焦竹青;许培磊;王振兴;艾军;刘迎雪;秦红艳;杨义明;

• 1:中国农业科学院特产研究所

摘要(Abstract)：

【目的】为开展山葡萄花序蛋白质组学研究。【方法】以山葡萄(Vitis amurensis Rupr)花序为试材,通过对蛋白质提取方法、裂解液成分、胶条pH梯度的改进,建立了适于山葡萄花序蛋白质组学研究的最佳双向电泳技术体系。【结果】采用酚提取法提取花序蛋白质,用含有硫脲和DTT的裂解液Ⅱ裂解,选用17 cm pH 4～7的IPG胶条在适宜的双向电泳条件下分离,可以获得分辨率高、背景清晰且重复性好的双向电泳图谱,可以检测出1 088个清晰的蛋白点。【结论】酚提取法适于山葡萄花序蛋白质的提取,硫脲和DTT可增强花序蛋白质的溶解性。

关键词(KeyWords)： 山葡萄花序;蛋白质提取;双向电泳技术

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系建设专项(nycytx-30-01);; 山葡萄种质资源收集、编目与利用项目(NB2011-2130135-37);; 国家山葡萄种质资源子平台运行服务项目(科技部)

作者(Authors): 焦竹青;许培磊;王振兴;艾军;刘迎雪;秦红艳;杨义明;

DOI: 10.13925/j.cnki.gsxb.2012.05.039

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