龙治坚;王莹;向素琼;梁国鲁;

• 1:西南大学园艺园林学院

摘要(Abstract)：

经过10多年的发展,DNA分子标记已经在枇杷属植物中得到较为广泛的应用。概述了枇杷属植物研究中使用的DNA分子标记类型及特点,并从种质资源遗传多样性与亲缘关系分析、分子遗传连锁图谱构建、种质鉴定与指纹图谱构建等方面总结了DNA分子标记技术在枇杷上的研究进展。同时,对DNA分子标记技术在枇杷研究中存在的问题进行讨论。此外,认为枇杷更多分子标记类型的开发和应用、种质资源的收集与深度分子评价、高密度分子遗传图谱和种质指纹图谱的构建是今后的发展方向。

关键词(KeyWords)： 枇杷;分子标记;遗传多样性;分子辅助育种

Abstract:

Keywords:

基金项目(Foundation): 重庆市自然科学基金(CSTC,2010BB1132);; 国家农业部公益性行业(农业)科研专项(201003073);; 教育部博士点基金(20110182110013)

作者(Author): 龙治坚;王莹;向素琼;梁国鲁;

Email:

参考文献(References)：

• [1]YANG Xiang-hui.Systematically stud ying for Eriobotrya[D].Guangzhou:South China Agricultural University,2005.杨向晖.枇杷属植物的系统研究[D].广州:华南农业大学,2005.
• [2]BADENES M L,LIN Shun-quan,YANG Xiang-hui,LIU Cheng-ming,HUANG Xu-ming.Loquat[M]/In:K.M.Folta,S.E.Gardiner(es).Genetics and Genomics of Rosaceae,Plant Genetics and Ge-nomics:Crops and Models.Springer Sciecce+Springer Media LLC,2009,525-538.
• [3]LIN Shun-quan,YANG Xiang-hui,LIU Cheng-ming,HU You-li,HE Ye-hua,HU Gui-bing,ZHANG Hai-lan,HE Xiao-long,LIUYue-xue,LIU Zong-li.Natural geographical distribution of genusEriobotrya plants in China[J].Acta Horticulturae Sinica,2004,31(5):569-573.林顺权,杨向晖,刘成明,胡又厘,何业华,胡桂兵,张海岚,何小龙,刘月学,刘宗莉.中国枇杷属植物的自然地理分布[J].园艺学报,2004,31(5):569-573.
• [4]AGARWAL M,SHRIVASTAVA N,PADH H.Advances in molecu-lar marker techniques and their applications in plant sciences[J].Plant Cell Rep,2008,27:617-631.
• [5]GUARINO C,SANTORO S,D E SIMONE L,LAIN O,CIPRIANIG,TESTOLIN R.Genetic diversity in a collection of ancient culti-vars of apple(Malus×domestica Borkh.)as revealed by SSR-basedfingerprinting[J].Journal of Horticultural Science&Biotechnology,2006,8(1):39-44.
• [6]DONDINI L,PIERANTONI L,GAIOTTI F,CHIODINI R,TAR-TARINI S,BAZZI C,SANSAVINI S.Identifying QTLs for fire-blight resistance via a European pear(Pyrus communis L.)geneticlinkage map[J].Molecular Breeding,2004,14:407-418.
• [7]CHENG Zhong-ping,CHEN Zhi-wei,HU Chun-gen,DENG Xiu-xin,LUO Zheng-rong.The identification and systematic relation-ship analysis of Amygdalus spp.by molecular markers[J].Journal ofHuazhong Agricultural University,2001,20(3):199-204.程中平,陈志伟,胡春根,邓秀新,罗正荣.利用分子标记对桃属植物种的识别及其亲缘关系分析[J].华中农业大学学报,2001,20(3):199-204.
• [8]FOURNIER-LEVEL A,CUNFF L L,GOMEZ C,DOLIGEZ A,A-GEORGES A,ROUX C,BERTRAND Y,SOUQUET J M,CHEYNIER V,THIS P.Quantitative genetic bases of anthocyaninvariation in grape(Vitis vinifera L.ssp.sativa)berry:a quantitativetrait locus to quantitative trait nucleotide integrated study[J].Genet-ics Society of America,2009,183:1127-1139.
• [9]WANG Xiao-lan,CHIANG Tzen-yuh,ROUX N,HAO Gang,GEXue-Jun.Genetic diversity of wild banana(Musa balbisiana Colla)in China as revealed by AFLP markers[J].Genetic Resources andCrop Evolution,2007,54(5):1125-1132.
• [10]ANDERSEN J R,LUBBERSTEDT T.Functional markers in plants[J].Trends Plant Sci,2003,8(11):554-560.
