红宝石无核葡萄胚珠培养成苗技术研究Technology of ovule culture for Ruby Seedless grape cultivar
赵密珍,苏家乐,钱亚明,王壮伟,刁曼妮
摘要(Abstract)：
以自然授粉的红宝石无核为试材,观察了胚珠生长状况,对胚珠离体培养与成苗技术进行了探索,结果表 明:红宝石无核的胚珠发育在花后35 d是其胚挽救的最佳时期。此时,取出胚珠接种在Nitsch+IAA 2.0 mg/L+GA 0.4 mg/L,附加水解酪蛋白100mg/L,活性炭2g/L的培养基中培养120 d,胚发育率为58％,后将胚珠切开处理并转接 到胚萌发培养基1/2MS+6-BA 0.5 mg/L中萌发,萌发率为16％,在1/2MS+6-IBA 0.2mg/L培养基上成苗,小苗生长 最好。通过前期保湿等措施,小苗移栽成活率达100％。
关键词(KeyWords)： 红宝石无核葡萄;胚珠培养;成苗技术
基金项目(Foundation): 南京市国际合作项目(2003ZB0101) 江苏省农业科技攻关项目(BE2001324-3)
作者(Author): 赵密珍,苏家乐,钱亚明,王壮伟,刁曼妮
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