银杏成熟胚乳培养的细胞组织学观察In vitro Culture of the Mature Endosperm of Ginkgo biloba L.and Cytohistological Studies
吴元立,严学成
摘要(Abstract):
诱导银杏(GinkgobilobaL.)成熟胚乳产生愈伤组织时无需胚的存在。单独使用生长素或在细胞分裂素/生长素为0.5~0.9的范围内均能诱导部分胚乳切块产生愈伤组织。并且在White+Kt2.7mg·l-1+2,4-D1.0mg·l-1+NAA2.0mg·l-1的培养基上,愈伤组织的诱导率最高,达到83.3%。胚乳细胞脱分化进行有丝分裂和无丝分裂,其中无丝分裂可以观察到劈裂和缢裂两种类型。愈伤组织起源于胚乳表皮及其下方2~3层细胞,且愈伤组织的形成需经历一个“假愈伤组织化”阶段。
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作者(Author): 吴元立,严学成
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DOI: 10.13925/j.cnki.gsxb.1998.04.009
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