岳海梅;庄华;巩文峰;张新军;

• 1:西藏农牧学院植物科学学院
• 2:中国农业大学植物保护学院
• 3:西北农林科技大学植物保护学院·旱区作物逆境生物学国家重点实验室
• 4:西藏高原生态研究所

摘要(Abstract)：

【目的】筛选适合于防治苹果褐腐病(Monilinia fructigena)的生防毛壳菌,为苹果采后病害的生物防治提供潜在的菌株材料。【方法】选择4株毛壳菌,采用平板对峙法测量抑菌率和抑菌带宽度;通过生防毛壳菌发酵粗提物检测其抑菌活性强弱;通过离体接种苹果果实比较毛壳菌的控病作用。结合形态学特征和核糖体基因内转录间隔区(r DNA-ITS)序列对抑菌活性强的毛壳菌菌株进行鉴定。【结果】供试的4株毛壳菌中,菌株24-9在平板对峙试验、发酵粗提物抑菌试验和离体接种试验中均表现出稳定而较强的抑菌活性,通过形态学特征和核糖体基因内转录间隔区(rDNA-ITS)序列构建系统发育树,将菌株24-9鉴定为球毛壳菌Chaetomium globosum。【结论】该生防毛壳菌菌株可用于开发苹果采后病害生防制剂,提高植物抗性,丰富我国苹果采后病害的生防菌资源库。

关键词(KeyWords)： 苹果褐腐病;生防毛壳菌;筛选;鉴定

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31360006);; 旱区作物逆境生物学国家重点实验室开放基金课题(CSBAA2016006)

作者(Authors): 岳海梅;庄华;巩文峰;张新军;

