HPLC法测定番木瓜种子对羟基苯甲酸和香草酸的含量(英文)Determination of p-hydroxybenzoic acid and vanillic acid in papaya seed extracts using HPLC
周开兵;王辉;梅文莉;李小娜;罗应;戴好富;
摘要(Abstract)：
【目的】为了测定番木瓜种子醇、石油醚、乙酸乙酯、正丁醇和水提取物中的对羟基苯甲酸和香草酸含量,【方法】建立了测定各提取物中的2种酚酸含量的HPLC法,并检测了2种酚酸的含量。【结果】标样浓度介于1～5 mg.L-1时,标样浓度与峰面积间具有良好线性回归关系;本试验所优化的HPLC法具备良好的精密性、重演性和稳定性,简便易行,适于检测番木瓜种子粗提物中的对羟基苯甲酸和香草酸含量;2种酚酸含量以乙酸乙酯提取物中最高、正丁醇提取物中次之,水提取物中则几乎未能检测到。【结论】2种酚酸主要存在于乙酸乙酯和正丁醇提取物中,在水提取物中几乎不存在。
关键词(KeyWords)： 番木瓜;种子;对羟基苯甲酸;香草酸;HPLC
基金项目(Foundation): 海口市重点科技项目(2010082);; 中国热带农业科学院重点科技项目
作者(Authors): 周开兵;王辉;梅文莉;李小娜;罗应;戴好富;
DOI: 10.13925/j.cnki.gsxb.2012.05.038
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