林苗苗;赵长竹;顾红;张慧琴;陈锦永;谢鸣;方金豹;

• 1:中国农业科学院郑州果树研究所·中国农业科学院果树生长发育与品质控制重点开放实验室
• 2:浙江省农业科学院园艺研究所

摘要(Abstract)：

为研究MADS-box基因在甜樱桃花发育中的作用,以甜樱桃品种拉宾斯为试材,根据多种植物MADS-box基因保守序列设计引物,采用RT-PCR和RACE方法克隆基因cDNA全长,并用半定量PCR法研究基因在不同组织中的表达。克隆得到1个基因全长962 bp,命名为PaMADS2(GenBank登录号:HQ229606),包含1个633 bp的开放阅读框,编码210个氨基酸。序列比对和进化分析表明,PaMADS2与B类的PI基因家族亲缘关系最近。组织特异性分析表明PaMADS2在雄蕊和花瓣中表达。推断所克隆的PaMADS2基因为MADS-box B类家族成员。

关键词(KeyWords)： 甜樱桃;花器官;雄蕊;花瓣;MADS-box

Abstract:

Keywords:

基金项目(Foundation): 十一五科技支撑资助项目(2006BAD07B06-10);; 浙江省科技计划面上项目(2009C32031);; 中国农业科学院果树生长发育与品质控制重点开放实验室资助项目(PP333KF20090101)

作者(Author): 林苗苗;赵长竹;顾红;张慧琴;陈锦永;谢鸣;方金豹;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.06.034

参考文献(References)：

• [1]NORMAN C,RUNSWICK M,POLLOCK R,TREISMAN R.Isola-tion and properties of cDNA clones encoding SRF,a transcriptionfactor that binds to the C-fos serum response element[J].Cell,1988,55(6):989-1003.
• [2]PASSMORE S,MAINE G T,ELBLE R,CHRIST C,TYE B K.Saccharomyces cerevisiae protein involved in plasmid maintenanceis necessary for mating of MAT alpha cells[J].J Mol Biol,1988,204(3):593-606.
• [3]SOMMER H,BELTRAN J P,HUIJSER P,PAPE H,LONNIG WE,SAEDLER H,SCHWARZ-SOMMER Z.Deficiens,a homeoticgene involved in the control of flower morphogenesis in Antirrhinummajus:the protein shows homology to transcription factors[J].TheEMBO Journal,1990,9(3):605-613.
• [4]SCHWARZ-SOMMER Z,HUE I,HUIJSER P,FLOR P J,HANSENR,TETENS F,LONNING W E,SAEDLER H,SOMMER H.Char-acterization of the Antirrhinum floral homeotic MADS-box gene de-ficiens:Evidence for DNA binding and autore-gulation of its persis-tent expression throughout flower deve-lopment[J].The EMBO Jour-nal,1992,11(1):251-263.
• [5]MUNSTER T,PAHNKE J,DI R A,KIM J T,MARTIN W,SAEDLER H,THEISSEN G.Floral homeotic genes were recruitedfrom homologous MADS-box genes preexisting in the common an-cestor of ferns and seed plants[J].PNAS,1997,94(6):2415-2420.
• [6]BECKER A,THEISSEN G.The major Glades of MADS-box genesand their role in the development and evolution of flowering plants[J].Molecular Phylogenetics Evolution,2003,29(3):464-489.
• [7]COEN E S,MEYEROWITZ E M.The war of the whorls:genetic in-teractions controlling flowerdevelopment[J].Nature,1991,353(6339):31-37.
• [8]YAO J,DONG Y,MORRIS B A M.Parthenocarpic apple fruit pro-duction conferred by transposon insertion mutations in a MADS-boxtranscription factor[J].PNAS,2001,98:1306-1311.
• [9]XU Yong,ZHANG Lin,XIE Hua,ZHANG Yan-qiu,OLIVEIRA MM,MA Rong-cai.Expression analysis and genetic mapping of threeSEPALLATA-Like genes from peach(Prunus persica(L.)Batsch)[J].Tree Genetics&Genomes,2008,4:693-703.
• [10]BEPPU K,SUEHARA T,KATAOKA I.Embryo sac developmentand fruit set of Satonishiki sweet cherry as affected by temperature,GA3 and paclobutrazol[J].Journal of Japanese Society for Horticul-tural Science,2001,70(2):157-162.
• [11]WANG Shi-ping,YUAN Cai-juan,DAI Yun-ting,SHU Yuan,YANG Tian-yi,ZHANG Cai-xi.Development of flower organs insweet cherry in shanghai area[J].Acta Horticulturae Sinica,2004,31(3):357-359.王世平,袁彩娟,戴韵婷,舒缓,杨天仪,张才喜.栽培的欧洲甜樱桃花器官发育的观察[J].园艺学报,2004,31(3):357-359.
• [12]BIAN Wei-dong.Characterization of the flowering and fruit set ofsweet cherry in greenhouse[D].Yangling:Northwest A&F University,2008.边卫东.温室甜樱桃开花结实特性研究[D].杨凌:西北农林科技大学,2008.
• [13]JIANG Jian-fu.Flower bud differeniation in sweet cherry and the In-fluence of temperature[D].Beijing:The Chinese Academy of Agri-cultural Sciences,Master Dissertation,2008.姜建福.甜樱桃花芽分化及温度对其影响的研究[D].北京:中国农业科学院,2008.
• [14]SAITOU N,NEI M.The neighbor-jorning method:a new method forreconstructing phylogenetic trees[J].Molecular Biology and Evolu-tion,1987,4(10):406-425.
• [15]FAN Jin-hui,CHEN Jing,LI Wen-qing,LI Qing,ZOU Qi.Isola-tion and functional analysis of a MADS Box gene in populus tomen-tosa[J].Scientia Silvae Sinicae,2007,43(4):36-42.樊金会,陈静,李文卿,李青,邹琦.一个毛白杨MADS盒基因的克隆与功能分析[J].林业科学,2007,43(4):36-42.
• [16]GOTO K,MEYEROWITZ E M.Function and regulation of the Ara-bidopsis floral homeotic gene PISTILLATA[J].Genes Dev,1994,8:1548-1560.
• [17]TANAKA N,WADA M,KOMORI S,BESSHO H,SUZUKI A.Functional analysis of MdPI,the PISTILLATA gene homologue ofapple,in Arabidopisis[J].J Japan Soc Hort Sci,2007,76(2):125-132.
• [18]TIAN Hui-yu,CHEN Kao-shan,MENG Zheng.Isolation and Char-acterization of MADS-box Genes from Taihangia rupestri[D].Jinan:Shandong University,2003.田会玉、陈靠山、孟征.太行花MADS-box基因的克隆及其表达分析[D].济南:山东大学,2003.
• [19]LU Wen-liang.Development of sexual oragans in taihangia ru-pestris-different temperature requirments for both sexual organ de-velopment in a bisexual flower[J].Acta Botanica Sinina,1996,38(3):174-179.陆文梁.太行花性器官发育的研究—两性花中雌雄性器官发育对温度的不同要求[J].植物学报,1996,38(3):174-179.