刘冰浩;邓崇岭;陈传武;邓光宙;丁萍;牛英;唐艳;付慧敏;

• 1:广西柑橘生物学重点实验室·广西特色作物研究院

摘要(Abstract)：

【目的】了解和研究广西地方柑橘资源的亲缘关系和遗传多样性。【方法】以10份外省野生和地方柑橘种质为参照,采用ISSR分子标记技术对10份广西地方柑橘种质和8份姑婆山野生柑橘资源的遗传多样性及其亲缘关系进行分析和评价。【结果】从100条ISSR引物中筛选出12条引物对供试材料进行多态性分析。获得总条带67条,其中多态性条带62条,多态位点百分率为92.54%。聚类结果显示28份资源可分为7个组,其中广西资源分布于6个组,姑婆山野生柑橘被聚为同一组,进而可划分为2个亚组。【结论】ISSR标记能较好地区分广西地方柑橘资源,且广西柑橘地方资源遗传多样性丰富。

关键词(KeyWords)： 柑橘资源;广西;地方;ISSR;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31160388);; 广西自然科学基金重点项目(2013GXNSFDA019014);广西自然科学基金项目(2013GXNSFBA019112);; 广西科学研究与技术开发计划项目(桂科合1347004-11);; 国家现代农业(柑橘)产业技术体系建设专项(CARS-27-04B);; 桂林市科技开发项目(20120118-11);; 广西柑橘生物学重点实验室项目(桂柑科201301X004;桂柑科201202k001)

作者(Author): 刘冰浩;邓崇岭;陈传武;邓光宙;丁萍;牛英;唐艳;付慧敏;

Email:

DOI: 10.13925/j.cnki.gsxb.20140407

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