• [11]YANG Jing-hua,WANG Shi-wei,LIU Xun-yan,YANG Jia-fu,ZHANG Ming-fang.Development and application of functionalmarkers in higher plants[J].Scientia Agricultura Sinica,2008,41(11):3429-3436.杨景华,王士伟,刘训言,杨加付,张明方.高等植物功能性分子标记的开发与利用[J].中国农业科学,2008,41(11):3429-3436.
• [12]VILANOVA S,BADENES M L,MARINEZ-CALVO J,LLACER G.Analysis of loquat(Eriobotrya japonica Lindl)germplasm by RAPDmolecular markers[J].Euphytica,2001,121:25-29.
• [13]YANG Xiang-hui,LI Ping,LIU Cheng-ming,LIN Shun-quan.Genetic diversity in Eriobotrya genus and its closely related plantspecies using RAPD markers[J].Journal of Fruit Science,2009,26(1):55-59.杨向晖,李平,刘成明,林顺权.枇杷属植物及其近缘属植物亲缘关系的RAPD分析[J].果树学报,2009,26(1):55-59.
• [14]MENG Xiang-xun,PAN Xin-fa,CAO Guang-li,LI Meng-ying.Application of RAPD-PCR technique for diversity and identificationof loquat E.japonic Thunb Lindl varieties[J].Biotechnology Bulletin,2003(4):33-38.孟祥勋,潘新法,曹广力,李蒙英.枇杷栽培种的随机扩增DNA多态性(RAPD)研究[J].生物技术通报,2003(4):33-38.
• [15]CHEN Yi-ting,LAI Zhong-xiong,GUO Zhi-xiong,GUO Yu-qiong,CAI Ying-qing,CHEN Jing-ying,LU Xiu-min,WU Jin-shou.RAPD analysis of main species and cultivars in Eriobotrya[J].Acta Agriculturae Universitatis Jiangxiensis,2003,25(2):258-261.陈义挺,赖钟雄,郭志雄,郭玉琼,蔡英卿,陈菁瑛,陆修闽,吴金寿.枇杷主要种类的RAPD分析[J].江西农业大学学报,2003,25(2):258-261.
• [16]CHEN Yi-ting,LAI Zhong-xiong,CHEN Jing-ying,CAI Ying-qin,GUO Yu-qiong,WU Jin-shou.RAPD analysis of 65 acces-sions of Loquat Germplasm(Eriobotrya spp.)[J].Chinese Journal ofTropical Crops,2007,28(1):65-71.陈义挺,赖钟雄,陈菁瑛,蔡英卿,郭玉琼,吴金寿.65份枇杷种质资源的RAPD分析[J].热带作物学报,2007,28(1):65-71.
• [17]CHEN Yi-ting,LAI Zhong-xiong,CHEN Jing-ying,GUO Yu-qiong,CAI Ying-qing,GUO Zhi-xiong,WU Jin-shou,HUANGJin-song.RAPD analysis of genetic relationship among loquat culti-vars Zaozhong 6,Jiefangzhong and Moriowase[J].Journal of FujianAgriculture and Forestry University:Natural Science Edition,2004,33(1):46-50.陈义挺,赖钟雄,陈菁瑛,郭玉琼,蔡英卿,郭志雄,吴金寿,黄金松.枇杷品种‘早钟6号’与解放钟、森尾早生亲缘关系的RAPD分析[J].福建农林大学学报:自然科学版,2004,33(1):46-50.
• [18]CHEN Jing-ying,CHEN Yi-ting,LAI Zhong-xiong,CAI Ying-qing.RAPD analysis for Jiefangzhong loquat from 12 sites of FujianProvince[J].Subtropical Agriculture Research,2006,2(2):62-65.陈菁瑛,陈义挺,赖钟雄,蔡英卿.福建省12个地方解放钟枇杷的RAPD分析[J].亚热带农业研究,2006,2(2):62-65.
• [19]FAN Jian-xin,LUO Nan,WANG Yong-qing.RAPD Analysis on 8Cultivars or Lines of Loquat[Eriobotrya japonica(Thunb)Lindl][J].Journal of Sichuan Agricultural University,2006,24(1):65-68.范建新,罗楠,王永清.8个枇杷品种(系)的RAPD分析[J].四川农业大学学报,2006,24(1):65-68.
• [20]YANG Xiang-hui,LIU Cheng-ming,LIN Shun-quan.The geneticrelationship among Common loquat,Daduhe loquat and Oakleaf lo-quat,according to RAPD and AFLP analysis[J].Subtropical PlantScience,2007,36(2):9-12.杨向晖,刘成明,林顺权.普通枇杷、大渡河枇杷和栎叶枇杷遗传关系研究—基于RAPD和AFLP分析[J].亚热带植物科学,2007,36(2):9-12.
