赵欢;王玉凯;牛建新;

• 1:石河子大学农学院园艺系·特色果蔬栽培生理与种质资源利用兵团重点实验室

摘要(Abstract)：

【目的】明确'库尔勒香梨'kfpMYB基因表达高低与萼片脱落和宿存的相关性。【方法】在新疆库尔勒巴格吉格代村梨园于盛花期选取强势树和弱势树各3株,各采30朵花的萼片,共180朵。同时每株树上各标记100朵花,落花7 d后进行调查,记录萼片的宿存与脱落情况。参照艾德莱植物RNA快速提取试剂盒的说明书提取总RNA。以总RNA为模板,按照TakaRa PrimescriptTMRT reagent Kit试剂盒的说明书合成'库尔勒香梨'cDNA第一条链。根据cDNA序列信息设计引物,通过实时荧光定量PCR明确kfpMYB基因在强势树与弱势树中的相对表达量,并用SPSS软件分析田间调查的数据。【结果】kfpMYB基因在强势树2～5序位萼片中的表达量均高于弱势树。kfpMYB基因在强势树和弱势树第2序位的表达量显著高于其他序位的表达量。强势树和弱势树的第2、3序位的宿萼率均显著高于第4、5序位,第5序位的脱萼率显著高于其他序位。不同树势中'库尔勒香梨'萼片kfpMYB基因相对表达量高低与萼片脱落和宿存有相关性,但不显著;在不同序位中,第4序位中'库尔勒香梨'萼片kfpMYB基因的相对表达量与萼片宿存呈显著负相关,第5序位中'库尔勒香梨'萼片kfpMYB基因的相对表达量与萼片脱落呈显著负相关。【结论】'库尔勒香梨'萼片kfpMYB基因的表达量与萼片的脱落和宿存存在相关性,不同树势中的相关性不显著,但在不同序位中存在显著相关。

关键词(KeyWords)： '库尔勒香梨';萼片;kfpMYB;相关性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31360474);; 高等学校博士学科点专项科研基金博导类联合资助课题(2013651810002)

作者(Author): 赵欢;王玉凯;牛建新;

Email:

