龙眼水通道蛋白基因(DLPIP1)的克隆与表达分析Cloning and expression of longan aquaporin(DLPIP1)gene
陈虎;何新华;罗聪;邓立宝;胡颖;李明娟;杨丽涛;
摘要(Abstract)：
应用蛋白质组学研究低温胁迫下龙眼叶片蛋白质组变化时,发现PIP1蛋白在龙眼低温胁迫中上调表达。应用RACE技术克隆龙眼水通道蛋白基因全长cDNA,命名为DLPIP1,基因登陆号为JN572691,长度为1 132 bp,包括1个900 bp的开放阅读框,编码299个氨基酸序列,同源性分析表明,DLPIP1在21个不同植物中的一致性为90%~93%。应用生物信息学软件对DLPIP1氨基酸序列分析表明,含有7个跨膜区,有2个NPA单元,其氨基酸残基与MIP家族蛋白保守区序列完全一致。氨基酸序列比对发现,该序列与其他物种PIP质膜水通道蛋白氨基酸序列有很高的同源性。利用实时荧光定量技术对DLPIP1在低温胁迫下不同组织表达谱分析表明,DLPIP1在龙眼根、茎、叶中都有表达,在根中的表达量最高,其次是茎和叶。DLPIP1在低温胁迫时,随着低温胁迫时间的延长而发生变化。这说明DLPIP1蛋白在龙眼低温逆境过程中起作用。
关键词(KeyWords)： 龙眼;水通道蛋白;低温胁迫;克隆;表达
基金项目(Foundation): 国家科技支撑计划项目(2008BADB8B01,2008BADB8B02);; 广西自然科学基金项目(2011GXNSFA018115);; 广西研究生创新计划项目(GXU11T31077);; 广西高等学校优秀人才资助计划项目(桂教人201065)
作者(Author): 陈虎;何新华;罗聪;邓立宝;胡颖;李明娟;杨丽涛;
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参考文献(References)：
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