刘冰浩;邓崇岭;陈传武;邓光宙;丁萍;牛英;唐艳;付慧敏;

• 1:广西柑橘生物学重点实验室·广西特色作物研究院

摘要(Abstract)：

【目的】了解和研究广西地方柑橘资源的亲缘关系和遗传多样性。【方法】以10份外省野生和地方柑橘种质为参照,采用ISSR分子标记技术对10份广西地方柑橘种质和8份姑婆山野生柑橘资源的遗传多样性及其亲缘关系进行分析和评价。【结果】从100条ISSR引物中筛选出12条引物对供试材料进行多态性分析。获得总条带67条,其中多态性条带62条,多态位点百分率为92.54%。聚类结果显示28份资源可分为7个组,其中广西资源分布于6个组,姑婆山野生柑橘被聚为同一组,进而可划分为2个亚组。【结论】ISSR标记能较好地区分广西地方柑橘资源,且广西柑橘地方资源遗传多样性丰富。

关键词(KeyWords)： 柑橘资源;广西;地方;ISSR;遗传多样性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31160388);; 广西自然科学基金重点项目(2013GXNSFDA019014);广西自然科学基金项目(2013GXNSFBA019112);; 广西科学研究与技术开发计划项目(桂科合1347004-11);; 国家现代农业(柑橘)产业技术体系建设专项(CARS-27-04B);; 桂林市科技开发项目(20120118-11);; 广西柑橘生物学重点实验室项目(桂柑科201301X004;桂柑科201202k001)

作者(Author): 刘冰浩;邓崇岭;陈传武;邓光宙;丁萍;牛英;唐艳;付慧敏;

