程远;万东艳;张宝林;王跃进;文颖强;

• 1:西北农林科技大学园艺学院·农业部西北地区园艺作物生物学与种质创新重点实验室·旱区作物逆境生物学国家重点实验室

摘要(Abstract)：

【目的】通过诱导、保存和扩繁葡萄原胚团,为葡萄外源基因转化及基因编辑研究提供受体材料。【方法】以4个欧亚种葡萄品种和1个野生种葡萄的花器官为材料,比较不同外植体在3种培养基上胚性愈伤组织的诱导率,通过二次再生途径诱导、保存和扩繁次生原胚团并利用改良苯酚品红染色法对其进行细胞学观察。【结果】‘无核白’葡萄的雄蕊接种在PIV培养基上胚性愈伤组织诱导率最高(10.8%);初生原胚团细胞核大、染色深且具有旺盛分裂能力,二次再生途径诱导的次生原胚团具有同样的细胞学特征且可以正常成苗。【结论】葡萄体细胞胚诱导受基因型、外植体类型影响显著;二次胚再生途径可以长期诱导、保存和扩繁原胚团,能为葡萄遗传转化持续提供受体材料。

关键词(KeyWords)： 葡萄;体细胞胚;二次胚再生;次生原胚团;细胞学观察

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31372022);; 陕西省科技研究发展(攻关)计划(2014K02-02-03)

作者(Author): 程远;万东艳;张宝林;王跃进;文颖强;

Email:

DOI: 10.13925/j.cnki.gsxb.20170011

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