湖北海棠种内遗传变异的SRAP分析The intraspecific variation of Malus hupehensis analyzed by SRAP
郭大勇;徐育海;张靖国;罗正荣;
摘要(Abstract):
应用12对引物对33份湖北海棠样本进行了SRAP分析,每对引物产生4~13条谱带,多态性条带占总带数的91.225%;33个基因型的相似系数在0.5104~0.9271,表明湖北海棠种下具有较丰富的遗传多样性。应用UPGMA法聚类分析表明,在L=0.69时,33份供试材料分为5个组,在L=0.63时,分为3个组。主坐标分析显示,33种供试材料分为5个组,除一份材料外,其余材料分组与聚类分析L=0.69时分组情况相同。在聚类的同一组里,有不同来源地的湖北海棠样品,而在同一来源地的湖北海棠又有的不能聚在同一组,主坐标分析亦获得基本相同的结果,表明湖北海棠的遗传多样性与分布地域间没有对应关系,平邑甜茶或起源于神农架地区。部分材料相似系数较大,有几对甚至难以区分,而兴山35号却与其他类型差别明显大。
关键词(KeyWords): 湖北海棠;SRAP;亲缘关系
基金项目(Foundation):
作者(Author): 郭大勇;徐育海;张靖国;罗正荣;
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参考文献(References):
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