库中苹果EST的SSR位点开发了35对EST-SSR引物,建立了苹果EST-SSR体系,并利用筛选出的16对引物对苹果品种资源的亲缘关系进行研究。【结果】118份基因组DNA共扩增出等位基因位点138个,位点数的变化从3到13不等,平均每对引物检测到8.62个位点,总的多态性比率为94.2%,各引物的多态性比例分布为81.8%～100%,相似系数为0.48～1.00。利用UPGMA法构建聚类树状图,在相似性系数0.65处可将118个供试材料分为5大组:其中第1组又可分为2个亚组,包含了绝大部分供试材料;第2、3、4和5组包含的品种数目分别为12个、11个、3个和2个;第5组与其他组亲缘关系较远,相似性系数仅为0.48。【结论】筛选出的16对EST-SSR引物可用于评价苹果种质资源间的遗传多样性。

关键词(KeyWords)： 苹果;EST-SSR引物;亲缘关系;聚类分析;相似性系数

Abstract:

Keywords:

基金项目(Foundation): 河南省教育厅科学技术研究重点项目(13A210484)

作者(Author): 宋尚伟;张恒涛;张芳明;王娟;陶永焕;

Email:

DOI: 10.13925/j.cnki.gsxb.2013.04.009

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