阎国华,周宇,张开春

• 1:北京市农林科学院林业果树研究所
• 2:北京市农林科学院林业果树研究所
• 3:北京市农林科学院林业果树研究所 北京100093
• 4:北京100093

摘要(Abstract)：

综述了近年来国内外核果类果树转基因的研究进展,涉及的内容有:用农杆菌转化核果类果树中难生根种类,获得易繁殖的转化植物;转移病毒外壳蛋白基因和杀菌毒性肽基因以提高抗病性;转移细胞分裂素基因和光敏素基因改良品种或砧木生长特性,培育紧密型的、短枝的、矮化半矮化的新品种;转移抗冻基因提高抗霜冻性;通过反义转基因技术调节果实的成熟,获得耐贮运的新性状,以及核果类果树基因转化技术的发展等方面。

关键词(KeyWords)： 核果类果树;转基因

Abstract:

Keywords:

基金项目(Foundation): 北京市科委科技新星计划项目资助

作者(Author): 阎国华,周宇,张开春

Email:

DOI:

参考文献(References)：

• 犤1犦RuginiE.Progressinstudiesoninvitrocultureofalmonds.Proc41stConfonPlanttissuecultureanditsagriculturalapplications犤C犦.Nottingham,1984.73.
• 犤2犦StrobelGA,NachmiasA.Agrobacteriumrhizogenespromotestheinitialgrowthofbarerootstockalmond犤J犦.JGenMicrobiol,1985,131:1245～1249.
• 犤3犦RuginiE,MariottiD.AgrobacteriumrhizogenesT-DNAgenesandrootinginwoodyspecies犤J犦.ActaHort,1992,300:301～308.
• 犤4犦DamianoC,ArchillettiT,CaboniE,etal.Agrobacterium-mediatedtransformationofalmond:invitrorootingthroughlocalizedinfectionofA.rhizogenes犤J犦.ActaHort,1995,392:161～169.
• 犤5犦DamianoC,CaboniE,MonticelliS,etal.1996invitrorootingoffruittreesbyAgrobacteriumrhizogenes:thecaseofalmondandapple.Proc2ndIntSympBiologyofrootformationanddevelopment犤A犦.In:AltmanA,WaiselAE(eds)Biologyofrootformationanddevelop-ment犤C犦.Plenum,NewYork,1997.
• 犤6犦CaboniE,DamianoC,FalascaG,etal.Rootingimprovementinwal-nutandalmondthroughinvitrolocalizedinfectionwithAgrobac-teriumrhizogenes.41stIntCongrEurTissuecultureSoc犤C犦.Verona,Italy,1994.
• 犤7犦MonticelliS,DamianoC,SciarroniR.InvitrolocalizedinfectionA-grobacteriumrhizogenesandrootinginfruittreesspecies犤M犦.MedFacLandbouwwUnivGent,1997.210～217.
• 犤8犦BassiG,CossioF,SansaviniS.AdventitiousrootformationinvitroshootsoffruittreesthoughengineeredAgrobacteriaandwildtypetoimproverootingability犤C犦.ProcAgroBioUniversitadelgliStudidiBologna,Italy,1993.21～28.
• 犤9犦HaapalaT,SantiniL,MariottiD.Agrobacterium-mediatedtransfor-mationintrees:Preliminarystudiesontransferofrolgenesintosomenortheuropeanwoodyspecies犤J犦.AdvHorticSci,1994,8:25～28.
• 犤10犦LaimerdaCamara,MachadoM,daCamaraMachadoA,etal.Expre-sionoftheplumpoxviruscoatproteingeneinNicotianaclevelandii犤J犦.ActaHort,1990,280:577～580.
• 犤11犦LaimerM,daCamaraMachadoA,MattanovichD,etal.Protectionagainstplumpoxpotyvirusbytransgeniccoatprotein犤C犦.VIIIntCongrPlanttissueandcellculture.Amsterdam,1990.178.
• 犤12犦LaimerdaCamaraMachado,daCamaraMachadoA,HenzerV,etal.RegenerationoftransgenicplantsofPrunusarmeniacacontainingthecoatproteingeneofplumpoxvirus犤J犦.PlantCellRep,1992,11:25～29.
• 犤13犦ScorzaR,RavelonandroM,CallahanA,etal.Transgenicplumsex-presstheplumpoxviruscoatproteingene犤J犦.PlantCellRep,1994,14:18～22.
• 犤14犦QuemadaH,L'HostisB,GonsalvesD,etal.Thenucleotidese-quenceofthe3'-terminalregionsofpapayaringspotvirusstrainsWandP犤J犦.JGenVirol,1990,71:203～210.
• 犤15犦ScorzaR,CordtsJM,ManteS,etal.Agrobacterium-mediatedtransformationofPlumwiththepapayaringspotviruscoatprotein犤J犦.HortScience,1991,26:786.
• 犤16犦ScorzaR,LevyL,DamsteegtV,etal.Transformationofplumwiththepapayaringspotviruscoatproteingeneandreactionoftransgenicplanttoplumpoxvirus犤J犦.JAmSocerHortSci,1995,120:943～952.
• 犤17犦SmigockiAC,HammerschlagFA.RegenerationofplantsfrompeachembryocellinfectedwithashootymutantstrainofAgrobacterium犤J犦.JAmSocHortSci,1991,116:1092～1097.
• 犤18犦Gutierrez-PesceP,TaylorK,MuleoR,etal.SomaticembryogenesisandshootregenerationfromtransgenicrootsofthecherryrootstockColt(P.avium×P.pseudocerasus)mediatedbypRi855T-DNAofAgrobacteriumrhizogenes犤J犦.PlantCellRep,1998,17:574～580.
