野生毛花猕猴桃雄株居群花粉形态观察Observation on the pollen morphology of male geotypes in wild Actinidia eriantha population
钟敏;谢敏;张文标;陶俊杰;黄春辉;朱博;徐小彪;
摘要(Abstract):
【目的】分析江西省麻姑山自然保护区内野生毛花猕猴桃花粉的形态特征,了解其遗传变异性、亲缘关系与演变规律。【方法】利用扫描电子显微镜对23个野生毛花猕猴桃雄株的花粉粒进行了形态观察。【结果】毛花猕猴桃雄株花粉粒外观为长球形,偶有近超长球形;极面观为三裂圆形,极轴长22.42~27.73μm,变异系数为4.75%;赤道轴长12.73~14.31μm,变异系数为3.12%。具3萌发沟,赤道面可见1~2条,等间距分布,萌发沟长20.52~24.68μm,变异系数为5.11%;萌发沟脊宽7.29~9.52μm,变异系数为6.78%;根据NPC分类系统分类,属于N3P4C5型花粉。花粉外壁纹饰有3种类型:脑纹状、波纹状和疣状纹饰,分别占花粉样品的65.22%、30.43%和4.35%。聚类分析结果显示,多数雄株亲缘关系较近。【结论】该居群内野生毛花猕猴桃雄株花粉的形态有较多的共性特征,同时在花粉的形态、外壁纹饰上,不同单株间存在差异,分别呈现毛花猕猴桃的遗传保守性和多样性。花粉外壁纹饰的演化趋势为:由波纹状向疣状和脑纹状演化。
关键词(KeyWords): 野生毛花猕猴桃;花粉形态;遗传多样性
基金项目(Foundation): 国家自然科学基金(31360472);; 国家科技基础性工作专项(2012FY110100-7);; 江西省重大科技专项(20143ACF60015);; 江西省现代农业产业技术体系建设专项(JXARS-05);; 研究生创新专项基金(NDYC2015-B002)
作者(Author): 钟敏;谢敏;张文标;陶俊杰;黄春辉;朱博;徐小彪;
Email:
DOI: 10.13925/j.cnki.gsxb.20160073
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