孙敏红;卢晓鹏;曹雄军;李静;熊江;谢深喜;

• 1:湖南农业大学园艺园林学院
• 2:中南林业科技大学林学院

摘要(Abstract)：

【目的】进一步探讨枳橙对氮素吸收利用机制,为柑橘生产上提高氮素利用效率和科学施肥提供科学依据。【方法】以枳橙为材料,采用水培方法,在Hoagland配方的基础上进行调节,研究了不同形态氮素配比对枳橙幼苗硝酸还原酶(NR)活性变化及相关基因表达量的影响。【结果】幼苗叶片及根系NR活性均随着培养时间的延长而增加,且叶片NR活性高于根系。其中2号(NO_3~-∶NH_4~+=7∶3)和3号(NO_3~-∶NH_4~+=5∶5)处理的叶片酶活最高;1号(NO_3~-∶NH_4~+=10∶0)和2号(NO_3~-∶NH_4~+=7∶3)处理根系的NR活性显著高于其他处理;进一步研究NR相关基因表达情况表明:3个基因(CitNia1、CitNia2、CitNia OA)在不同处理的枳橙幼苗体内均有表达,其中CitNia1和CitNia2分别是根系和叶片NR相关主效基因,2者均受到NO_3~-、NH_4~+配比诱导,且3号处理(NO_3~-∶NH_4~+=5∶5)下表达量最高。【结论】枳橙幼苗硝态氮代谢的主要部位为叶片,且CitNia2是主效基因,通过调节铵硝比例可以促进其表达。

关键词(KeyWords)： 枳橙;硝态氮;铵态氮;硝酸还原酶

Abstract:

Keywords:

基金项目(Foundation): 现代农业(柑橘)产业技术体系(CARS-027);; 国家自然科学基金(31401824);; 教育部高校博士点基金(20134320120006)

作者(Author): 孙敏红;卢晓鹏;曹雄军;李静;熊江;谢深喜;

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