肖龙;张彩霞;宗泽冉;田义;张利义;丛佩华;

• 1:农业部园艺作物种质资源利用重点实验室
• 2:中国农业科学院果树研究所

摘要(Abstract)：

【目的】开展苹果叶片应答轮纹病菌胁迫的叶绿体蛋白质组学研究,筛选苹果叶片应答轮纹病胁迫后差异表达的叶绿体蛋白。【方法】以未受轮纹病菌胁迫和受轮纹病胁迫的苹果叶片为试材,分别提取叶片叶绿体及其蛋白,利用双向电泳技术(2-DE)结合质谱技术(MALDI-TOF-TOF/MS)检测并分析差异表达蛋白点。【结果】叶绿体蛋白样品经双向电泳分离后,获得了背景清晰的双向电泳凝胶图谱。经质谱检测及数据库检索,成功鉴定得到21个差异表达蛋白点,按照功能划分为6类,分别为光合作用相关、能量代谢相关、蛋白代谢相关、氨基酸合成相关、抗氧化相关及未知功能蛋白。【结论】苹果叶片受轮纹病菌侵染后,叶绿体光合作用、能量代谢等多个代谢过程中的相关蛋白差异表达,应答轮纹病菌的胁迫。

关键词(KeyWords)： 苹果叶片;叶绿体蛋白;轮纹病菌;胁迫;差异蛋白质组;双向电泳

Abstract:

Keywords:

基金项目(Foundation): 农业部现代农业产业技术体系建设专项资金(CARS-28);; 国家科技支撑计划项目(2013BAD02B01)

作者(Author): 肖龙;张彩霞;宗泽冉;田义;张利义;丛佩华;

Email:

DOI:

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