王美军;黄乐;蒋建雄;杨国顺;刘昆玉;钟晓红;石雪晖;

• 1:湖南农业大学园艺园林学院
• 2:湖南省葡萄工程技术研究中心
• 3:湖南农业大学生物科学技术学院

摘要(Abstract)：

【目的】探讨野生刺葡萄(Vitis davidii Foёx)的遗传多样性、各类型之间的亲缘关系及演变关系。【方法】利用扫描电镜对25份野生刺葡萄的花粉形态特征进行系统观察和分析。【结果】除供试材料澧县雌能花花粉形状不规则外,其他材料花粉粒为超长圆球形和长圆球形;两性花与雄能花具3条萌发沟;花粉粒极轴长变化为23.05~31.95μm,变异系数7.71%,赤道轴长变化为12.85~20.60μm,变异系数10.36%;花粉外壁极面纹饰呈穴状、网状、疣状及2者间的过渡状态。【结论】基于花粉粒由大到小的演化顺序,将刺葡萄各类型分为3类;刺葡萄花粉粒极面纹饰的演化趋势为:穴状-网状-疣状。刺葡萄花粉极面外壁纹饰的差异与花粉粒大小可作为研究其遗传多样性、亲缘性关系与演变进程的依据。

关键词(KeyWords)： 刺葡萄;花粉形态;分类

Abstract:

Keywords:

基金项目(Foundation): 国家农业产业技术体系专项资金(CARS-30);; 国家农业部农业公益性行业科研项目(nyhyzx07-027);; 湖南省教育厅项目(07C010);; 湖南省研究生科研创新项目(CX2012B300)

作者(Author): 王美军;黄乐;蒋建雄;杨国顺;刘昆玉;钟晓红;石雪晖;

Email:

