林苗苗;赵长竹;顾红;张慧琴;陈锦永;谢鸣;方金豹;

• 1:中国农业科学院郑州果树研究所·中国农业科学院果树生长发育与品质控制重点开放实验室
• 2:浙江省农业科学院园艺研究所

摘要(Abstract)：

为研究MADS-box基因在甜樱桃花发育中的作用,以甜樱桃品种拉宾斯为试材,根据多种植物MADS-box基因保守序列设计引物,采用RT-PCR和RACE方法克隆基因cDNA全长,并用半定量PCR法研究基因在不同组织中的表达。克隆得到1个基因全长962 bp,命名为PaMADS2(GenBank登录号:HQ229606),包含1个633 bp的开放阅读框,编码210个氨基酸。序列比对和进化分析表明,PaMADS2与B类的PI基因家族亲缘关系最近。组织特异性分析表明PaMADS2在雄蕊和花瓣中表达。推断所克隆的PaMADS2基因为MADS-box B类家族成员。

关键词(KeyWords)： 甜樱桃;花器官;雄蕊;花瓣;MADS-box

Abstract:

Keywords:

基金项目(Foundation): 十一五科技支撑资助项目(2006BAD07B06-10);; 浙江省科技计划面上项目(2009C32031);; 中国农业科学院果树生长发育与品质控制重点开放实验室资助项目(PP333KF20090101)

作者(Author): 林苗苗;赵长竹;顾红;张慧琴;陈锦永;谢鸣;方金豹;

Email:

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