红宝石无核葡萄胚珠培养成苗技术研究Technology of ovule culture for Ruby Seedless grape cultivar
赵密珍,苏家乐,钱亚明,王壮伟,刁曼妮
摘要(Abstract)：
以自然授粉的红宝石无核为试材,观察了胚珠生长状况,对胚珠离体培养与成苗技术进行了探索,结果表 明:红宝石无核的胚珠发育在花后35 d是其胚挽救的最佳时期。此时,取出胚珠接种在Nitsch+IAA 2.0 mg/L+GA 0.4 mg/L,附加水解酪蛋白100mg/L,活性炭2g/L的培养基中培养120 d,胚发育率为58％,后将胚珠切开处理并转接 到胚萌发培养基1/2MS+6-BA 0.5 mg/L中萌发,萌发率为16％,在1/2MS+6-IBA 0.2mg/L培养基上成苗,小苗生长 最好。通过前期保湿等措施,小苗移栽成活率达100％。
关键词(KeyWords)： 红宝石无核葡萄;胚珠培养;成苗技术
基金项目(Foundation): 南京市国际合作项目(2003ZB0101) 江苏省农业科技攻关项目(BE2001324-3)
作者(Author): 赵密珍,苏家乐,钱亚明,王壮伟,刁曼妮
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参考文献(References)：
- [1] 贺玮,孙山.美国阿肯色州的无核鲜食葡萄育种及育成品种[J]. 中国南方果树,2002,31(3):74-75.
- [2] Ramming DW. The use of embryo culture in fruit breeding[J]. HortScience, 1990,25(4) :393-398.
- [3] Mullins MG. Tissue culture and the genetic improvement of grapevines: a review[J]. Acta Horticulturae, 1990, (280): 11 -22.
- [4] Cain DW, Emershad RL,Tarailo RE. In ovulo embryo culture and seedling development of seed and seedless grapes (Vitis vinifera L.) [J].Vitis,1983,22: 9-14.
- [5] Emershad RL, Ramming DW,Serpe MD. In ovulo embryo development and plant formation from stenospermic genotypes of Vitis vinifera L.[J]. American Journal of Botany, 1989,76: 397-402.
- [6] Spiegel-Roy P,Sahar N,Baron J ,et al. In vitro culture and plant formation from grape cultivars with abortive ovules and seeds[J]. J Amer Soc Hort Sci, 1985,110:109-112.
- [7] Perl A, Saad S, Sahar N, et al. Establishment of long term embryo genic cultures of seedless Vitis vinifera cultivars-a synergistic effect of auxins and the role of abscisic acid[J]. Plant Sci, 1995,104:193-200.
- [8]张宏明,孟新法.无核葡萄胚珠发育及早期离体培养的研究Ⅰ激 素对胚珠离体培养的研究[J].北京农业大学学报,1990,16(2): 272-282.
- [9]董晓玲,贺普超.无核葡萄杨格尔的胚发育及胚珠培养[J].果树科 学,1991,8(2):87-90.
- [10]陈谦.葡萄推广新品种图谱[M].济南:山东科技出版社,2002.1.