刘韬;车建美;黄素芳;廖汝玉;刘波;

• 1:福建省农业科学院

摘要(Abstract)：

采用RAPD技术对17份杨桃品种资源进行遗传多样性分析。结果表明,13条随机引物共扩增出80条RAPD带,其中77条为多态性带,平均多态位点5.92条,多态率达97.19%,表明杨桃遗传多样性的复杂性。利用UPGMA构建了杨桃的亲缘关系树状图,遗传距离为0~2.160 93,当λ=2.08时,17份杨桃品种可以划分为3个类群,第Ⅰ类群中的杨桃品种基本都属于甜杨桃品种,第Ⅱ类群中的杨桃品种既包含了甜杨桃也包含了酸杨桃品种,第Ⅲ类群中则只包含了云霄3号一个品种。

关键词(KeyWords)： 杨桃(Averrhoa carambola L.);RAPD;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 福建省科技厅重点项目(No.2007N0032);福建省科技厅重点项目(No.2008I0007);; 福建省财政专项-福建省农业科学院科技创新团队建设基金(No.STIF-Y03);; 公益性行业(农业)科研专项(No.200903034)

作者(Author): 刘韬;车建美;黄素芳;廖汝玉;刘波;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.03.019

参考文献(References)：

• [1]LIU Sheng-hui,WEI Chang-bin,LI Wei-cai,ZANG Xiao-ping.Analysis of the aromatic constituents in 3 starfruit cultivar[J].Journalof Fruit Science,2008,25(1):119-121.刘胜辉,魏长宾,李伟才,臧小平.3个杨桃品种的果实香气成分分析[J].果树学报,2008,25(1):119-121.
• [2]ZHONG Yun,JIANG Bo,JIANG Nong-hui,LIU Yan,LIU Chuan-he,ZENG Fang,YI Gan-jun.Quality analysis and determination ofoxalic acid content of diferent varieties of carambola[J].GuangdongAgricultural Sciences,2009(12):67-69.钟云,姜波,蒋侬辉,刘岩,刘传和,曾芳,易干军.不同杨桃品种品质分析及草酸含量的测定研究[J].广东农业科学,2009(12):67-69.
• [3]LIAO Ru-yu,LIU Tao,CAI Yuan-cheng,HUANG Su-fang,CHENTian-you,YANG Ling,HUANG Jin-xun.The situation,problemand development contermeasures of the sweet carambola trade in Fu-jian provinc[J].Chinese Agricultural Science Bulletin,2009,25(20):213-215.廖汝玉,刘韬,蔡元呈,黄素芳,陈天佑,杨凌,黄金勋.福建省甜杨桃产业现状、存在问题及发展对策[J].中国农学通报,2009,25(20):213-215.
• [4]LI Huan-ling,LAI Zhong-xiong,CHEN Yi-ting,LIN Xiu-lian,XU Wei,LIN Zhi-liang.Rapd analysisi of genetic diversity of an-cient Litchi trees[J].Chinese Journal of Tropical Crops,2009,30(4):450-455.李焕苓,赖钟雄,陈义挺,林秀莲,徐炜,林治良.66份荔枝古树遗传多样性的RAPD分析[J].热带作物学报,2009,30(4):450-455.
• [5]CHEN Yi-ting,LAI Zhong-xiong,CHEN Jing-ying,CAI Ying-qing,GUO Yu-qiong,WU Jin-shou.RAPD analysis of 65 acces-sions of loquat germplasm(Eriobotrya spp.)[J].Chinese Journal ofTropical Crops,2007,28(1):65-71.陈义挺,赖钟雄,陈菁瑛,蔡英卿,郭玉琼,吴金寿.65份枇杷种质资源的RAPD分析[J].热带作物学报,2007,28(1):65-71.
• [6]YANG Xiang-hui,LI Ping,LIU Cheng-ming,LIN Shun-quan.Ge-netic diversity in Eriobotrya genus and its closely related plantspecies using RAPD markers[J].Journal of Fruit Science,2009,26(1):55-59.杨向晖,李平,刘成明,林顺权.枇杷属植物及其近缘属植物亲缘关系的RAPD分析[J].果树学报,2009,26(1):55-59.
• [7]CAI Ying-qing,LAI Zhong-xiong,CHEN Yi-ting,GUO Yu-qiong,PAN Dong-ming.RAPD analysis of emblic(Phyllanthusemblica L.)genetic resources in Fujian province[J].Chinese Journalof Tropical Crops,2007,28(2):74-79.蔡英卿,赖钟雄,陈义挺,郭玉琼,潘东明.福建余甘子遗传资源的RAPD分析[J].热带作物学报,2007,28(2):74-79.
• [8]ARVIND K A,NAGARAJA G M,NAIK G V,SRIRAMANA K,THANGAVELU K,JAVAREGOWDA N.Genetic diversity and re-lationships in mulberry(genus Morus)as revealed by RAPD and IS-SR marker assays[J].BMC Genetics,2004,5:1-9.
• [9]IOANA M,LEHEL L,MARIN A,VIOREL M,RADU S,RODICAP,MIRELA C.Genotypic variability of the main apple cultivarsgrown in Transylvania,Romania,evaluated by means of RAPDanalysis[J].Not Bot Hort Agrobot Cluj,2009,37(1):261-264.
• [10]ZHANG Chang-yuan,SUN Ni,HU Kai-lin.RAPD analysis in ge-netic relationship among varieties of balsam pear[J].Molecular PlantBreeding,2005,3(4):515-519.张长远,孙妮,胡开林.苦瓜品种亲缘关系的RAPD分析[J].分子植物育种,2005,3(4):515-519.
• [11]OLIVERIA C M,MOTA M,MONTE C,GOULO L,SILVA D M.Molecular typing of Pyrus based on RAPD markers[J].ScientiaHorticulturae,1999,79:163-174.
• [12]LI Yuan-yuan,GUO Xiu-wu,DAI Han-ping,LIU Hai-tao.Analysison 31 wild raspberries with RAPD markers[J].Journal of Fruit Sci-ence,2009,26(2):240-244.李媛媛,郭修武,代汉萍,刘海涛.31份野生树莓资源的RAPD分析[J].果树学报,2009,26(2):240-244.
