薛华柏;杨健;王龙;王苏珂;张慧蓉;乔玉山;章镇;李秀根;

• 1:中国农业科学院郑州果树研究所
• 2:南京农业大学园艺学院

摘要(Abstract)：

【目的】为便于果树种质区分鉴定,资源交换、管理和利用,新品种特异性、一致性和稳定性(DUS)测试,以29个梨品种SSR指纹数据作为基础数据,演示了一种二倍体果树品种SSR特征指纹数据表的构建方法。【方法】经过SSR位点基因型数目统计,SSR位点排序,供试品种排序区分和数据标示,表格优化等数据整合处理步骤,构建了29个梨品种SSR特征指纹数据表。【结果】12对SSR引物中,CH04h02和NAUpy65d 2对引物能将供试的29个梨品种全部区分开,可作为鉴定这29个梨品种时的核心引物加以利用。【结论】该方法简便易行,特征指纹数据表数据信息展示全面直观,品种区分关系一目了然,便于交流共享和指纹图谱数据库共建。

关键词(KeyWords)： 梨;分子标记;SSR;品种鉴定;指纹数据表

Abstract:

Keywords:

基金项目(Foundation): 现代农业(梨)产业技术体系(CARS-29);; 国家高技术研究发展(863)计划(2011AA10020604,2013AA102606-2)

作者(Author): 薛华柏;杨健;王龙;王苏珂;张慧蓉;乔玉山;章镇;李秀根;

Email:

DOI: 10.13925/j.cnki.gsxb.20140456

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