于晓丽;亓超;王培松;李宝燕;王英姿;

• 1:烟台市农业科学研究院
• 2:山东省黄河三角洲高效生态农业发展研究院

摘要(Abstract)：

【目的】分离获得对果树真菌病害[苹果轮纹病(Botryospuaeria dothidea)、苹果斑点落叶病(Alternaria alternaria)、葡萄炭疽病(Colletotrichum gloeosporioides)、葡萄白腐病(Coniella diplodiella)和葡萄霜霉病(Plasmopara viticola)]具有明显拮抗作用的菌株,初步研究其作用机制,为果树真菌病害的生物防治提供潜在的材料菌株。【方法】从不同植物上分离内生菌,采用平板对峙法、菌落生长速率法及叶盘法等试验筛选拮抗菌;根据菌株的形态、生理生化特征、16S rDNA序列分析、gyrA基因序列分析等对其进行鉴定;通过测定拮抗菌发酵液对温度、pH值的敏感度分析拮抗物质的稳定性;通过测定拮抗菌发酵液对菌丝的影响,初步分析其拮抗机制。【结果】从不同植物根、茎、叶中共分离得到58株细菌,其中编号为YTB1407的细菌菌株对上述5种植物病原真菌有较好的拮抗作用,经鉴定该菌株为解淀粉芽孢杆菌,且该菌株发酵液的拮抗活性在温度为20~100℃、pH为2.0~10.0,比较稳定。经该菌株发酵液处理后的病原菌菌丝畸形。【结论】该拮抗菌株为Bacillus amyloliquefaciens;温度和pH值对该菌株拮抗物质活性影响不大;该拮抗菌株可以通过影响病原菌菌丝正常生长发挥其拮抗作用。

关键词(KeyWords)： 果树;真菌病害;拮抗细菌;鉴定;稳定性;作用机制

Abstract:

Keywords:

基金项目(Foundation): 山东省农业科学院院地科技合作引导计划(2015YDHZ15);; 公益性行业(农业)科研专项(201203035);; 山东省现代农业产业技术体系水果创新团队岗位专家(SDAIT-03-021-09);; 山东省中青年科学家科研奖励基金(博士基金)(BS2015NY004,BS2013NY016)

作者(Author): 于晓丽;亓超;王培松;李宝燕;王英姿;

Email:

DOI: 10.13925/j.cnki.gsxb.20150483

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