张规富;卢晓鹏;谢深喜;

• 1:湖南农业大学园艺园林学院
• 2:湖北科技学院核技术与化学生物学院

摘要(Abstract)：

【目的】探究不同时期水分胁迫处理对椪柑果实有机酸含量及与柠檬酸代谢相关酶基因表达的影响。【方法】以6 a(年)生盆栽枳砧普通椪柑(Citrus reticulata Blanco)为试材,设全期、前中期、前期、中期、后期等5个处理,以正常浇水为对照。用HPLC测定果肉中酸的含量,用相对荧光定量PCR测定柠檬酸代谢相关基因的相对表达量。【结果】5个处理组均导致果实中柠檬酸、苹果酸、奎宁酸等3种有机酸及总酸浓度显著增加;Cit CS1与Cit CS2的相对表达量均出现不同程度上调;Cit Aco1的相对表达量总体是上调表达,Cit Aco2的相对表达量因不同时期处理而异,Cit Aco3的相对表达量出现不同程度下降;Cit IDH2在3个Cit IDH基因中相对表达量最低,但其表达均得到促进,Cit IDH1的相对表达量因不同时期而异,而对Cit IDH3而言,其相对表达量总体是抑制表达;2个Cit GAD基因中,Cit GAD5启动较早,水分胁迫下Cit GAD4、Cit GAD5的表达均受到抑制,Cit GAD5所受影响更为显著。【结论】不同时期水分胁迫能导致椪柑果实柠檬酸积累,总有机酸含量升高,这种变化与柠檬酸代谢相关基因表达量发生变动有关。其中,Cit CS1、Cit CS2表达上调及Cit Aco3、Cit IDH3、Cit GAD4和Cit GAD5表达下调可能是果实柠檬酸积累的原因之一。

关键词(KeyWords)： 椪柑;水分胁迫;果实;柠檬酸;基因表达量

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业(柑橘)产业体系项目(CARS);; 国家自然科学基金(31071763);; 湖南省科技计划项目(2014NK3003)

作者(Author): 张规富;卢晓鹏;谢深喜;

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