DOI: 10.13925/j.cnki.gsxb.20130484

参考文献(References)：

• [1]SHI Xue-hui,YANG Guo-shun,XIONG Xing-yao,LIU Kun-yu,ZHONG Xiao-hong,WANG Xian-rong,NI Jian-jun,GUO Guangyin.Research and utilization status Quo of germplasm resources of Vitis davidii Foёx in hunan[J].Hunan Agricultural Sciences,2010(19):1-4.石雪晖,杨国顺,熊兴耀,刘昆玉,钟晓红,王先荣,倪建军,郭光银.湖南省刺葡萄种质资源的研究与利用[J].湖南农业科学,2010(19):1-4.
• [2]JIN Yan,SHI Xue-hui,XIONG Xing-yao,QIN Dan.Research and utilization status Quo of germplasm resources of Vitis davidii Foёx[J].Sine-overseas Grapevine and Wine,2008(4):60-62,69.金燕,石雪晖,熊兴耀,秦丹.刺葡萄种质资源的研究与利用现状[J].中外葡萄与葡萄酒,2008(4):60-62,69.
• [3]SHI Xue-hui,WANG Yi-zhi,CHEN Zu-yu,YANG Guo-shun,LIU Kun-yu,ZHONG Xiao-hong,CHEN Jian-ye,LONG Gui-you.The preliminary study on the botanical character and the disease resistance of hunan brier grape[J].Sine-overseas Grapevine and Wine,2002(2):22-24.石雪晖,王益志,陈祖玉,杨国顺,刘昆玉,钟晓红,陈建业,龙桂友.湖南刺葡萄植物学性状及抗病性研究初报[J].中外葡萄与葡萄酒,2002(2):22-24.
• [4]WANG Yue-jin,WANG Xi-ping,ZHOU Peng,ZHENG Xue-qin.A RAPD marker linked to anthracnose resistant gene of wild grapes native to China[J].Acta Horticulture Sinica,2000,27(5):321-325.王跃进,王西平,周鹏,郑学勤.中国野生葡萄抗黑痘病基因的RAPD标记[J].园艺学报,2000,27(5):321-325.
• [5]WANG Yue-jin,HE Pu-chao.Study on the inheritance of resistance to powdery mildew in Chinese native wild Vitis L.Species[J].Scientia Agricultura Sinica,1997,30(1):20-26.王跃进,贺普超.中国葡萄属野生种叶片抗白粉病遗传研究[J].中国农业科学,1997,30(1):20-26.
• [6]ZHANG Ying,SUN Hai-sheng,FAN Xiu-cai,JIANG Jian-fu,LIU Chong-huai.Identification and evaluation of resistance of Vitis to grape white rot[J].Journal of Fruit Science,2013,30(2):191-196.张颖,孙海生,樊秀彩,姜建福,刘崇怀.中国野生葡萄资源抗白腐病鉴定及抗性种质筛选[J].果树学报,2013,30(2):191-196.
• [7]DUAN Hui.The preliminary study on resistance mechanism of Vitis davidii Foёx to downy mildew and botrytis cinerea[D].Changsha:Hunan Agricultural University,2013.段慧.刺葡萄对霜霉病与灰霉病的抗性机理初探[D].长沙:湖南农业大学,2013.
• [8]WANG Yue-jin,XU Yan,ZHANG Jian-xia,ZHOU Peng.Identification of RAPD markers linked to ripe rot resistant gene in wild grapes native to China[J].Scientia Agricultura Sinica,2002,35(5):536-540.王跃进,徐炎,张剑侠,周鹏.中国野生葡萄果实抗炭疽病基因的RAPD标记[J].中国农业科学,2002,35(5):536-540.
• [9]LIU Kun-yu,FANG Fang,SHI Xue-hui,YANG Guo-shun,ZHONG Xiao-hong,NI Jian-jun,XU Feng,BAI Miao,NIE Song-qing,CHEN Xiang-yun.Resistance of Vitis adenoclada Hand.-Mazz.and Vitis davidii Foёx to sphaceloma ampelinum and plasmopara viticola[J].Journal of Hunan Agricultural University:Natural Sciences Edition,2013,39(1):46-51.刘昆玉,方芳,石雪晖,杨国顺,钟晓红,倪建军,徐丰,白描,聂松青,陈湘云.腺枝葡萄与刺葡萄对葡萄黑痘病和霜霉病的抗性[J].湖南农业大学学报:自然科学版,2013,39(1):46-51.
• [10]QIN Dan,XIONG Xing-yao,SHI Xue-hui,HU Ya-ping.The study on the quality and the processing characteristic of brier grape[J].Academic Periodical of Farm Produce Processing,2006(1):8-10.秦丹,熊兴耀,石雪晖,胡亚平.刺葡萄的品质分析及加工性能研究[J].农产品加工·学刊,2006(1):8-10.
• [11]XU Feng,SHI Xue-hui,YANG Guo-shun,LIU Kun-yu,NI Jianjun,XIONG Xing-yao,LUO Bin-bin.Botanical characteristics of various Vitis davidii Foёx in hunan province[J].Sine-overseas Grapevine and Wine,2010(3):8-12.徐丰,石雪晖,杨国顺,刘昆玉,倪建军,熊兴耀,罗彬彬.湖南不同类型刺葡萄植物学性状研究[J].中外葡萄与葡萄酒,2010(3):8-12.
• [12]HUANG Le,WANG Mei-jun,JIANG Jian-xiong,YANG Guo-shun,LIU Kun-yu,ZHONG Xiao-hong,WANG Xian-rong,SHI Xue-hui.Morphological characteristics of floral organs of Vitis davidii Foёx.[J].Hunan Agricultural Sciences,2013(15):31-33.黄乐,王美军,蒋建雄,杨国顺,刘昆玉,钟晓红,王先荣,石雪晖.刺葡萄花器官形态特征研究[J].湖南农业科学,2013(15):31-33.
• [13]ZHANG Meng,LIAN Chun-lan,MATSUKI Yu,WANG San-hong,TAO Jian-min.Genetic diversity analysis of wild Vitis davidii in huangshan region,Anhui province based on SSR molecular markers[J].Acta Agriculture Jiangxi,2012,24(6):9-12.张萌,练春兰,松木悠,王三红,陶建敏.基于SSR分子标记的安徽黄山地区野生刺葡萄的遗传多样性分析[J].江西农业学报,2012,24(6):9-12.
• [14]LIU Kun-yu,XU Feng,SHI Xue-hui,ZHONG Xiao-hong,YANG Guo-shun,NI Jian-jun.Relationship analysis for Vitis davidii Foёx with SRAP molecular markers[J].Journal of Hunan Agricultural University:Natural Sciences Edition,2012,38(6):607-611.刘昆玉,徐丰,石雪晖,钟晓红,杨国顺,倪建军.基于SRAP标记的刺葡萄亲缘关系分析[J].湖南农业大学学报:自然科学版,2012,38(6):607-611.
