不同氮素形态培养对枳橙幼苗硝酸还原酶活性及相关基因表达的影响Effect of different forms of nitrogen on the activity of nitrate reductase and expression of the relative genes in Citrus sinensis × Poncirus trifoliate
孙敏红;卢晓鹏;曹雄军;李静;熊江;谢深喜;
摘要(Abstract):
【目的】进一步探讨枳橙对氮素吸收利用机制,为柑橘生产上提高氮素利用效率和科学施肥提供科学依据。【方法】以枳橙为材料,采用水培方法,在Hoagland配方的基础上进行调节,研究了不同形态氮素配比对枳橙幼苗硝酸还原酶(NR)活性变化及相关基因表达量的影响。【结果】幼苗叶片及根系NR活性均随着培养时间的延长而增加,且叶片NR活性高于根系。其中2号(NO_3~-∶NH_4~+=7∶3)和3号(NO_3~-∶NH_4~+=5∶5)处理的叶片酶活最高;1号(NO_3~-∶NH_4~+=10∶0)和2号(NO_3~-∶NH_4~+=7∶3)处理根系的NR活性显著高于其他处理;进一步研究NR相关基因表达情况表明:3个基因(CitNia1、CitNia2、CitNia OA)在不同处理的枳橙幼苗体内均有表达,其中CitNia1和CitNia2分别是根系和叶片NR相关主效基因,2者均受到NO_3~-、NH_4~+配比诱导,且3号处理(NO_3~-∶NH_4~+=5∶5)下表达量最高。【结论】枳橙幼苗硝态氮代谢的主要部位为叶片,且CitNia2是主效基因,通过调节铵硝比例可以促进其表达。
关键词(KeyWords): 枳橙;硝态氮;铵态氮;硝酸还原酶
基金项目(Foundation): 现代农业(柑橘)产业技术体系(CARS-027);; 国家自然科学基金(31401824);; 教育部高校博士点基金(20134320120006)
作者(Author): 孙敏红;卢晓鹏;曹雄军;李静;熊江;谢深喜;
Email:
DOI: 10.13925/j.cnki.gsxb.20160314
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