严红,燕继晔,王忠跃,李亚宁,金桂华,李兴红

• 1:北京市农林科学院植物保护环境保护研究所
• 2:中国农业科学院植物保护研究所
• 3:河北农业大学植物保护学院

摘要(Abstract)：

【目的】为检测葡萄灰霉病菌对多菌灵、腐霉利和乙霉威3种杀菌剂的多重抗药性,指导生产上葡萄灰霉病的药剂防治。【方法】从甘肃、山东、北京等14个省、市、自治区采集葡萄灰霉病样分离纯化得到109个灰葡萄孢(Botrytis cinerea)单孢系菌株,采用最低抑制浓度法(MIC)测定了葡萄灰霉病菌对苯并咪唑类(多菌灵)、二甲酰亚胺类(腐霉利)和氨基甲酸酯类(乙霉威)杀菌剂的多重抗药性。【结果】结果表明,葡萄灰霉病菌对多菌灵、腐霉利和乙霉威的抗药性菌株率分别达到了83.5%、14.7%和17.4%;抗药性类型有BenRDicSNPCS、BenRDicRNPCS、BenRDicRNPCR和BenSDicSNPCR4种,所占比例分别为68.8%、13.8%、0.9%和16.5%。【结论】我国不同地区的葡萄灰霉病菌对苯并咪唑类、二甲酰亚胺类和氨基甲酸酯类杀菌剂普遍存在抗药性问题,对多菌灵的抗药性较为严重,因此,在生产上,应选择一些替代的新型杀菌剂和生物农药。

关键词(KeyWords)： 葡萄;灰霉病菌;多菌灵;腐霉利;乙霉威;抗药性

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系专项资金资助(CARS-30)