DOI: 10.13925/j.cnki.gsxb.20170504

参考文献(References)：

• [1]何子顺,牛建新,吴忠华,覃伟铭,赵建设.库尔勒香梨花萼发育规律研究[J].新疆农业科学,2007,44(3):377-381.HE Zishun,NIU Jianxin,WU Zhonghua,QIN Weiming,ZHAOJianshe.Research of the development of Korla pear calyx[J].Xinjiang Agricultural Sciences,2007,44(3):337-381.
• [2]何子顺,李世强,茹仙古丽,张峰,牛建新.库尔勒香梨外观品质影响因素研究[J].中国果树,2010(6):16-18.HE Zishun,LI Shiqiang,RU Xianguli,ZHANG Feng,NIU Jianxin.Study on the factors affecting the appearance quality of Korla fragrant pear[J].China Fruits,2010(6):16-18.
• [3]李林,何晓霞.植物生长调节剂对库尔勒香梨脱落与宿存调控的研究[J].新疆林业,2008(1):29-30.LI Lin,HE Xiaoxia.Study on the regulation of the abscission and storage of Korla Fragrant Pear by plant growth regulator[J].Forestry of Xinjiang,2008(1):29-30.
• [4]井春芝,王允栋.库尔勒香梨矮化中间砧的筛选试验初报[J].北方果树,2002(1):13.JING Chunzhi,WANG Yundong.Preliminary report on the screening test of Korla pear dwarf intermediate rootstock[J].Northern Fruits,2002(1):13.
• [5]刘妮,陶书田,张绍铃,曹玉芬,吴华清,王继忠,吴涛.授粉品种对库尔勒香梨果实萼片宿存及品质的影响[J].南京农业大学学报,2011,34(3):43-47.LIU Ni,TAO Shutian,ZHANG Shaoling,CAO Yufen,WUHuaqing,WANG Jizhong,WU Tao.Effects of pollinated varieties on the storage and quality of calyx of Korla pear[J].Journal of Nanjing Agricultural University,2011,34(3):43-47.
• [6]邵月霞,牛建新,何子顺.影响库尔勒香梨果实脱萼宿萼因素研究[J].西北园艺,2007(4):39-40.SHAO Yuexiao,NIU Jianxin,HE Zishun.Study on the factors affecting the dealyx calyx of Korla pear fruit[J].Northwest Horticulture,2007(4):39-40.
• [7]亚合甫·木沙,牛建新,席万鹏.影响库尔勒香梨宿萼果率因素的调查[J].中国果树,2007(4):13-15.YA Hepu musha,NIU Jianxin,XI Wanpeng.Investigation on the factors affecting the rate of calyx fruit in Korla pear[J].China Fruits,2007(4):13-15.
• [8]QI X X,WU J,WANG L F,LI L L,CAO Y F,TIAN L M,DONG X G,ZHANG S L.Identifying the candidate genes involved in the calyx abscission process of‘Kuerlexiangli’(Pyrus sinkiangensis Yu)by digital transcript abundance measurements[J].BMC Genomics,2013,14(1):727.
• [9]牛建新,何子顺.梨果萼脱落宿存过程中果萼幼果内源激素的变化动态[J].果树学报,2009,26(4):431-434.NIU Jianxin,HE Zishun.The dynamic changes of pear calyx fruit in the process of endogenous hormones[J].Journal of Fruit Science,2009,26(4):431-434.
• [10]张飞,牛建新,叶春秀,刘娜,高静涛,朱天丁.库尔勒香梨mRNA差异显示技术体系建立[J].石河子大学学报(自然科学版),2011,29(2):173-178.ZHANG Fei,NIU Jianxin,YE Chunxiu,LIU Na,GAO Jingtao,ZHU Tianding.Establishment of mRNA differential display technology system for Korla fragrant pear[J].Journal of Shihezi University(Natural Science Edition),2011,29(2):173-178.
• [11]董芳园,张飞,王钰婷,牛建新.库尔勒香梨萼片脱落与宿存相关基因的差异表达分析[J].新疆农业科学,2013,50(1):57-64.DONG Fangyuan,ZHANG Fei,WANG Yuting,NIU Jianxin.Differential expression analysis of calyx falling off Korla fragrant pears and their pesistent related genes[J].Xinjiang Agricultural Sciences,2013,50(1):57-64.
• [12]WANG B H,SUN X X,DONG F Y,ZHANG F,NIU J X.Cloning and expression analysis of an MYB gene associated with calyx persistence in Korla Fragrant pear[J].Plant Cell Reports,2014,33(8):1333-1341.
• [13]王博慧,孙晓霞,牛建新.库尔勒香梨kfpMYB及其启动子的克隆和对激素的应答反应[J].园艺学报,2015,32(8):1448-1456.WANG Bohui,SUN Xiaoxia,NIU Jianxin.Cloning of kfpMYBgene and its promoter in Korla Fragrant Pear(Pyrussinkiangensis)and determination their responses to hormones[J].Acta Horticulturae Sinica,2015,32(8):1448-1456.
• [14]孙晓霞,牛建新,王博慧,裴茂松,李陈静,曹福军.‘库尔勒香梨’脱萼组与宿萼组样品差异表达基因的筛选[J].果树学报,2015,32(6):1020-1027.SUN Xiaoxia,NIU Jianxin,WANG Bohui,PEI Maosong,LIChenjing,CAO Fujun.Screening of differentially expressed genes in‘Korla fragrant pear’flower samples with persistent or deciduous calyx[J].Journal of fruit science,2015,32(6):1020-1027.
