张慧;黄新忠;傅敏;洪霓;王国平;

• 1:华中农业大学植物科学技术学院·湖北省作物病害监测与安全控制重点实验室
• 2:福建省农业科学院果树研究所

摘要(Abstract)：

【目的】明确近年在福建砂梨产区新发生的梨急性花枯病的病原菌种类。【方法】从产区采集显现急性花枯症状的‘翠冠’梨病样品进行组织分离,并利用形态学和分子生物学相结合的方法对获得的菌株进行种类鉴定和致病性验证。【结果】通过组织分离和纯化,并根据其菌落形态特征从福建砂梨急性花枯病样品中获得31个刺盘孢属(Colletotrichum)菌株。选取其中的14个代表菌株进行形态学观察和多基因(ITS、ACT、TUB、CHS-1及GAPDH)系统发育分析的结果显示,它们均为松针刺盘孢(C. fioriniae)。在一周花龄的砂梨(品种‘丰水梨’)、西洋梨(品种‘三季梨’)和秋子梨(品种‘南果梨’)的离体花序上喷雾接种代表菌株(CFZH1、CFZH6、CFZH15)分生孢子悬浮液的结果显示,均产生有急性花枯症状,平均发病率分别95.8%、63.9%和81.3%。【结论】福建砂梨产区导致梨急性花枯病的病原菌为松针刺盘孢(C. fioriniae)。本研究是福建砂梨产区松针刺盘孢引起梨花枯死的首次报道。

关键词(KeyWords)： 砂梨;福建;急性花枯;松针刺盘孢

Abstract:

Keywords:

基金项目(Foundation): 国家重点研发项目（2018YFD0201406）;; 国家梨产业技术体系（CARS-28-16）

作者(Author): 张慧;黄新忠;傅敏;洪霓;王国平;

Email:

DOI: 10.13925/j.cnki.gsxb.20200029

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