• [21]DONG Yan-ni.RAPD analysis of genetic diversity of seedlins fromminia ture seeds in loquat(Eriobotrya japonica Lindl)[D].Yaan:Sichuan Agricultural University,2008.董燕妮.枇杷小种子植株遗传多样性的RAPD分析[D].雅安:四川农业大学,2008.
• [22]FU Yan.RAPD and ISSR analysis of 41 accessions of loquat(Eri-obotrya Lindl.)[D].Yaan:Sichuan Agricultural University,2009.付燕.41份枇杷属植物材料的RAPD和ISSR分析[D].雅安:四川农业大学,2009.
• [23]QIAO Yan-chun,LIN Shun-quan,HE Xiao-long,YANG Xiang-hui.Identification of intraspecific and interspecific hybridizations inloquat(Eriobotrya)using RAPD molecular markers[J].Journal ofFruit Science,2010,27(3):385-390.乔燕春,林顺权,何小龙,杨向晖.普通枇杷种内和种间杂种苗的RAPD鉴定[J].果树学报,2010,27(3):385-390.
• [24]WANG Yong-qing,FU Yan,YANG Qin,LUO Nan,DENG Qun-xian,YAN Juan,ZENG Jian-guo,RUAN Guang-lun.Genetic di-versity of Eriobotrya analyzed by ISSR markers[J].Scientia SilvaeSinicae,2010,46(4):49-60.王永清,付燕,杨芩,罗楠,邓群仙,严娟,曾建国,阮光伦.枇杷属植物遗传多样性的ISSR分析[J].林业科学,2010,46(4):49-60.
• [25]SHENG Liang-ming,WANG Hua-kun,XU Chun-ming,ZHANGZhen.ISSR analysis of a new individual of white flesh loquat[J].Jiangsu Agricultural Sciences,2006,3:97-100.盛良明,王化坤,徐春明,章镇.白沙枇杷优良株系苏白1号的ISSR分析[J].江苏农业科学,2006,3:97-100.
• [26]FAN Chen-xin.Study on shoot regeneration in vitro and ISSR analy-sis of loquat[D].Nanjing:Nanjing Agricultural University,2008.范晨昕.白沙枇杷离体再生及ISSR分子标记研究[D].南京:南京农业大学,2008.
• [27]YANG Qin.Studies on mechanism of embryo abortion and geneticdiversity of seedlings from degenerated seeds by ISSR in loquat(Eri-obotrya japonica Lindl)[D].Yaan:Sichuan Agricultural University,2009.杨芩.枇杷胚败育机制及退化种子株系的ISSR遗传多样性研究[D].雅安:四川农业大学,2009.
• [28]QIAO Yan-chun,LIN Shun-quan,YANG Xiang-hui,LIU Yue-xue.Optimization of ISSR-PCR analysis and its application ingermplasm of loquat(E.japonica Lindl.cv)by uniform design[J].Genomics and Applied Biology,2009,28(1):123-126.乔燕春,林顺权,杨向晖,刘月学.均匀设计在枇杷ISSR-PCR反应体系优化中的应用[J].基因组学与应用生物学,2009,28(1):123-126.
• [29]FU Yan,LUO Nan,YANG Qin,WANG Yong-qing,DENG Qun-xian,LI Jun-qiang,QIN Hong-mei,RUAN Guang-lun.Estab-lishment and optimization of ISSR reaction system for species of Eri-obotrya[J].Journal of Fruit Science,2009,26(2):180-185.付燕,罗楠,杨芩,王永清,邓群仙,李俊强,秦红玫,阮光伦.枇杷属植物ISSR反应体系的建立和优化[J].果树学报,2009,26(2):180-185.
• [30]ZHAO Yi-jie,WANG Jiang-bo,ZHANG Xiao-hong,SHI Wei-shu,LIN Hang.Optimization of ISSR reaction system for species ofLoquat(Eriobotrya japonica Lindl.cv)[J].Chinese Agricultural Sci-ence Bulletin,2010,26(7):26-30.赵依杰,王江波,张小红,施维属,林航.枇杷ISSR扩增反应体系的优化[J].中国农学通报,2010,26(7):26-30.
• [31]ZHAO Yi-jie,WANG Jiang-bo,ZHANG Xiao-hong,SHI Wei-shu,LIN Hang.ISSR molecular identification of Eriobotrya japonica(Thunb.)Lindl.’Dong Huzao’[J].Chinese Journal of Tropical Crops,2010,31(1):72-78.赵依杰,王江波,张小红,施维属,林航.枇杷新品种‘东湖早’的ISSR分子鉴定[J].热带作物学报,2010,31(1):72-78.