• [15]吴春霞.植物MYB基因研究进展[J].安徽农业科学,2009,37(20):9372-9374.WU Chunxia.Progress in the study of plant MYB gene[J].Journal of Anhui Agricultural Sciences,2009,37(20):9372-9374.
• [16]杜海,杨文杰,刘蕾,唐晓凤,吴燕民,黄玉碧,唐益雄.大豆MYB转录因子基因GmMYBJ6和GmMYBJ7的克隆及表达分析[J].作物学报,2008,34(7):1179-1187.DU Hai,YANG Wenjie,LIU Lei,TANG Xiaofeng,WU Yanmin,HUANG Yubi,TANG Yixiong.Cloning and functional identification of the two MYB transcription factors GmMYBJ6and GmMYBJ7 in soybean[J].Acta Agronomica Sinica,2008,34(7):1179-1187.
• [17]BAUMANN K,PEREZ R M,BRADLEY D,VENAIL J,BAI-LEY P,JIN H,KOES R,ROBERTS K,MARTIN C.Control of cell and petal morphogenesis by R2R3 MYB transcription factors[J].Development,2007,134(9):1691-1701.
• [18]JAKOBY M J,FALKENHAN D,MADER M,BRININSTOOLG,WISCHNITZKI E,PLATZ N,HUDSON A,Hülskamp M,Larkin J,Schnittger A.Transcriptional profiling of mature Arabidopsis trichomes reveals that NOECK encodes the MIXTA-like transcriptional regulator MYB106[J].Plant Physiology,2008,148(3):1583-1602.
• [19]ZHANG Y,CAO G,QU L J,GU H.Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15[J].Journal Genet Genomics,2009,36(2):99-107.
• [20]LAI L B,NADEAU J A,LUCAS J,LEE E K,NAKAGAWA T,ZHAO L,GEISLER M,SACK F D.The Arabidopsis R2R3MYB proteins FOUR LIPS and MYB88 restrict divisions late in the stomatal cell lineage[J].Plant Cell,2005,17(10):2754-2767.
• [21]XIE Z,LEE E,LUCAS J R,MOROHASHI K,LI D,MURRAYJ A,SACK F D,GROTEWOLD E.Regulation of cell proliferation in the stomatal lineage by the Arabidopsis MYB FOURLIPS via direct targeting of core cell cycle genes[J].Plant Cell,2010,22(7):2306-2321.
• [22]DING Z H,LI S M,AN X L.Transgenic expression of MYB15confers enhanced sensitivity to abacisic acid and improved drought tolerance in Arabidopsis thaliana[J].Journal of Genetics and Genomics,2009,26:17-29.
• [23]徐庆帕,覃伟铭,李春江,翟林涛,魁丹.库尔勒香梨产生粗皮果的原因分析[J].落叶果树,2002(1):59.XU Qingpa,QIN Weiming,LI Chunjiang,ZHAI Lintao,KUIDan.Analysis of the causes of producing roughbark fruit of Korla fragrant pear[J].Deciduous fruit tree,2002(1):59.
• [24]邵月霞,牛建新,何子顺.库尔勒香梨果实脱萼与宿萼的研究概述[J].现代农业科技,2007(10):46-49.SHAO Yuexia,NIU Jianxin,HE Zishun.A review of the studies on calyx and calyx of Korla pear fruit[J].Modern agricultural science and technology,2007(10):46-49.
• [25]韩碧文.植物生长与分化[M].北京:中国农业大学出版社,2003:28.HAN Biwen.Plant growth and differentiation[M].Beijing:China Agricultural University press,2003:28.
• [26]张大海.库尔勒香梨果形变化规律研究[J].新疆农业科学,1999,36(4):261-263.ZHANG Dahai.Study on the change of fruit shape of Korla pear[J].Xinjiang Agricultural Sciences,1999,36(4):261-263.
• [27]马宏超.库尔勒香梨果实脱萼宿萼和粗皮果的显微结构及品质研究[D].乌鲁木齐:新疆农业大学,2010.MA Hongchao.Study on the microstructure and quality of Korla pear fruit calyx and persistent calyx roughbark pear[D].Urumchi:Xinjiang Agricultural University,2010.
• [28]任莹莹,李疆,覃伟铭,郭秋智.库尔勒香梨萼片脱落与宿存特性极其调控的初步研究[J].新疆农业大学学报,2007,30(1):25-29.REN Yingying,LI Jiang,QIN Weiming,GUO Qiuzhi.Preliminary studies on characteristic and control of calyx leaving and persistent from fruit of Korla Fragrant Pear[J].Journal of Xinjiang Agricultural University,2007,30(1):25-29.
• [29]陈昭存,葛敏,吴邦良,陈曾逸.果树调节剂PBO在砀山酥梨上的应用[J].果农之友,2003(7):15-16.CHEN Shaocun,GE Min,WU Bangliang,CHEN Zengyi.Application of fruit tree in Dangshan pear on the PBO regulator[J].Fruit Growers’Friend,2003(7):15-16.
• [30]玉山·库尔班,木合塔尔·扎热,齐曼·尤努斯,覃伟铭.复合试剂“M”和PBO对库尔勒香梨产量和果实品质的影响[J].新疆农业科学,2017,54(4):1-7.YU Shan KU Erban,NU Hedaer ZHA Re,QI Man YOU Nusi,QIN Weiming.Effect of compound reagent“M”and PBO on the yield and fruit quality of Korla pear[J].Xinjiang Agricultural Sciences,2017,54(4):1-7.