作者(Author): 严红,燕继晔,王忠跃,李亚宁,金桂华,李兴红

DOI: 10.13925/j.cnki.gsxb.2012.04.002

参考文献(References)：

• [1]BOUCHRA C,ACHOURI M,IDRISSI HASSANI L M,HMAMOUCHIM.Chemical composition and antifungal activity of essential oils ofseven Moroccan Labiates against Botrytis cinerea Pers:Fr.[J].Journalof Ethnopharmacology,2003,89:165-169.
• [2]ELAD Y,YUNIS H,KATAN T.Multiple fungicide resistance tobenzimidazole,dicarboximides and diethofencarb in field isolates ofBotrytis cinerea in Israel[J].Plant Pathology,1992,41:41-46.
• [3]NORTHOVER J,MATTEON J A.Resistance of Botrytis cinerea tobenomyl and iprodione in vineyards and greenhouses after exposureto the fungicides alone or mixed with captan[J].Plant Disease,1986,70(5):398-402.
• [4]LATORRE B,FLORES V,SARA A,ROCO A.Dicarboximide-re-sistant strains of B.cinerea from table grapes in Chile:Survey andcharacterization[J].Plant Dis,1994,78:990-994.
• [5]ESTERIO M,MUNOZ G,RAMOS C,COFRE G,ESTEVEZ R,SALINAS A,AUGER J.Characterization of Botrytis cinerea isolatespresent in Thompson seedless table grapes in the Central Valley ofChile[J].Plant Dis,2011,95:683-690.
• [6]ZHOU Ming-guo,YE Zhong-yin,LIU Jing-fen.Detection of resis-tance of grey mould(Botrytis cinerea)to benzimidazoles in the field[J].Journal of Nanjing Agricultural University,1987,10(2):53-57.周明国,叶钟音,刘经芬.南京市郊灰霉菌对苯并咪唑类杀菌剂田间抗性的检测[J].南京农业大学学报,1987,10(2):53-57.
• [7]LIU Bo,LIU Jing-fen,YE Zhong-yin,ZHOU Ming-guo.On theresistant strains induced by procymidone[J].Acta PhytopathologicaSinica,1993,23(1):79-80.刘波,刘经芬,叶钟音,周明国.药剂诱导灰葡萄孢产生速克灵抗性菌株的研究[J].植物病理学报,1993,23(1):79-80.
• [8]KATAN T,EALD Y,YUNIS H.Resistance to diethofancatb(NPC)in benomyl-resistant field isolates of Botrytis cinerea[J].Plant Pathol-ogy,1989,38:86-92.
• [9]FARETRA F,POLLASTRO S.Genetics of sexual compatibility andresistance to benzimidazole and dicarboximide fungicides in isolatesof Botryotinia fuckeliana(Botrytis cinerea)from some countries[J].Plant Pathology,1993,42(1):42-48.
• [10]JI Ming-shan,CHENG Gen-wu,ZHANG Yi-xian,BAI Jian-yu,HUANG Li-hui.Studies on resistance to carbendazim and di-ethofencarb of Botrytis cinerea[J].Journal of Shenyang AgriculturalUniversity,1998,29(3):213-216.纪明山,程根武,张益先,白剑宇,黄丽辉.灰霉病菌对多菌灵和乙霉威抗性研究[J].沈阳农业大学学报,1998,29(3):213-216.
• [11]ZHANG Cong-yu,ZHANG Zi-xue,CUI Guang-rong.Detection andmanagement of the resistance of Botrytis cinerea to fungicides in An-hui Province[J].Plant Protection,2006,32(3):32-34.张从宇,张子学,崔广荣.安徽省番茄灰霉菌抗药性测定和治理[J].植物保护,2006,32(3):32-34.
• [12]LEROUX P,CHAPELAND F,DESBROSSES D,GREDT M.Pat-terns of cross-resistance to fungicides in Botryotinia fuckeliana(Botrytis cinerea)isolates from French vineyards[J].Crop Protec-tion,1998,18:687-697.
• [13]CHEN Yu-fei,WEN Jing-zhi,LI Li-jun.Research advance ofgrape grey mould[J].Journal of Northeast Agricultural University,2006,37(5):693-699.陈宇飞,文景芝,李立军.葡萄灰霉病研究进展[J].东北农业大学学报,2006,37(5):693-699.
• [14]QIAO Guang-hang,YAN Hong,YAO Yi-qing,HUANG Jin-bao,LI Xing-hong.Detection of multiple fungicide resistance in Botrytiscinerea from tomato in Beijing[J].Plant Protection,2011,37(5):176-180.乔广行,严红,么奕清,黄金宝,李兴红.北京地区番茄灰霉病菌的多重抗药性检测[J].植物保护,2011,37(5):176-180.
• [15]XU Zuo-ting,LI Lin,YU Jian-lei,ZHAO De-you,QI Jun-shan.Study on Resistance variation of Botrytis cinerea to procymidone[J].Shandong Agricultural Sciences,1999(2):30-38.徐作珽,李林,于建垒,赵德友,齐军山.蔬菜灰霉病菌对速克灵抗药性变异研究[J].山东农业科学,1999(2):30-38.
• [16]DING Zhong,LIU Feng,MU Li-yi.Study on susceptibility of differ-ent resistance type of Botrytis cinerea to Fungicides with differentmechanism[J].Chinese Journal of Pesticide Science,2001,3(4):59-63.丁中,刘峰,慕立义.不同抗性型灰葡萄孢菌Botrytis cinerea对不同作用机制杀菌剂的敏感性研究[J].农药学学报,2001,3(4):59-63.
• [17]YARDEN O,KATAN T.Mutations leading to substitutions at aminoacids 198 and 200 of Beta-tubulin that correlated with benomyl-re-sistance phenotypes of field strains of Botrytis cinerea[J].Phytopathol-ogy,1993,83(12):1478-1483.
• [18]DING Zhong,LIU Feng,WANG Hui-li,WU Chun-xian,MU Li-yi.Studies on multiple fungicide resistance to Botrytis cinerea[J].Journal of Shandong Agricultural University:Natural Science Edi-tion,2001,32(4):452-456.丁中,刘峰,王会利,吴春先,慕立义.番茄灰霉菌的多重抗药性研究[J].山东农业大学学报:自然科学版,2001,32(4):452-456.
• [19]RAPOSO R,DELCAN J,GOMEZ V,MELGAREJO P.Distributionand fitness of isolates of Botrytis cinerea with multiple fungicide re-sistance in Spanish greenhouses[J].Plant Pathology,1996,45(3):497-505.
• [20]KIM B S,PARK E W,CHO K Y.Population dynamics of sensitiveand resistance phenotypes of Botrytis einerea to benzimidazole,di-carboximide and N-phenylearbamate fungicides in Korea[J].Journalof Pesticide Science,2000,25(4):385-386.
• [21]ZHANG Tao-tao,LI Xing-hong,YAN Hong,LI Ming-yuan.De-tection of the resistance of Botrytis cinerea to fungicides in Beijingsuburbs[C]//Crops Pest Control Research(Volume One).Beijing:China Agricuture Press,2001:203-205.张涛涛,李兴红,严红,李明远.京郊蔬菜灰霉病菌抗药性测定[C]//经济作物有害生物防治研究进展(上册).北京:中国农业出版社,2001:203-205.
• [22]ZHU Gui-ning,HUANG Fu-xin,CAI Jian-he,QIN Bi-xia,LIUZhi-ming.Multiple fungicide-resistance of tomato Botrytis cinereain Guangxi[J].China Vegetables,2003(4):14-16.朱桂宁,黄福新,蔡健和,秦碧霞,刘志明.广西番茄灰霉病菌的多重抗药性检测[J].中国蔬菜,2003(4):14-16.