牛姣,周存田,王西平

• 1:西北农林科技大学园艺学院·农业部西北地区园艺作物生物学与种质创制重点开放实验室
• 2:旱区作物逆境生物学国家重点实验室

摘要(Abstract)：

【目的】为了验证COI1基因在葡萄抗病防御机制中的作用,【方法】在前期通过抑制消减杂交技术(SSH)获得中国野生葡萄毛葡萄株系‘商-24’COI1基因EST序列的基础上,采用RT-PCR从毛葡萄‘商-24’中分离到COI1基因cDNA序列,并通过实时定量PCR和半定量PCR分析了COI1基因的表达特异性,以期验证其在葡萄抗病防御机制中的作用。【结果】序列分析表明,VqCOI1基因长2 455 bp,编码598个氨基酸残基,相对分子量为67.87 ku,等电点为5.85。同源性分析表明,它与已克隆的大豆(Glycine max)COI1基因(GenBank登录号:AAZ66745.1)相似性最高为79%。实时定量PCR技术用来检测VqCOI1基因在受到病原菌侵染和激素处理时在抗病品种‘商-24’中的表达情况,结果表明,在白粉菌诱导初期,抗病的毛葡萄株系‘商-24’VqCOI1基因表达量升高,这可能是因为它参与了病原菌诱导的JA信号调节途径。水杨酸(SA)、茉莉酸甲酯(MeJA)、乙烯(Eth)处理表明VqCOI1基因表达受激素分子调节,这些信号分子可能交叉调节这一基因的表达。在嫩叶、嫩茎、花、果皮、卷须等不同葡萄组织中的表达表明Vq-COI1基因的表达具有空间差异性。【结论】这些结果暗示着VqCOI1的表达响应病原菌侵染并受生物胁迫相关激素的调控,很有可能在葡萄防御病原菌侵染的机制中发挥重要作用。

关键词(KeyWords)： 中国野生葡萄;VqCOI1基因;克隆;表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(30871701,31071782)

作者(Author): 牛姣,周存田,王西平

DOI: 10.13925/j.cnki.gsxb.2012.06.012

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