王宏;蔺经;李晓刚;王中华;常有宏;

• 1:江苏省农业科学院园艺研究所·江苏省高效园艺作物遗传改良重点实验室

摘要(Abstract)：

【目的】分离和克隆杜梨ABA受体蛋白基因Pb PYL4,了解其在杜梨响应盐胁迫的表达情况。【方法】以杜梨(Pyrus betulaefolia Bunge)无性系组培苗为试验材料,使用RT-PCR的方法从杜梨中克隆到ABA受体基因,命名为PbPYL4,并研究该基因盐胁迫处理下的表达模式,同时检测ABA合成基因Pb NCED2在盐胁迫下的表达模式。【结果】PbPYL4的c DNA长度为682 bp,其中开放阅读框(ORF)621 bp,编码207个氨基酸的蛋白。系统发生分析结果表明,PbPYL4与植物中已经发现的拟南芥ABA受体蛋白PYL4/RCAR10亲缘关系较近。荧光定量RT-PCR表达分析结果表明,根,盐胁迫12 h内,Pb PYL4基因被诱导表达,12 h达到第1个小高峰,随后24 h表达下降,48 h表达又上升,72 h达到第2个峰值;茎,盐胁迫48 h后,Pb PYL4基因被诱导表达;叶片,处理12 h后,Pb PYL4基因被诱导表达,72 h表达略微下降。在同样组织中,Pb NCED2表现出与Pb PYL4相似的表达时序。【结论】Pb PYL4基因可能在杜梨响应盐胁迫的过程中起了重要的作用。

关键词(KeyWords)： 杜梨;ABA受体PbPYL4;基因克隆;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31300469);; 江苏省农业科技自主创新[CX(13)5105,CX(14)5016];; 江苏省自然科学基金(BK2014021063)

作者(Author): 王宏;蔺经;李晓刚;王中华;常有宏;

Email:

DOI: 10.13925/j.cnki.gsxb.20140138

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