• [15]HE Pu-chao,CHAO Wu-ji.Scanning electron microscopy study on the pollen of Chinese wild Vitis species[J].China Fruits,1982(3):43-45.贺普超,晁无疾.我国葡萄属野生种花粉电子显微镜扫描研究[J].中国果树,1982(3):43-45.
• [16]NI Sui,LI JI-yuan,TIAN Min,GAO Han-dong.Pollen exine sculpture of Sect.camellia in genuscamellia and its taxonomic significance[J].Journal of Nanjing Forestry University:Nnatural Science Edition,2007,31(4):16-20.倪穗,李纪元,田敏,高捍东.红山茶组植物花粉外壁纹饰特征及其演化关系[J].南京林业大学学报:自然科学版,2007,31(4):16-20.
• [17]YANG Xiang-hui,WU Ying-xin,LIN Shun-quan.SEM observation on the pollen morphology of six eriobotrya plants[J].Journal of Fruit Science,2009,26(4):572-576.杨向晖,吴颖欣,林顺权.6种枇杷属植物花粉形态扫描电镜观察[J].果树学报,2009,26(4):572-576.
• [18]NIU Li-xin,ZHANG YAN-long.The study on pollen morphology of the wild vitis varieties in China[J].Acta Horticulture Sinica,2000,27(5):361-363.牛立新,张延龙.中国野生葡萄花粉形态学研究[J].园艺学报,2000,27(5):361-363.
• [19]ERDTMAN G.Pollen morphology and plant taxonomy[M].Beijing:Science Press,1978:13-26.G.埃尔特曼.孢粉学手册[M].北京:科学出版社,1978:13-26.
• [20]WANG Xiao-dong,CHEN Xin-qi,LI Chao-luan.A study on pollen morphology of the genus Vitis L.[J].Acta Phytotaxonomica Sinica,2000,38(1):43-52.王晓东,陈心启,李朝銮.葡萄属植物花粉形态的研究[J].植物分类学报,2000,38(1):43-52.
• [21]WANG Kai-fa.Palynology introduction[M].Beijing:Peking University Press,1983:21-25.王开发.孢粉学概论[M].北京:北京大学出版社,1983:21-25.
• [22]KONG Qing-shan.Chinese grape[M].Beijing:China Agricultural Science and Technology Press,2004:16-27.孔庆山.中国葡萄志[M].北京:中国农业科技出版社,2004:16-27.
• [23]LIU Chong-huai,FENG Jian-can,JIANG Jian-fu.Cluster analysis of Chinese wild grape species based on morphological characters[J].Journal of Plant Genetic Resources,2011,12(6):847-852.刘崇怀,冯建灿,姜建福.中国葡萄属植物形态学聚类分组研究[J].植物遗传资源学报,2011,12(6):847-852.
• [24]EDLUND A F,SWANSON R,PREUSS D.Pollen and stigma structure and function:the role of diversity in pollination[J].The Plant cell,2004,(16):84-97.
• [25]JAN MULLER.Form and function in angiosperm pollen[J].Annals of the Missouri Botanical Garden,1979,66(4):596-632.
• [26]LI Run-tang,WEI Wen-na.Study on botanic characteristic and pollen morphology of wild grapes in hunan[J].Journal of Hunan Agricultural University,2000,26(2):102-104.李润唐,魏文娜.湖南三种野生葡萄的生物学特性及花粉形态研究[J].湖南农业大学学报,2000,26(2):102-104.
• [27]CHAO Wu-ji,YUAN Zhi-fa.Discussion on classification and relationship of Chinese wild species of grape[J].Acta university agricultural Boreali-occidentalis:Natural Sciences Edition,1990,18(2):7-12.晁无疾,袁志发.我国葡萄属植物分类与亲缘关系的探讨[J].西北农林科技大学学报:自然科学版,1990,18(2):7-12.
• [28]LIU San-jun,KONG Qing-shan.Studies on the taxonomy wild grape species native to China[J].Journal of Fruit Science,1995,12(4):224-227.刘三军,孔庆山.我国野生葡萄分类研究[J].果树科学,1995,12(4):224-227.
• [29]WEI Zhong-xin.Pollen ultrastructure of Theaceae and its systematic significance[J].Acta Botanica Yunnanica,1997,19(2):143-153.韦仲新.山茶科花粉超微结构及其系统学意义[J].云南植物研究,1997,19(2):143-153.
• [30]GUO Guo-nan,WANG Li-rong,YAN Zhen-li,Zhu Geng-rui,FANG Wei-chao.Study on the variation and evolution of peach its related wild species by pollen morphology[J].Journal of Fruit Science,2006,23(5):664-669.过国南,王力荣,阎振立,朱更瑞,方伟超.利用花粉粒形态分析法研究桃种质资源的进化关系[J].果树学报,2006,23(5):664-669.
• [31]WALKER J W.Evolutionary of exine structure in the pollen of the primitive angiosperms[J].Botanical Society of America,1974,61(8):891-902.
• [32]LIU Hong-chang,HU Yue-wei,CHAI Kun,WANG Hua-lei,ZHAO Zhi.Pollen morphology and evolution trend in polygonum multiflorum thunb.Germplasms[J].Acta Botanica Boreali-Occidentalia Sinica,2013,33(7):1353-1367.刘红昌,胡跃维,柴琨,王华磊,赵致.何首乌不同种质花粉形态及进化趋势研究[J].西北植物学报,2013,33(7):1353-1367.
• [33]HE Yong-hua,LI Chao-luan,CAO Ya-ling.Comparative anatomy of vegetative organs in the genus Vitis L.and its systematic significance[J].Acta Phytotaxonomica Sinica,1994,32(2):154-164.何永华,李朝銮,曹亚玲.葡萄属营养器官的比较解剖学及其系统学意义[J].植物分类学报,1994,32(2):154-164.