砂梨上苹果茎痘病毒的调查分析及RT-PCR与TC-RT-PCR检测研究Investigation and detection of Apple stem pitting virus in Pyrus pyrifolia by RT-PCR and TC-RT-PCR
侯雨萱,洪霓,邓晓云,胡红菊,王国平
摘要(Abstract)：
试验通过西方烟(Nicotianaoccidentalis‘37B’)和杂种(Pyroniaveitchii)、A20接种鉴定及PAS-ELISA检测,对保存于国家砂梨种质资源圃的38份砂梨样品进行了苹果茎痘病毒(Applestempittingvirus,ASPV)带毒率的调查分折。结果表明,生物学鉴定与血清学检测结果一致,ASPV带毒率均为26.3%。应用以dsRNA为模板RT-PCR检测该病毒,在杂种和A20上显症的6个样品均获得316bp目标片段。应用TC-RT-PCR检测显症的西方烟,也获得了预期的目标片段。
关键词(KeyWords)： 苹果茎痘病毒;生物学鉴定;PAS-ELISA;dsRNA;RT-PCR;TC-RT-PCR
基金项目(Foundation): 科技部863课题(项目编号:2001AA241142)。
作者(Author): 侯雨萱,洪霓,邓晓云,胡红菊,王国平
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