苹果锈果类病毒新疆分离物的克隆和序列分析Cloning and sequencing of Sinkiang isolate of apple scar skin viroid (ASSVd)
赵英;牛建新;
摘要(Abstract)：
利用自行设计的方法分离提取总RNA,通过一对特异引物,扩增出苹果锈果类病毒的全长片段,该片段通过回收、克隆和测序,结果发现该片段全长330bp,与已发表的NC_001340同源性为99.7%,仅在第126有一个碱基差异。由此证明该片段是苹果锈果类病毒的全长序列,进一步证明该反应体系能很好地用于苹果锈果类病毒的RT-PCR检测,为快速鉴定类病毒奠定了良好基础。
关键词(KeyWords)： 苹果锈果类病毒;RT-PCR;基因组;测序
基金项目(Foundation): 国家自然科学基金资助项目(30360066);; 国家科技攻关计划引导项目(2003BA546C);; 兵团科委项目(NKB02SDXNK01SW)。
作者(Author): 赵英;牛建新;
Email:
参考文献(References)：
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