张美鑫;翟立峰;周玉霞;陈晓忍;贾娜娜;洪霓;王国平;

• 1:华中农业大学植物科学技术学院·湖北省作物病害监测与安全控制重点实验室
• 2:华中农业大学·农业微生物学国家重点实验室

摘要(Abstract)：

【目的】研究明确我国梨树腐烂病菌致病力的分化状况。【方法】梨离体枝条经打孔法造成伤口后接种梨树腐烂病菌,通过接种在5个梨系统(白梨、沙梨、秋子梨、西洋梨和新疆梨)不同品种上观测和比较不同来源菌株的致病力差异,并根据各菌株的综合致病力强弱划分为不同类型,分析致病力分化与菌株来源间的相关性。【结果】研究发现来源于我国14省市5个梨系统的91份梨树腐烂病菌株在不同梨品种上的致病力均存在显著性差异,但同一菌株在5个梨品种上的症状反应程度有异。根据各供试菌株在不同梨品种上的综合致病力强弱可将其划分为3个类型,其中强致病力菌株16份,占17.6%;中致病力菌株70份,占76.9%;弱致病力菌株5份,占5.5%。来源于不同地区和梨系统的菌株之间其致病类型的分布比例存在明显的差异,这种差异可能与其梨系统的抗病性相关。【结论】来源于我国不同地区和梨系统的梨树腐烂病菌存在明显的致病力分化,其综合致病力可划分为强、中、弱3个致病类型,以中等致病力菌株为优势群体。

关键词(KeyWords)： 梨树腐烂病菌;菌株;致病力分化

Abstract:

Keywords:

基金项目(Foundation): 国家梨产业技术体系(CARS-29-10);; 国家农业行业公益性项目(201203034-02;200903004-05)

作者(Author): 张美鑫;翟立峰;周玉霞;陈晓忍;贾娜娜;洪霓;王国平;

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DOI:

参考文献(References)：

• [1]SUZAKI K.Population structure of Valsa ceratosperma,causal fun-gus of Valsa canker,in apple and pear orchards[J].J Gen Plant Pathol,2008,74:128-132.
• [2]YANG Ying-chun,MIAO Chun-hui.The occurrence regularity andprevention methods of pear Valsa canker[J].Hebei Fruits,2010(5):41.杨迎春,苗春会.梨树腐烂病发生规律与防治方法[J].河北果树,2010(5):41.
• [3]LU Yan-jun.The reasearch on pathogens of pear Valsa canker[J].Acta Phytopathologica Sinica,1992,22(3):197-203.陆燕君.梨树腐烂病病原菌的研究[J].植物病理学报,1992,22(3):197-203.
• [4]ADAMS G C,WINGFIELD M J,COMMON R,ROUX J.Phylogenet-ic relationships and morphology of Cytospora species and relatedteleomorphs(Ascomycota,Diaporthales,Valsaceae)from Eucalyptus[J].Studies in Mycology,2005,52:122-124.
• [5]WEI Jie-ling,HUANG Li-li,GAO Zuo-peng,KE Xi-wang,KANG Zhen-sheng.Laboratory evaluation methods of apple Valsacanker disease caused by Valsa ceratosperma sensu Kobayashi[J].Acta Phytopathologica Sinica,2010,40(1):14-20.韦洁玲,黄丽丽,郜佐鹏,柯希望,康振生.苹果树腐烂病室内快速评价方法的研究[J].植物病理学报,2010,40(1):14-20.
• [6]BESSHO H,TSUCHIYA S,SOEJIMA J.Screening methods of appletrees for resistance to Valsa canker[J].Euphytica,1994,77:15-18.
• [7]ZHANG Mei-xin,ZHAI Li-feng,ZHOU Yu-xia,CHEN Xiao-ren,JIA Na-na,HONG Ni,WANG Guo-ping.Laboratory determiningmethods of the pathogenicity of Valsa canker in pear[J].Journal ofFruit Science,2013,30(2):317-322.张美鑫,翟立峰,周玉霞,陈晓忍,贾娜娜,洪霓,王国平.梨腐烂病致病力的室内快速测定方法研究[J].果树学报,2013,30(2):317-322.
• [8]ZHOU Yu-xia,CHENG Li-jing,ZHANG Mei-xin,ZHAI Li-feng,HONG Ni,WANG Guo-ping,WANG Li-ping.The sequence anal-ysis and preliminary identification for the pathogen of the pear Valsacanker in China[J].Journal of Fruit Science,2013,30(1):140-146.周玉霞,程栎菁,张美鑫,翟立峰,洪霓,王国平,王利平.我国梨腐烂病病原菌的初步鉴定及序列分析[J].果树学报,2013,30(1):140-146.
• [9]WANG X L,WEI J L,HUANG L L,KANG Z S.Re-evaluation ofpathogens causing Valsa canker on apple in China[J].Mycologia,2011,103(2):317-324.
• [10]ABE K,KOTODA N,KATO H,SOEJIMA J.Genetic studies on re-sistance to Valsa canker in apple:genetic variance and breeding val-ues estimated from intra-and inter-specific hybrid progeny popula-tions[J].Tree Genetics&Genomes,2011,7:363-372.
• [11]WEI Jie-ling.Study on the pathogenicity of different isolates of appletree Valsa canker pathogen[D].Shaanxi:Northwest A&F University,2009.韦洁玲.苹果树腐烂病菌不同分离株致病性差异研究[D].陕西:西北农林科技大学,2009.
• [12]DU Lei,ZHANG Le,GAO Zhi-mou,WANG Ge,ZHANG Jia-xia.Studies on biological characteristics and pathogenicity differentiationin Altemaria alternata in Anhui[J].Acta Tabacaria Sinica,2009,15(1):39-43.杜雷,张乐,高智谋,王革,张家侠.安徽烟草赤星病菌生物学特性及致病力分化研究[J].中国烟草学报,2009,15(1):39-43.
• [13]LI Xi-ling,GAO Zhi-mou,LI Yan-mei,QIU Yan,LIU Xiao-yan,PENG Mei.Studies on differentiation of pathogenicity of Botrytiscinerea isolates from four hosts to strawberry[J].Acta HorticulturaeSinica,2009,36(9):1365-1369.李喜玲,高智谋,李艳梅,邱艳,刘小燕,彭梅.四种寄主来源的灰葡萄孢对草莓的致病力分化研究[J].园艺学报,2009,36(9):1365-1369.
• [14]PENG Bin.Differentiation of pathogenicity on branch and genetic di-versity of Botryosphaeria dothidea in Henan[D].Beijing:Chinese A-cademy of Agricultural Sciences Dissertation,2011.彭斌.河南省苹果轮纹病菌枝干致病力分化与遗传多样性[D].北京:中国农业科学院,2011.
• [15]WANG Ling,HUANG Wen-wen,HUANG Shi-wen,LIU Lian-meng,LIU En-yong.Pathogenicity differentiation of rice sheathblight pathogen Rhizoctonia solani AG-1 I A isolates from Anhuiand Hubei provinces in China[J].Chinese Journal of Rice Science,2010,24(6):623-629.王玲,黄雯雯,黄世文,刘连盟,刘恩勇.皖鄂地区水稻纹枯病菌致病力分化研究[J].中国水稻科学,2010,24(6):623-629.
• [16]NEERAJA C N,SHENOY V V,REDDY C S,SARMA N P.Isozymepolymorphism and virulence of Indian isolates of the rice sheathblight fungus[J].Mycopathologia,2002,156:101-108.