张雪;唐铭浩;陈蒙;李春艳;刘海峰;

• 1:延边大学农学院

摘要(Abstract)：

【目的】探究山葡萄果皮转色过程中相关基因的表达。【方法】以不同成熟期的山葡萄果皮为材料,采用高通量测序技术进行转录组分析,探讨其转色过程中花色苷含量及其相关基因的变化。【结果】经过测序得到3个时期的clean reads分别为:转色前20 157 930条,50%着色期16 197 432条,完熟期16 410 824条;GC含量分别为48.41%、48.04%和49.54%;各时期的Q30大于等于94.73%。通过与参考基因组进行序列比对,各样品Read与参考基因组的比对效率为60.57%⁶7.77%,并且唯一比对位置的数量均超过58.99%,比对到基因组上的序列绝大部分分布在外显子区,均为96.3%以上。本试验将3个样品中转色前分别与50%成熟期和完熟期进行了基因差异表达统计分析,结果显示COG功能注释分别为1 305、1 602个。【结论】明确了山葡萄果皮转色过程中相关基因的变化,为进一步大量挖掘山葡萄果皮成熟过程中重要表达基因奠定一定的基础。

关键词(KeyWords)： 山葡萄;转录组;测序;功能注释

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31260067);; 吉教科合字[2016]第255号

作者(Authors): 张雪;唐铭浩;陈蒙;李春艳;刘海峰;

DOI: 10.13925/j.cnki.gsxb.20160404

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