• [32]XIE Li-xue,XU Jia-hui,ZHANG Li-jie,ZHANG Xiao-yan,ZHENG Shan,LI Tao.Genetic relationship analysis of 24 white-fleshed loquat(Eriobotrya japonica)[J].Fujian Journal of Agricultur-al Sciences,2012,27(3):261-266.谢丽雪,许家辉,张立杰,张小艳,郑姗,李韬.24份白肉枇杷种质资源的ISSR分析[J].福建农业学报,2012,27(3):261-266.
• [33]FAN Fu-hua,SONG Sha,HONG Yi,ZHAO Ya-nan,WEN Xiao-peng.Genetic diversity analysis of wild loquat(Eriobotrya japonica)germplasms from Guizhou province using ISSR markers[J].SouthChina Fruits,2012,41(3):36-39,54.范付华,宋莎,洪怡,赵亚楠,文晓鹏.贵州野生枇杷资源遗传多样性的ISSR分析[J].中国南方果树,2012,41(3):36-39,54.
• [34]FAN Fu-hua,YANG Yong-sheng,LI Qing-hong,DENG Yong,WEN Xiao-peng.Identification of new germplasms of Guizhou lo-quat using ISSR markers[J].Journal of Beijing Forestry University,2012,34(4):52-57.范付华,杨勇胜,李庆宏,邓勇,文晓鹏.利用ISSR分析技术鉴定贵州枇杷新种质的研究[J].北京林业大学学报,2012,34(4):52-57.
• [35]WU Jin-cheng,YANG Xiang-hui,LIN Shun-quan.Establishmentof AFLP analysis system and its application in loquat germplasm[J].Journal of Fruit Science,2006,23(5):774-778.吴锦程,杨向晖,林顺权.枇杷AFLP分析体系的建立与应用[J].果树学报,2006,23(5):774-778.
• [36]BIAN Yu.Analysis on genetic variation and DNA methylation of dif-ferent loquat Ploidies[D].Chongqing:Suothwest University,2010.边禹.不同倍性多倍体枇杷基因组变异及其DNA甲基化分析[D].重庆:西南大学,2010.
• [37]WANG Wei-xing.Genome variation and DNA methylation analysisof natural and artificial triploid loquats[D].Chongqing:SuothwestUniversity,2008.汪卫星.天然与人工合成三倍体枇杷基因组变异及其DNA甲基化分析[D].重庆:西南大学,2008.
• [38]GISBERT A D,MART魣NEZ-CALVO J,LL魱CER G,BADENES ML,ROMERO C.Development of two loquat(Eriobotrya japonica(Thunb.)Lindl.)linkage maps based on AFLP and SSR markersfrom different Rosaceae species[J].Molec,Breed,2009,23:523-538.
• [39]GISBERT A D,LOPEZ-CAPUZ I,SORIANO J M,LLACER G,ROMERO C,BADENES M L.Development of microsatellite mark-ers from loquat,Eriobotrya japonica(Thunb.)Lindl[J].PermanentGenetic Resources Note,2009,9(3):803-805.
• [40]GISBERT A D,ROMERO C,CALVO J M,LEIDA C,LL魣CER G,BADENES M L.Genetic diversity evaluation of a loquat(Eriobotryajaponica(Thunb)Lindl)germplasm collection by SSRs and S-allelefragments[J].Euphytica,2009,168:121-134.
• [41]WATANABE M,YAMAMOTOT,OHARA M,NISHITANIC,YA-HATA S.Cultivar differentiation identified by SSR markers and theapplication for polyploidy loquat plants[J].J Jpn Soc Hortic Sci,2008,77:388-394.
• [42]HE Qiao,LI Xiong-wei,LIANG Guo-lu,JI Kun,GUO Qi-gao,YUAN Wei-min,ZHOU Gen-zhi,CHEN Kun-song,W.ERICVAN DE WEG,GAO Zhong-shan.Genetic diversity and identity ofChinese loquat cultivars/accessions(Eriobotrya japonica)using ap-ple SSR markers[J].Plant Mol Biol Rep,2011,29:197-208.
• [43]HE Qiao.Genetic diversity analysis and cultivar indentification ofloquat(Eriobotlya japonica Lindl.)based on SSR[D].Chongqing:Suothwest University,2010.何桥.基于SSR标记的枇杷遗传多样性分析与品种鉴别[D].重庆:西南大学,2010.
