黑穗醋栗组织培养和遗传转化Study of tissue culture and genetic transformation on blackcurrant
徐洪国;高庆玉;祁宏英;
摘要(Abstract)：
通过对不同激素质量浓度配比实验,建立黑穗醋栗茎尖高频再生体系。结果表明,MS+6-BA1.0mg·L-1+NAA0.1mg·L-1培养基可高效诱导黑穗醋栗茎尖的分化,1/2MS培养基可快速诱导根的生成,形成再生植株。25mg·L-1卡那霉素可以抑制茎尖的分化,20mg·L-1卡那霉素可以抑制分化小苗的生根。利用农杆菌介导法将osMAPK4基因转化黑穗醋栗,获得了抗卡那霉素的再生植株。提取转基因植株基因组DNA,进行PCR检测,结果表明osMAPK4基因已经被整合到黑穗醋栗基因组中。
关键词(KeyWords)： 黑穗醋栗;组织培养;遗传转化
基金项目(Foundation): 国家行业科技基金项目(编号:nyhyzx07-028)
作者(Author): 徐洪国;高庆玉;祁宏英;
Email:
DOI:
参考文献(References)：
- [1]ZENG Li-hui,LV Liu-xin.Advances in research on genetic transfor-mation in woody fruit cr-ops[J].Journal of Fruit Science,2002,19(3):191-198.曾黎辉,吕柳新.木本果树遗传转化研究进展[J].果树学报,2002,19(3):191-198.
- [2]FANG Hong-jun,WANG Guan-lin,WANG Huo-xu,JIA Shi-rong,DONG Yun-zhou,TANG Yi-xiong.Pathog-enresistant transgenic plant from dwarfing r-ootstock of cherry by introducing antibacter-ial polypeptide Genes[J].Acta Botanica Sinica,1999,41(11):1192-1198.方宏筠,王关林,王火旭,贾士荣,董云洲,唐益雄.抗菌肽基因转化樱桃矮化砧木获得抗根瘤病的转基因植株[J].植物学报,1999,14(11):1192-1198.
- [3]SHI Xiao-xin,WANG Bin,DU Guo-qiang,WENG Man-li,GAO Yi.Studies on agrobacterium mediated cp-ti gene tranfer in commercial apple caltivar[J].Acta Horticulturae Sinica,2000,27(4):282-284.师校欣,王斌,杜国强,翁曼丽,高仪.根癌农杆菌介导豇豆胰蛋白酶抑制剂基因转入苹果主栽品种[J].园艺学报,2000,27(4):282-284.
- [4]P.GUTIERREZ-PESCE,K.TAYLOR,R.MULEO,E.RUGINI.Somatic embryogenesis and shoot reg-eneration from transgenic roots of the cherry rootst-ock Colt mediated by pRi1855T-DNA of A-grobac-terium rhizogenes[J].Plant Cell Rep,1998,17:574-580.
- [5]DOMINGUEZ A,GUERRI J,CAMBRA M,NAVARRO L,MORENO P,PENA L.Efficient production of transgenic citrus plants express-ing the coat pro-tein of citrus tristeza virus[J].Plant Cell Rep,2000,19(04):427-437.
- [6]PENA L,PEREZ R M,CERVERA M,JUAREZ J A,LUIS NAVAR-RO.Early events in agrobacterium-mediated genetic transformation of citrus expl-ants[J].Annals of Botany,2004,94:67-74.
- [7]BONDT A D,EGGERMONT K,PENNINCKX I,GODERIS I,BROEKAERT W E.Agrobacterium mediated transformation of apple(Malus×domestica Borkh.):an assessment of factors affe cting re-generation oftransgenic plants[J].Plant Cell Rep,1996(15):549-554.
- [8]YANCHEVA S D,SHLIZERMAN L A,GOLUBOWIEZ S,YABLVIZ Z,PERL A,HANANIA U,FLAISHMAN M A.The use of green fluo-rescent protein(GFP)improves agrobacterium mediated transfor-mation of Spadona pear(Pyrus comm unis L.)[J].Plant Cell Rep,2006(25):183-189.
- [9]MIGUEL C M,OLIVEIRA M M.Transgenic almond(Prunus dulcis Mill.)plants obtained by Agrobacterium-mediated transformation of leaf explants[J].Plant Cell Rep,1999(18):387-393.
- [10]KRASTANOVA S,PERRIN M,BARBIER P,DEMANGEAT G,CORNUET P,BARDONNET N,OTTEN L,PINCK L,WALTER B.Transformation of grapevine rootstocks with the coat protein gene of grapevine fanleaf nepovirus[J].Plant Cell Reports1995,14:550-554.
- [11]TONG Zhao-guo,CAI Bin-hua,ZHANG Zhen,WANG Fu-rong,ZHANG Kai-chun,YAN Guo-hua,ZHOU Yu.Advances in applica-tion research on genetic transformation of fruit trees via par ticle bombardment[J].Journal of Fruit Science,2008,25(3):389-395.佟兆国,蔡斌华,章镇,王富荣,张开春,闫国华,周宇.果树中基因枪法遗传转化的研究进展[J].果树学报,2008,25(3):389-395.
- [12]VOISEY C R,WHITE D W R,DUDAS B,APPLEBY R D,Agrob acterium-mediated transformation of white clover using direct shoot organogenesis[J].Plant Cell Repo rts,1994(13):309-314.