师校欣;杜国强;马宝焜;王惠英;李玎;

• 1:河北农业大学园艺学院
• 2:河北农业大学园艺学院
• 3:河北农业大学园艺学院
• 4:河北农业大学园艺学院

摘要(Abstract)：

叶片再生效率的高低直接影响目的基因转化的成功率,为建立稳定、高效的樱桃不定芽离体再生体系,以30～40d苗龄的甜樱桃砧木ZY-1的组培继代苗为试材,取上部幼嫩叶片或不含腋芽的茎段,分别从培养基生长调节剂配比、接种材料类型、叶片接种部位、接种方式以及培养条件等方面进行了不定芽再生技术研究。结果表明,培养基中添加7.0mg/L的6-BA与0.5～1.0mg/L的IBA配比时不定芽再生率和出芽数均较高,TDZ和NAA不适于诱导ZY-1叶片再生不定芽;接种继代苗茎段比叶片再生率高;接种叶片以选择嫩叶横切2刀、远轴面向下接触培养基的方式为好;叶片不同部位处理以带叶柄的基部叶块最易再生;叶片接种后在25℃室温条件下,先暗培养3周再照光,利于不定芽的再生。

关键词(KeyWords)： 樱桃砧木;ZY-1;离体叶片;茎段;不定芽

Abstract:

Keywords:

基金项目(Foundation): 河北省自然科学基金资助项目(C2005000192);; 河北农业大学博士、引进人才启动基金资助项目

作者(Author): 师校欣;杜国强;马宝焜;王惠英;李玎;

Email:

DOI:

参考文献(References)：

• [1]HUANGZG,ZHAOGR,HANLX,et al(黄贞光,赵改荣,韩礼星,等).The development tendency of rootstocks of sweet cherry in overseas[J].Fruit Grower’s Friend(果农之友),2004,1:43-44.(in Chinese)
• [2]WANGGL,XIAXY,ZHONGWT,et al(王关林,夏秀英,钟文田,等).Regeneration and antibacterial peptide gene transformation of cherryleaves[J].Acta Horticulturae Sinica(园艺学报),2003,30(2):209-211.(in Chinese)
• [3]HUANG W J,LIU Q Z,ZHAO H J,et al(黄文江,刘庆忠,赵红军,等).Regeneration ofshoots fromin vitroleaves ofMahaleb cherry[J].Deciduous Fruits(落叶果树),2002(4):1-3.(in Chinese)
• [4]LIUQZ,ZHAOH J,LI ZQ(刘庆忠,赵红军,李志强).Plant re-generation from in vitro leaves of cherry dwarf rootstocks[J].Journal ofFruit Science(果树学报),2001,18(15):255-257.(in Chinese)
• [5]SUN Q R,L B,ZHANG L S,et al(孙清荣,李勃,张力思,等).Shoot regeneration fromleaves of sweet cherry(Prunus avium L.)in vitro[J].Deciduous Fruits(落叶果树),2001(1):10-11.(in Chinese)
• [6]LIUR D,LIAOMA(刘仁道,廖明安).Researches of micropropa-gation for ZY-1dwarfrootstocks ofsweet cherry[J].Northern Horti-culture(北方园艺),2004(5):61-63.(in Chinese)
• [7]SHI X X,DU G Q,GAO Y,et al(师校欣,杜国强,高仪,等).High efficient adventitious shoot regeneration from leaves in vitro of Apple cultivars[J].Journal ofFruit Science(果树科学),1999,16(4):255-258.(in Chinese)
• [8]MICHALCZUK B,MICHALCZUK L.The effect of light quality on regeneration rate and plantlet development in transgenic Petunia Revolution(Surfinia type)[J].Acta Horticulturae,2000(530):397-401.
• [9]SEONJ,CUI YY,KOZAI T,et al.Influence ofin vitrogrowth con-ditions on photosynthetic competence and survival rate of Rehman-nia glutinosa plantlets during acclimatization period[J].Plant Cell,Tissue and Organ Culture,2000,61(2):135-142.
• [10]KORBANSS,O’CONNOR P A,ELOBEIDYA.Effect ofthidazuron,naphthaleneacetic,and dark incubation and genotype on shoot organogenesis fromMalus leaves[J].Journal ofHorticultural Science,1992,67(30):341-349.
• [11]SHI X X,DU G Q,MA J L,et al(师校欣,杜国强,马俊莲,等).Callus formation and adventitious bud regeneration from leaves in vitro in Persimmon(Diospyros kaki Thunb.)[J].Journal of Fruit Sci-ence(果树学报),2004,21(4):376-378.(in Chinese)