肖翠,严佳文,龙桂友,戴素明,李大志,邓子牛

• 1:湖南农业大学园艺园林学院·国家柑橘改良中心长沙分中心

摘要(Abstract)：

【目的】为了筛选在柑橘中稳定表达的内参基因,以确保柑橘基因表达分析结果的可靠性。【方法】选取Actin、18SrRNA、rpII、GAPDH、UBQ10、UBQ1作为候选内参基因,以geNorm、Normfinder、BestKeeper以及RefFinder软件分析这6个基因在冰糖橙的根、茎、叶、果实以及干旱、低温、溃疡病、衰退病胁迫下叶片中的表达稳定性。【结果】结果表明,在不同柑橘器官及不同胁迫处理下的柑橘叶片中,候选内参基因的表达稳定性确实存在差异。在不同柑橘器官之间比较用UBQ10和rpII;干旱胁迫下用GAPDH、18SrRNA和Actin;低温胁迫下用UBQ10、Actin和18Sr-RNA;溃疡病菌侵染胁迫下用Actin、UBQ10和GAPDH;感染衰退病毒时用18SrRNA和rpII。【结论】为了获得较为稳定的表达分析结果,建议根据不同的试验内容选择不同的内参基因组合。

关键词(KeyWords)： 柑橘;内参基因;稳定性评价;RT-qPCR;基因表达分析

Abstract:

Keywords:

基金项目(Foundation): 国家国际科技合作专项(2011DFA32030);; 柑橘产业技术体系(CARS-27);; 湖南省科学技术厅科技计划重点项目(2011WK2007)

作者(Author): 肖翠,严佳文,龙桂友,戴素明,李大志,邓子牛

DOI: 10.13925/j.cnki.gsxb.2012.06.010

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