• 犤19犦NegriP,MagnaniniE,CantoniL,etal.Genetransferinwoodyfruitspecies:initialtrailsingrowthhabitcontrol.ProcAgroBioFrut犤M犦.Cesena,Italy,1998.12～13.
• 犤20犦MuleoR,IaconaC.Architectureregulationbyphytochrome,pheno-typicevidanceincherryplanttransformedwithphytochromeAofrice.ProcAgroBioFrut犤M犦.Cesena,Italy,1998.14～15.
• 犤21犦CallahanAM,MorgensPH,WaltonE.Isolationandinvitrotrans-lationofRNAsfromdevelopingpeachfruit犤J犦.HortiScience,1989,24:356～358.
• 犤22犦CallahanAM,Morgens,PH,CohanRA,etal.ExpressionofPCH313duringfruitsofteningandtissuewounding犤J犦.HortiScience,1990,25:472.
• 犤23犦CallahanAM,MorgensPH,WrightP,etal.Acandidategenefortheextensionofpeachfruitshelflife犤J犦.JCellBiochem,1990,(Suppl):14E,R505/346.
• 犤24犦CallahanAM,ScorzaR,MorgensPH,etal.Breedingforcoldhardi-ness:searchforgenestoimprovefruitqualityincold-hardypeachgermplasm犤J犦.HortScience,1991,6:522～526.
• 犤25犦TrainottiL,SpolaoreS,FerrareseL,etal.CharacterisationofppRGI,anummberofmultigenefamilywhichencodesendo-bate1,4glu-canaseinpeach犤J犦.PlantMolBiol,1997,34:791～802.
• 犤26犦金勇峰,张耀洲,陈大明,等.桃ACC氧化酶基因的克隆和植物表达载体的构建犤J犦.园艺学报,1998,25(1):37～43.
• 犤27犦金勇峰,张耀洲.桃果实ACC合酶DNA的克隆犤J犦.园艺学报,2000,27(4):257～262.
• 犤28犦ZhangK-C,ZhangJ-K.CloningandsequencingofPGIPgenefromPrunusmahaleb.犤GenBank犦ACCESSIONAF263465,VER-SIONAF263465.1GI:8778049.
• 犤29犦DolgovSV.Genetictransformationofsourcherry(CerasusvulgarisMill.)leafdiscs.In:BajajYPSBiotechnologyinAgriculturalandForestry,Transgenictrees犤M犦.1999,44:29～38.
• 犤30犦DolgovSV,Firson.Regenerationandgenetictransformationofsourcherry(CerasusvulgarisMill.)leafdiscs犤J犦.ActaHort,1999,84.
• 犤31犦MirandaBrasileiroAC,LepleJC,MuzzinJ,etal.Analternativeap-proachforgenetransferintreesusingwildtypeAgrobacteriumstrains犤J犦.PlantMolBiol,1991,17:441～452.
• 犤32犦daCamaraMacnadoA,LaimerdaCamaraMachadoM.GenetictransformationinPrunusarmeniacaL.(apricot).In:BiotechnologyinAgriculturalandForestry,Plantprotoplastsandgeneticengineer-ingVI犤M犦.Springer,1995.240～254.
• 犤33犦DruartP,DelporteF,BrazdaM,etal.Genetictransformationofcherrytrees犤J犦.ActaHort,1998,468:71～76.
• 犤34犦daCamaraMacnadoA,DruartP,BrazdaM,etal.Routinetransfor-mationviasecondaryembryogenesisincherryrootstocks.IXIntCon-grPlanttissueandcellculture:plantbiotechnologyandinvitrobiol-ogyin21stcentury犤C犦.Jerusalem.Israel,1998.14～19.
• 犤35犦ScorzaR,MorgensPH,CordtsJM,etal.Agrobacterium-mediatedtransformationofpeachleafsegments,immatureembryos,andlongtermembryogeniccallus犤J犦.InVitroCellDevBiol,1990,26:829～834.
• 犤36犦ManteS,MorgensPH,ScorzaR,etal.Agrobacterium-mediatedtransformationofplum(PrunusdomesticaL.)hypocotylandregener-ationoftransgenicplants犤J犦.Bio/Technology,1991,9:853～857.
• 犤37犦ArchillettiT,LauriR,DamianoC.Agrobacterium-mediatedtrans-formationofalmondleafpieces犤J犦.PlantCellRep,1995,14:267～272.
• 犤38犦MiguelCM,OliveiraMM.TransgenicalmondplantsobtainedbyAgrobacterium-mediatedtransformationofleafexplants犤J犦.PlantCellRep,1999,18:387～393.
• 犤39犦RuginiE,MuganuM.Anovelstrategyfortheinductionandmainte-nanceofshootregenerationfromcallusderivedfromestablishedshootsofapple(MalusdomesticaBorkh)cv.GoldenDelicious犤J犦.PlantCellRep,1998,17:581～585.
• 犤40犦RuginiE,MuganuM,Gutierrez-PesceP,etal.Strategiestoinducemorphogenesisandevaluationofsomefruitspeciestransformedwithrolandosmotingenes.ProcAgroBioFrut犤M犦.Cesena,Italy,1998.8～9.
• 犤41犦RuginiE,CaricatoG,MuganuM,etal.Geneticstabilityandagro-nomicevaluationof6-year-oldtransgenickiwiplantsforrolABCandrolBgenes犤J犦.ActaHort,1997,447:609～610.
• 犤42犦RavelonandroM,ScorzaR,BachelierJC,etal.Resistanceoftrans-genicPrunusdomesticatoplumpoxvirusinfection犤J犦.PlantDis,1997,81:1231～1235.