• [13]LI Li,LIU Yu-xiang,PENG Jian-ying.Identification and analysison several new peach cultivars by RAPD markers[J].Journal of Agri-cultural University of Hebei,2010,33(1):30-33.李莉,刘玉祥,彭建营.几个桃优质新品种的RAPD鉴定与分析[J].河北农业大学学报,2010,33(1):30-33.
• [14]LIN Xiao-hong,CHEN Qing-xi,LIN Feng-ying,WU Pei-can,ZHENG Hao.The investigation of the adaptability and introductionof Taiwan’s soft-branch carambola(Psidium guajava L.)[J].ChineseAgricultural Science Bulletin,2005,21(6):270-272.林晓红,陈清西,林丰英,吴培灿,郑浩.台湾软枝杨桃的引种及其适应性研究[J].中国农学通报,2005,21(6):270-272.
• [15]CHENG Zhi-ming,CHEN Qing-xi,LAN Zhi-fu,WANG Yu-ling.The investigation of the adaptability and cultivation techniques ofTaiwan’s carambola in Zhangzhou[J].Fujian Fruit,2004(1):12-14.程志明,陈清西,蓝志福,王玉玲.台湾杨桃在漳州的适应性与栽培技术要点[J].福建果树,2004(1):12-14.
• [16]FRANKE O H,BROWN A H D.Current plant genetic resources-acritical appraisal.Genetics:New Frontiers Vol.Ⅳ[M].New Delhi:Ox-ford and IBH Publishing,1984:1-13.
• [17]JIANG Hui-fang,REN Xiao-ping,HUANG Jia-quan,LIAO Bo-shou,LEI Yong.Establishment of peanut mini core collection inChina and exploration of new resource with high oleat[J].ChineseJournal of Oil Crop Science,2008,30(3):294-299.姜慧芳,任小平,黄家权,廖伯寿,雷永.中国花生小核心种质的建立及高油酸基因源的发掘[J].中国油料作物学报,2008,30(3):294-299.
• [18]ZONG Xu-xiao,GUAN Jian-ping,GU Jing,WANG Hai-fei,MAYu.Differentiation on population structure and genetic diversity ofpea core collections separately constituted from Chinese landracesand international genetic resources[J].Journal of Plant Genetic Re-sources,2009,10(3):347-353.宗绪晓,关建平,顾竞,王海飞,马钰.中国和国际豌豆核心种质群体结构与遗传多样性差异分析[J].植物遗传资源学报,2009,10(3):347-353.
• [19]PANG Bin-shuang,LI Li-hui,JIA Ji-zeng.Establishment of candi-date core collections in Chinese common wheat germplasm[J].Jour-nal of Plant Genetic Resource,2003,4(1):1-8.庞斌双,李立会,贾继增.中国普通小麦初选核心种质的产生[J].植物遗传资源学报,2003,4(1):1-8.
• [20]LI Jin-hui,LI Ming-lei.Charicterics and ornamental plants pottedof Tainong 7 and Malaysian B17[J].Fujian Fruit,2008(2):33-35.李晋辉,李明磊.台农1号和马来西亚B17甜阳桃特征特性及盆栽技术[J].福建果树,2008(2):33-35.
• [21]WU Ren-shan.Introduction of some sweet carambola cultivar[J].Guangxi Horticulture,2002(3):27.吴仁山.介绍几个甜杨桃优良品种[J].广西园艺,2002(3):27.
• [22]WILLIAMS J G,KUBELIK A R,LIVAK K J,RAFALSKI J A,TINGEYL S V.DNA polymorphisms amplified by arbitrary primersare useful as genetic markers[J].Nucleic Acid Research,1990,18(22):6531-6535.
• [23]WELSH J,MCCLELLAND M.Fingerprinting genomes using PCRwith arbitary primers[J].Nuclei Acids Research,1990,18(24):7213-7218.
• [24]QIU Fang,FU Jian-min,JIN De-min,WANG Bin.The moleculardetection of genetic diversity[J].Chinese Biodiversity,1998,6(2):143-150.邱芳,伏健民,金德敏,王斌.遗传多样性的分子检测[J].生物多样性,1998,6(2):143-150.
• [25]SHANG Zhi-qiang.Progress of the application research related ofmolecular markers in tobacco to germplasm resource[J].Biotechnolo-gy Bulletin,2010(3):58-61.尚志强.分子标记技术在烟草种质资源研究中的应用进展[J].生物技术通报,2010(3):58-61.
• [26]HAO Bao-jun,WANG Bao-tong,LI Qiang,LI Gao-bao,WANGFang,ZHANG Bo,KANG Zhen-sheng.RAPD analysis and SCARmarker establishment of Su 11 group of Puccinia striiformis f.sp.tritici in China[J].Acta Phytopathologica Sinica,2010(1):1-6.郝保军,王保通,李强,李高宝,王芳,张勃,康振生.小麦条锈菌水源11类群的RAPD分析及SCAR标记的建立[J].植物病理学报,2010(1):1-6.
• [27]LIANG Hui-min.Analysis on different populations of cynodondactylon by RAPD[J].Acta Prataculturae Sinica,2010,19(1):258-262.梁慧敏.不同居群狗牙根RAPD分析[J].草业学报,2010,19(1):258-262.