• [44]HU Wen-shun,LI Tao,ZHENG Shan,JIANG Ji-mou,HUANGAi-ping,ZHANG Xi-juan,ZHENG Shao-quan,CHENG Liang.Genetic diversity and relationship analysis of 43 wild loquat(Eri-obotrya japonica)germplasm in Yunnan[J].Fujian Fruits,2010,4:20-28.胡文舜,李韬,郑姗,蒋际谋,黄爱萍,章希娟,郑少泉,陈亮.43份云南野生枇杷种质遗传多样性与亲缘关系分析[J].福建果树,2010,4:20-28.
• [45]ZHONG Yan.The nuclear DNA isolation from the leaves of loquat(Eriobotrya Lindl)plants and analysis of its SSR[D].Suzhou:Soo-chow University,2010.仲艳.枇杷核基因组DNA提取方法的改良及其SSR初步[D].苏州:苏州大学,2010.
• [46]SHENG Liang-ming,XUE Hua-bai,WANG Hua-kun,XU Chun-ming,WANG San-hong,ZHANG Zhen.Isolation of microsatellitemarkers in Eriobotrya japonica using 5’anchored PCR[J].JiangsuJournal of Agricultural Sciences,2007,23(1):50-53.盛良明,薛华柏,王化坤,徐春明,王三红,章镇.利用5’锚定PCR技术分离枇杷微卫星标记[J].江苏农业学报,2007,23(1):50-53.
• [47]XIANG Su-qiong,QUAN Zhi-wu,WANG Wei-xing,HE Bo,LIANG Guo-lu,DING Yi.Development and characterization of newpolymorphic microsatellite markers in loquat[C]//ISHS Acta Horti-culturae 887:III International Symposium on Loquat,2011:75-78.
• [48]YUE Na,HU Qian-qian,FANG Xing,QIU Xue-lin,CHU Chun-rong,QI Hong-li,MENG Xiang-xun.Fingerprinting patterns ofSSR markers constructed for 33 loquat[Eriobotrya japonica(Thunb.)Lindl.]germplasms[J].Chinese Agricultural Science Bulletin,2012,28(16):186-189.岳娜,胡倩倩,方星,邱学林,储春荣,祁红丽,孟祥勋.枇杷种质资源SSR标记指纹图谱的构建[J].中国农学通报,2012,28(16):186-189.
• [49]HAN Guo-hui,WANG Wei-xing,XIANG Su-qiong,BIAN Yu,GUO Qi-gao,HE Qiao,LI Xiao-lin,LIANG Guo-lu.Establish-ment and optimization of SCoT system in polyploidy loquats[J].Jour-nal of Fruit Science,2011,28(3):433-437.韩国辉,汪卫星,向素琼,边禹,郭启高,何桥,李晓林,梁国鲁.多倍体枇杷SCoT分析体系的建立与优化[J].果树学报,2011,28(3):433-437.
• [50]JIA Zhi-gang.Analysis on DNA methylation and SCoT markers ofprogeny from triploid loquat[D].Chongqing:Suothwest University,2011.贾志刚.三倍体枇杷实生后代及杂交后代SCoT标记及DNA甲基化分析[D].重庆:西南大学,2011.
• [51]QIAO Yan-chun,LIN Shun-quan,LIU Cheng-ming,YANG Xiang-hui.Optimization of SRAP analysis and its application in germplasmresearch of loquat(Eriobotrya japonica)[J].Journal of Fruit Science,2008,25(3):348-352.乔燕春,林顺权,刘成明,杨向晖.SRAP分析体系的优化及在枇杷种质资源研究上的应用[J].果树学报,2008,25(3):348-352.
• [52]UZUNA A,YESILOGLUB T,AKA-KACARB Y,TUZCUB O,GULSEN O.Genetic diversity and relationships within Citrus andrelated genera based on sequence related amplified polymorphismmarkers(SRAPs)[J].Scientia Horticulturae,2009,121(3):306-312.
• [53]MUKHLESUR R,PETER B E.MCVETTY,LI Gen-yi.Develop-ment of SRAP,SNP and Multiplexed SCAR molecular markers forthe major seed coat color gene in Brassica rapa L[J].Theoretical andApplied Genetics,2007,115(8):1101-1107.
• [54]TOMMASINI L,YAHIAOUI N,SRICHUMPA P,KELLER B.De-velopment of functional markers specific for seven Pm3 resistancealleles and their validation in the bread wheat gene pool[J].Theoreti-cal and Applied Genetics,2006,114(1):165-175.
