苹果锈果类病毒新疆分离物的克隆和序列分析Cloning and sequencing of Sinkiang isolate of apple scar skin viroid (ASSVd)
赵英;牛建新;
摘要(Abstract)：
利用自行设计的方法分离提取总RNA,通过一对特异引物,扩增出苹果锈果类病毒的全长片段,该片段通过回收、克隆和测序,结果发现该片段全长330bp,与已发表的NC_001340同源性为99.7%,仅在第126有一个碱基差异。由此证明该片段是苹果锈果类病毒的全长序列,进一步证明该反应体系能很好地用于苹果锈果类病毒的RT-PCR检测,为快速鉴定类病毒奠定了良好基础。
关键词(KeyWords)： 苹果锈果类病毒;RT-PCR;基因组;测序
基金项目(Foundation): 国家自然科学基金资助项目(30360066);; 国家科技攻关计划引导项目(2003BA546C);; 兵团科委项目(NKB02SDXNK01SW)。
作者(Author): 赵英;牛建新;
Email:
DOI:
参考文献(References)：
- [1]YANG X,HADIDI A,HAMMOND R W.Nucleotide sequence of apple scar skin viroid reverse transcribed in host extracts and amplified by the polymerase chain reaction[J].Acta Horticulturae,1992,309:305-309.
- [2]HASHIMOTO J,KOGANEZAWA H.Nucleotide sequence and secondary structure of apple scar skin viroid[J].Nucleic Acids Research,1987,15(17):7045-7052.
- [3]HADIDI A,YANG X.Detection of pome fruit viroids by enzymatic cDNA amplification[J].J Virol Methods,1990,30:261-270.
- [4]HADIDI A,HANSEN A J,PARISH C J,et al.Scar skin and dapple apple viroids are seed-borne and persistent in infected apple trees[J].Res Virol,1991,142:289-296.
- [5]MA B G(马兵钢).Molecular detection of main Korla Pear viruses in xinjiang(新疆库尔勒香梨主要病毒的分子检测研究)[D].South China University of Tropical Agriculture,2004.(in Chinese)
- [6]PUCHTA H,LUCKINGER R,YANG X C,et al.Nucleotide sequence and secondary structure of apple scar viroid(ASSVd)from China[J].Plant Molecular Biology,1990,14(6):1065-1067.
- [7]ZHU S F,HADIDI A,HAMMOND R W.Nucleotide sequence and secondary structure of pome fruit viroids from apple disease apples,pear rusty skin diseased pears and apple scar skin symptomless pear[J].Acta Horticulturae,1995,386:554-559.