DOI: 10.13925/j.cnki.gsxb.20160422

参考文献(References)：

• [1]BARRITT B H.The apple world 2003-present situation and developments for produces and consumers[J].The Compact Fruit Tree,2003,36(1):15-18.
• [2]徐海晶,于冷.我国苹果出口贸易的实证分析[J].农业技术经济,2006(2):43-47.XU Haijing,YU Leng.Analysis on the import and export of China’s apple trade[J].Journal of Agrotechnical Economics,2006(2):43-47.
• [3]崔挺,杨文杰.入世五年中国苹果贸易竞争力国际比较[J].西北林学院学报,2008,23(4):223-227.CUI Ting,YANG Wenjie.The comparion of competitiveness of Chinese apple trade since China’s accession to the WTO[J].Journal of Northwest Forestry University,2008,23(4):223-227.
• [4]李丽,刘涛,倪新,刘彩虹,王跃进,王勇,伦才智.苹果褐腐病采后丙酸热水联合处理技术研究[J].食品科技,2016,41(6):8-32.LI Li,LIU Tao,NI Xin,LIU Caihong,WANG Yuejin,WANG Yong,LUN Caizhi.The postharvest treatment of apple brown rot disease with combined propanoic acid and hot water[J].Food Science and Techonology,Echnolog,2016,41(6):8-32.
• [5]李侠,王跟波.苹果褐腐病防治要点[J].西北园艺,2007(4):48-49.LI Xia,WANG Genbo.Prevention and control of Monilinia fructigena[J].Northwest Horticulture,2007(4):48-49.
• [6]李凯,袁鹤.植物病害生物防治概述[J].山西农业科学,2012,40(7):807-810.LI Kai,YUAN He.Review on biological control of plant disease[J].Journal of Shanxi Agricultural Sciences,2012,40(7):807-810.
• [7]李海燕,张显龙,关丽杰,邵双,马金姗.苹果褐腐病菌中草药抑菌剂的筛选及提取条件研究[J].安徽农业科学,2009,37(10):4538-4540.LI Haiyan,ZHANG Xianlong,GUAN Lijie,SHAO Shuang,MA Jinshan.Research on screening Chinese herbal fungicides for Monilia fructigena and their extraction conditions[J].Journal of Anhui Agriculture Science,2009,37(10):4538-4540.
• [8]郭松年,井泽良,孙海燕,刘兴华.丁香提取物对三种果实致腐真菌的抑制作用研究[J].食品研究与开发,2006,27(3):160-161.GUO Songnian,JING Zeliang,SUN Haiyan,LIU Xinghua.The study of effect of extracts from clove on inhibition against 3 rotcauseing fungi in postharvest fruit[J].Food Research and Development,2006,27(3):160-161.
• [9]李鹏霞,邵世达,冯俊涛,蒲占胥,张兴.丁香精油和丁香酚对苹果贮藏期病害及果实品质的影响[J].农业工程学报,2006,22(6):173-177.LI Pengxia,SHAO Shida,FENG Juntao,PU Zhanxu,ZHANG Xing.Effects of essential oil and phenolic extracted from clove leaves(Syringa portolaciniata)on postharvest apple pathogens and quality[J].Transactions of the CSAE,2006,22(6):173-177.
• [10]刘守安,李多川,俄世瑾,张燕.嗜热毛壳菌纤维素酶(CBHⅡ)c DNA的克隆及在毕赤酵母中的表达[J].生物工程学报,2005,21(6):892-899.LIU Shouan,LI Duochuan,E Shijin,ZHANG Yan.Cloning and expressing of cellulase gene(CBHⅡ)from thermophilic fungi Chaetomium thermophilum CT2[J].Chinese Journal of Biotechnology,2005,21(6):892-899.
• [11]张海燕,杨谦.角毛壳菌生物防治相关基因的筛选[J].中国生物防治,2007,23(2):191-194.ZHANG Haiyan,YANG Qian.Selection of biocontrol-related genes from Chaetomium cupreum[J].Chinese Journal of Biological Control,2007,23(2):191-194.
• [12]辛雅芬,米士伟,张淑玲,邓建玲,高克祥.球毛壳菌在黄瓜上的侵染定殖及其对黄瓜生长和产量的影响[J].吉林农业大学学报,2013,35(4):450-456.XIN Yafen,MI Shiwei,ZHANG Shuling,DENG Jianling,GAO Kexiang.Infection and colonization and its effect on growth of Globosum ND35 in cucumber and yield of cucumber[J].Journal of Jilin Agricultural University,2013,35(4):450-456.
• [13]印敬明,刘晓光,万慧,肖守华,高克祥,王庆华.螺旋毛壳Chaetomium spirale ND35防病促生作用初探[J].莱阳农学院学报,2006,23(4):272-275.YIN Jingming,LIU Xiaoguang,WAN Hui,XIAO Shouhua,GAO Kexiang,WANG Qinghua.A preliminary investigation on biocontrol and growth promotion by endophytic Chaetomium spirale ND35[J].Journal of Laiyang Agricultural College,2006,23(4):272-275.
• [14]SOYTONG K,KANOLMEDHAKU S,KULONGVIRIYAPA V.Application of Chaetomium species(Ketomitum)as a new broad spectrum biological fungicide for plant disease control:A review article[J].Fungal Diversity,2001,7:1-15.
• [15]BI Y,TIAN S P,GUO Y R,GE Y H,QIN G Z.Sodium silicate reduces postharvest decay on Hami melons:induced resistance and fungistatic effects[J].Plant Disease,2006,90(3):279-283.
• [16]ARX J A,VON G J,FIGUERAS M J.The ascomycete genus Chaetomium[M].Incorporated:Gebruder Borntraeger Verlagsbuchhandlung Publisher Balogh International,1986,84:1-162.
• [17]甘丽萍,王生荣.RAPD标记在植物病原真菌遗传多样性及生理分化研究中的应用[J].甘肃农业大学学报,2004,40(1):35-40.GAN Liping,WANG Shengrong.Application of RAPD in the studies on genetic diversity and physiological specialization of plant pathogenic fungi[J].Journal of Gansu Agricultural University,2004,40(1):35-40.
• [18]王春华,杨芳雪.绿色贸易壁垒对山东苹果出日的影响及对策[J].山东农业科学,2015,47(12):143-146.WANG Chunhua,YANG Fangxue.Influences of green trade of Shandong province barrier to apple export and countermeasures[J].Shandong Agricultural Sciences,2015,47(12):143-146.
• [19]刘英,赵行,魏欣荣,胡作栋.苹果褐腐病的发生规律和防治技术[J].西北园艺:果树专刊,2010(5):27-28.LIU Ying,ZHAO Xing,WEI Xinrong,HU Zuodong.Occurrence and control techniques of Monilinia fructigena[J].Northwest Horticulture:Fruit trees,2010(5):27-28.
• [20]崔家升,李晓萍.世界苹果种植概况与我国苹果生产前景展望[J].北方果树,2012(4):1-3.CUI Jiasheng,LI Xiaoping.World apple planting and China apple production prospect[J].Northern Fruits,2012(4):1-3.