砂梨上苹果茎痘病毒的调查分析及RT-PCR与TC-RT-PCR检测研究Investigation and detection of Apple stem pitting virus in Pyrus pyrifolia by RT-PCR and TC-RT-PCR
侯雨萱,洪霓,邓晓云,胡红菊,王国平
摘要(Abstract)：
试验通过西方烟(Nicotianaoccidentalis‘37B’)和杂种(Pyroniaveitchii)、A20接种鉴定及PAS-ELISA检测,对保存于国家砂梨种质资源圃的38份砂梨样品进行了苹果茎痘病毒(Applestempittingvirus,ASPV)带毒率的调查分折。结果表明,生物学鉴定与血清学检测结果一致,ASPV带毒率均为26.3%。应用以dsRNA为模板RT-PCR检测该病毒,在杂种和A20上显症的6个样品均获得316bp目标片段。应用TC-RT-PCR检测显症的西方烟,也获得了预期的目标片段。
关键词(KeyWords)： 苹果茎痘病毒;生物学鉴定;PAS-ELISA;dsRNA;RT-PCR;TC-RT-PCR
基金项目(Foundation): 科技部863课题(项目编号:2001AA241142)。
作者(Author): 侯雨萱,洪霓,邓晓云,胡红菊,王国平
Email:
参考文献(References)：
- [1]G ella R.Effectofsom e virus diseases on the perform ance oftwo clones ofA gua de A ranjuez pear[J].A cta H orticulturae,1990,256:137-142.
- [2]王国平,洪霓,张尊平,等.我国北方梨产区主要品种病毒种类的鉴定研究[J].中国果树.1994,2:1-4.
- [3]牛建新,刘连科,王小兵,等.库尔勒香梨苹果茎痘病毒R T-PCR检测技术研究[J].中国农业科学,2003,36(5):561-566.
- [4]Jelkm ann W.N ucleotide sequence ofapple stem pitting virus and of the coatprotein gene ofa sim ilarvirusfrom pearassociated with vein yellows disease and their relationship with potex-and carlaviruses[J].JournalofG eneralV irology,1994,75:1535-1542.
- [5]洪霓,刘福昌,王国平,等.A蛋白酶联法检测苹果叶斑病毒和茎沟病毒研究[J].中国果树,1992,(1):44-47.
- [6]Jelkm ann W,Kunze L,V etten H J,etal.cD N A cloning ofdsRN A associated with apple stem pitting disease and evidence forthe rela-tionship ofthe virus-like agents associated with apple stem pitting and pearvein yellows[J].A cta H orticulturae,1992,309:55-62.
- [7]H addiA,D avis I,M inafra A,etal.A virus affecting apples and pearsisrelated to grapevine virusA asrevealed by IC-R T-PCR and m olecularhybridization[J].A cta H orticulturae,1995,386:37-43.
- [8]K oganezawa H,Y anase H.A new type ofelongated virusisolated from apple treescontaining the stem pitting agent[J].PlantD isease,1990,74:610-614.
- [9]M alinowskiI,K om orowska B,G olisT,etal.D etection ofapple stem pitting virusand pearvein yellows virus using reverse transcription-polym erase chain reaction[J].A cta H orticulturae,1998,472:87-95.