宋雅林;林苗苗;钟云鹏;陈锦永;齐秀娟;孙雷明;方金豹;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】分析不同猕猴桃品种(系)对细菌性溃疡病的抗性以及不同评价指标间的相关性,对鉴定猕猴桃种质资源的抗溃疡病能力和进一步开展抗病相关育种具有非常重要的意义。【方法】利用收集的溃疡病病原菌[Pseudomonas syringae pv. Actinidiae(Psa)],经牛肉膏蛋白胨培养基培养,通过猕猴桃离体枝条接种方法和观察接种后的浸染情况,分析了不同猕猴桃品种(系)的抗病性以及病情指数和病斑长度之间的相关性;并用流式细胞仪检测供试材料的倍性,分析抗病性与倍性关系。【结果】不同品种(系)对溃疡病的抗病性存在显著差异,在29份猕猴桃种质资源中‘,华特’等3份毛花猕猴桃材料表现为高抗;‘徐香‘’金魁’等15份种质资源表现为抗病;‘金桃‘’金艳’等4份种质资源表现为耐病;‘Hort16-A’等5份材料表现为感病;中华猕猴桃‘红阳’和‘2-72’表现为高感。不同品种(系)的病情指数与病斑长度呈极显著正相关,相关系数r为0.957。此外,发现在中华猕猴桃中,高感品种‘红阳’‘2-72’和感病品种‘1-74’‘Hort16-A’是二倍体,耐病品种‘金桃’和‘金艳’是四倍体。【结论】抗性评价结果与生产实际发病情况基本相符,说明猕猴桃离体枝条接种方法能够有效的对猕猴桃品种抗病性进行评价,病斑长度可作为重要的评价指标;同一类型品种(系)染色体组倍性越大,抗病性越强。

关键词(KeyWords)： 猕猴桃;溃疡病;品种(系);抗性;相关性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金(31701778);; 国家重点研发计划(2019YFD1000200);; 河南省科技攻关计划(182102110411);; 河南省现代农业产业技术体系(S2014-11);; 国家园艺种质郑州猕猴桃分库(NHGRC2020-NH00-4);; 河南省财政林木种质资源项目

作者(Author): 宋雅林;林苗苗;钟云鹏;陈锦永;齐秀娟;孙雷明;方金豹;

Email:

DOI: 10.13925/j.cnki.gsxb.20190060

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