杨加付;赵宣;刘菊;杨晓东;王士伟;杨景华;张明方;

• 1:温州科技职业学院
• 2:浙江大学园艺系

摘要(Abstract)：

枯萎病是甜瓜等瓜类作物的毁灭性病害,Fom-2基因介导了甜瓜抗枯萎病生理小种0和1,抗枯萎病育种是甜瓜等瓜类作物育种的重要目标之一。通过同源克隆方法从薄皮甜瓜白啄瓜中克隆到了枯萎病抗性基因Fom-2,推定编码1 099个氨基酸,所推定的蛋白质序列经Blast分析与数据库中NBS-LRR类R基因具有较高的同源性。进一步序列分析FOM-2蛋白具有典型的R基因的NB-ARC及SCOP 2个结构功能域。实时荧光定量PCR结果表明,白啄瓜Fom-2基因经枯萎病接种后表达明显上调。白啄瓜Fom-2基因为白啄瓜抗病育种奠定了基础。

关键词(KeyWords)： 薄皮甜瓜;白啄瓜;枯萎病;Fom-2基因

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金项目(Y3080084);; 浙江省科技计划项目(2008C22060)

作者(Author): 杨加付;赵宣;刘菊;杨晓东;王士伟;杨景华;张明方;

Email:

DOI: 10.13925/j.cnki.gsxb.2011.06.030

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