• [55]CELTON J M,TUSTIN D S,CHAGNE D,GARDINER S E.Con-struction of a dense genetic linkage map for apple rootstocks usingSSRs developed from Malus ESTs and Pyrus genomic sequences[J].Tree Genetics&Genomes,2009,5:93-107.
• [56]COLLARD C Y,MACKILL D J.Start codon targeted(SCoT)poly-morphism:a simple,novel DNA marker technique for generatinggene-targeted markers in plants[J].Plant Mol Biol Rep,2009,27(1):86-93.
• [57]GULSEN O,UZUN A,CANAN I,SEDAY U,CANIHOS E.A newcitrus linkage map based on SRAP,SSR,ISSR,POGP,RGA andRAPD markers[J].Euphytica,2010,173:265-277.
• [58]YAMAMOTO T,KIMURA T,TERAKAMI S,NISHITANI C,SAWAMURA Y,SAITO T,KOTOBUKI K,HAYASHI T.Integrat-ed reference genetic linkage maps of pear based on SSR and AFLPmarkers[J].Breeding Science,2007,57:321-329.
• [59]QIAO Yan-chun.Construction of molecular genetic linkage map andgenetic diversity of genus Eriobotrya[D].Guangzhou:South China A-gricultural University,2008.乔燕春.枇杷属植物分子遗传图谱的构建及遗传多样性研究[D].广州:华南农业大学,2008.
• [60]PAN Xin-fa,MENG Xiang-xun,CAO Guang-li,XU Chun-ming.Application of RAPD-PCR technique for identification of varietiesin loquat[J].Journal of Fruit Science,2002,19(2):136-138.潘新法,孟祥勋,曹广力,徐春明.RAPD在枇杷品种鉴定中的应用[J].果树学报,2002,19(2):136-138.
• [61]SORIANO J M,ROMERO C,VILANOVA S,LL魣CER G,BADENESM L.Genetic diversity of loquat germplasm(Eriobotrya japonica(Thunb.)Lindl.)assessed by SSR markers[J].Genome,2005,48(1):108-113.
• [62]LU Cai-rui,YU Shu-xun,YU Ji-Wen,FAN Shu-li,SONG Mei-zhen,WANG Wu,MA Shu-juan.Development and appraisement offunctional molecular marker:intron sequence amplified polymor-phism(ISAP)[J].Hereditas,2008,30(9):1207-1216.陆才瑞,喻树迅,于霁雯,范术丽,宋美珍,王武,马淑娟.功能型分子标记(ISAP)的开发及评价[J].遗传,2008,30(9):1207-1216.
• [63]LIU Z H,ANDERSON J A,HU J,FRIESEN T L,RASMUSSEN JB,FARIS J D.A wheat intervarietal genetic linkage map based onmicrosatellite and target region amplified polymorphism markers andits utility for detecting quantitative trait loci[J].Theoretical andApplied Genetics,2005,111(4):782-794.
• [64]IYER-PASCUZZI A S,MCCOUCH S R.Functional markers forxa5-mediated resistance in rice(Oryza sativa,L.)[J].Molecular Breed-ing,2007,19:291-296.
• [65]SONG Wei,WANG Feng-ge,YI Hong-mei,LI Xiang,ZHAO Jiu-ran.Functional markers and their potential application in varieties i-dentification and MAS breeding[J].Molecular Plant Breeding,2009,7(3):612-618.宋伟,王凤格,易红梅,李翔,赵久然.功能标记及在品种鉴定和辅助育种中的应用前景[J].分子植物育种,2009,7(3):612-618.
• [66]WANG Yun-sheng,LIN Shun-quan.A comparative study on thepolymorphism of loci cpDNATrnS-TrnG and cpDNATrnQ-rps16 in cultivatedand wild loquats[J].Acta Horticulturae Sinica,2012,39(10):1913-1918.王云生,林顺权.枇杷野生型与栽培型的cpDNATrnS-TrnG及cpDNATrnQ-rps16序列多态性的比较[J].园艺学报,2012,39(10):1913-1918.