甜瓜果实蔗糖代谢酶提取液中2种去糖方法的比较Comparison of Two De-sugaring Methods for Sucrose-Metabolizing Enzyme Extracting Solution of Muskmelon
乔永旭,刘栓桃,赵智中,邢国明,何启伟
摘要(Abstract)：
以甜瓜鲁厚甜1号开花当天的果实为试材,分别测定蔗糖代谢相关酶—酸性转化酶(AI)、中性转化酶(NI)、蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)的活性。对酶提取液中干扰测定结果的可溶性糖采取2种方法进行去除(1)直接用SephadexG-25柱离心去糖;(2)酶提取液先经过硫酸铵沉淀,去糖,重新溶解后再用SephadexG-25柱脱盐。结果显示,方法2处理后AI和NI、SPS和SS的活性均高于方法1。这表明在研究甜瓜果实蔗糖代谢相关酶活性时,采用方法2处理酶提取液效果更好。
关键词(KeyWords)： 甜瓜果实;酶提取液;去糖;酶活性
基金项目(Foundation): 山东省优秀中青年科学家科研奖励基金项目(鲁科计字[2001]342)。
作者(Author): 乔永旭,刘栓桃,赵智中,邢国明,何启伟
参考文献(References)：
- [1]WolosiukRW,PontisHG.Evidenceofthewxistenceoftwoformssucrosesynthase[J].FEBSlett,1971,16:237～240.
- [2]SungHY,SuJC.Sucrosesynthaseisozymesofpeaseedlings-purifi-cationandgeneralpropertises[J].JChinAgricChemSoc,1977,6:76～91.
- [3]HubbardNL,PharrDM,HuberSC.Sucrose-phosphatesynthaseandothersucrosemetabolizingenzymesinfruitsofvariousspecies[J].PhysiolPlant,1991,82:191～196.
- [4]NiesenTH,SkjarbakHC,KarlsenP.Carbohydratemetabolizingdur-ingfruitdevelopmentinsweetpepper(Capsicumannuum)plants[J].PhysiolPlant,1991,82:311～319.
- [5]TakayaMoriguchi,Kazuyuki,TetsuroSanada,etal.Levesandroleofsucrosesynthase,sucrosephosphatesynthase,andacidinvertaseinsucroseaccumulationinfruitofAsianpear[J].JAmerSocHortSci,1992,117(2):274～278.
- [6]McCollumTG,HuberDJ,CantliffeDJ.Solulesugaraccumulationandactivityofrelatedenzymesduringmuskmelonfruitdevelopment[J].JAmerSocHortSci,1988,113(3):399～403.
- [7]赵智中,张上隆,徐昌杰,等.蔗糖代谢相关酶在温州蜜柑果实糖积累中的作用[J].园艺学报,2001,28(2):112～118.
- [8]HelmerhorstE,StokesGB.Microcentrifugedesalting:Arapidquan-titativefordesaltingsmallamountofprotein[J].AnalBiochem,1980,104:130～135.
- [9]LowellCA,TomlinsonPT,KochKE.Sucrose-metabolizingenzymesintransporttissueandadjacentsinkstructuresindevelopingcitrusfruit[J].PlantPhysiol,1989,90:1394～1402.
- [10]KomatsuA,TakanokuraY,MoriguchiT,etal.Differentialexpres-sionofthreesucrose-phosphatesynthaseisoformsduringaccumula-tionincitrusfruit(CitrusunshiuMarc.)[J].PlantSci,1999,140:169～178.
- [11]严慧敏,黄炜,廖兆全,等.超声波和TritonX100对鸟氨酸氨基甲酰转移酶催化活性的影响[J].催化学报,1999,20(4):479.
- [12]王培昌,吴逸明,赵洛沙,等.巯基乙醇和维生素C对镉孵育红细胞SOD活性的影响[J].中国职业医学,2000,27(4):15～16.