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参考文献(References)：

• [1]GUO Tianchi,YE Yinmin.A new species of Citrus(rutaceae)from China[J].Acta Phytotaxonomica Sinica,1997,35(4):353-355.郭天池,叶荫民.中国柑橘属(芸香科)—新种[J].植物分类学报,1997,35(4):353-355.
• [2]DENG Chongling,XU Zhimei,DENG Guangzhou,LIU Binghao,TANG Yan,FU Huimin,SHI Jianquan,CHEN Chuanwu,NIU Ying.Wild Citrus resource exploration of Guposhan in Guangxi[J].South China Fruits,2013,42(5):9-11.邓崇岭,徐志美,邓光宙,刘冰浩,唐艳,付慧敏,石健泉,陈传武,牛英.广西贺州姑婆山野生柑橘资源的复核调查[J].中国南方果树,2013,42(5):9-11.
• [3]YAN Huaxue.Testing candidate plant barcode regions in the true citrus fruit trees group(Aurantioideae,Rutaceae)[D].Chongqing:Southwest University,2010.闫化学.柑橘及其近缘属植物DNA条形码研究[D].重庆:西南大学,2010.
• [4]LI Xiaomeng.Molecular phylogeny of the true citrus fruit trees group(Aurantioideae,Rutaceae)and the origin of cultivated Citrus[D].Chongqing:Southwest University,2010.李小孟.柑橘及其近缘属植物的分子进化与栽培柑橘的起源研究[D].重庆:西南大学,2010.
• [5]XIE Rangjin,ZHOU Zhiqin,DENG Lie.Taxonomic and phylogenetic relationships among the genera of the true Citrus fruit trees group(Aurantioideae,Rutaceae)based on AFLP markers[J].Journal of Systematics and Evolution,2008,46(5):682-691.谢让金,周志钦,邓烈.真正柑橘果树类植物基于AFLP分子标记的分类与进化研究[J].植物分类学报,2008,46(5):682-691.
• [6]DENG Chongling,LIU Binghao,CHEN Chuanwu,TANG Yan,DING Ping,FU Huimin,CHEN Rendian,SHI Jianquan.Analysis on life table of wild ichang papeda population in longsheng of Guangxi province[J].Journal of Fruit Science,2015,32(1):1-5.邓崇岭,刘冰浩,陈传武,唐艳,丁萍,付慧敏,陈仁电,石健泉.广西龙胜野生宜昌橙种群生命表分析[J].果树学报,2015,32(1):1-5.
• [7]LIANG Wujun,XIE Kaidong,GUO Dayong,XIE Zongzhou,YI Hualin,GUO Wenwu.Spontaneous generation and SSR molecular characterization of autotetraploids in ten Citrus rootstocks[J].Journal of Fruit Science,2014,31(1):1-6.梁武军,解凯东,郭大勇,谢宗周,伊华林,郭文武.10个柑橘砧木类型同源四倍体的发掘与SSR鉴定[J].果树学报,2014,31(1):1-6.
• [8]ZHANG Qi,AN Huaming.Genetic diversity of Pyrus pyrifolia resources distributed in Guizhou provence revealed by ISSR markers[J].Journal of Fruit Science,2015,32(1):6-12.张起,安华明.贵州砂梨种质资源遗传多样性的ISSR分析[J].果树学报,2015,32(1):6-12.
• [9]CHENG Yunjiang,YI Hualin,PANG Xiaoming,GUO Wenwu,DENG Xiuxin.An efficient method for genomic DNA extraction from woody fruit plants[J].Journal of Huazhong Agricultural University,2001,20(5):481-483.程运江,伊华林,庞晓明,郭文武,邓秀新.几种木本果树DNA的有效提取[J].华中农业大学学报,2001,20(5):481-483.
• [10]QIU Yingxiong,HU Shaoqing,CHEN Yuelei,CHEN Xiao,WU Guanghong.Studies on cultivar classification of Osmanthus fragrans by ISSR-PCR analysis[J].Acta Horticulture Sinica,2004,31(4):529-532.邱英雄,胡绍庆,陈跃磊,陈晓,吴光洪.ISSR-PCR技术在桂花品种分类研究中的应用[J].园艺学报,2004,31(4):529-532.
• [11]DONG Haiyan,JI Kongshu,HOU Boxin,ZHAO Hongbo.Genetic relatives analysis of 41 Loropetalum chinense var.rubrum cultivars by ISSR markers[J].Acta Horticulturae Sinica,2014,41(2):365-374.董海燕,季孔庶,侯伯鑫,赵宏波.基于ISSR标记的红花檵木品种亲缘关系分析[J].园艺学报,2014,41(2):365-374.
• [12]HE Xinhua,LI Yangrui,GUO Yongze,TANG Zhipeng,LI Rongbai.Genetic analysis of 23 mango cultivar collection in Guangxi province revealed by ISSR[J].Molecular Plant Breeding,2005,3(6):829-834.何新华,李杨瑞,郭永泽,唐志鹏,李容柏.23个广西本地芒果品种的ISSR分析[J].分子植物育种,2005,3(6):829-834.
• [13]SHI Yantong,ZHOU Bo,ZHANG Xiuxin,JIANG Haidong,XUE Jingqi,WANG Shunli.Assessment of genetic diversity and relationship of 89 tree Peony cultivars from different provenances[J].Acta Horticulturae Sinica,2012,39(12):2499-2506.石颜通,周波,张秀新,江海东,薛璟祺,王顺利.牡丹89个不同种源品种遗传多样性和亲缘关系分析[J].园艺学报,2012,39(12):2499-2506.
• [14]WU Xin,LEI Tiangang,HE Yongrui,LIU Xiaofeng,XU Lanzhen,PENG Aihong,CHEN Shanchun.Establishment and optimization of SRAP and ISSR marker system in Citrus[J].Molecular Plant Breeding,2008,6(1):170-176.吴鑫,雷天刚,何永睿,刘小丰,许兰珍,彭爱红,陈善春.柑橘SRAP和ISSR分子标记技术体系的建立与优化[J].分子植物育种,2008,6(1):170-176.
• [15]SONG Changmei,WEN Xiaopeng.Molecular evaluation of genetic diversity of cherry germplasm resource from Guizhou province[J].Guizhou Agricultural Sciences,2011,39(8):11-14.宋常美,文晓鹏.贵州樱桃种质资源遗传多样性的分子评价[J].贵州农业科学,2011,39(8):11-14.
• [16]ZHOU Kailong,YE Yinmin.China fruit records Citrus volume[M].Beiing:China Forestry Press,2009.周开隆,叶荫民.中国果树志柑橘卷[M].北京:中国林业出版社,2009.
• [17]LIAO Zhenkun,ZHANG Qiuming,LIU Weiguo,DING Weiping,ZHANG Ling.Identification of relative relationships of wild relatives of eucitrus originated from Nanling mountains by AFLP analysis[J].Journal of Hunan Agricultural University(Natural Sciences Edition),2006,3(4):385-388.廖振坤,张秋明,刘卫国,丁伟平,张玲.南岭山脉宽皮柑橘近缘野生种亲缘关系鉴定[J].湖南农业大学学报(自然科学版),2006,3(4):385-388.
• [18]LIU Yong,WU Bo,LIU Dechun,SUN Zhonghai.On genetic diversity of Jiangxi native Citrus and its wild varieties based on SSR markers[J].Acta Agriculturae Universitatis Jiangxiensis,2005,27(4):486-490.刘勇,吴波,刘德春,孙中海.江西柑橘地方品种资源及野生近缘种SSR分子标记[J].江西农业大学学报,2005,27(4):486-490.