宋盼;赵凤莉;宋艳红;赵倩倩;李刚;赵霞;刘丽锋;周厚成;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

【目的】分析不同颜色草莓果实中花色苷合成相关结构基因与FaMYB10的表达差异,以及FaMYB10转录调控的功能验证。【方法】以栽培草莓‘红颜‘’小白’和‘白雪公主’为试材,利用RNA-seq技术筛选花色苷合成差异表达基因并进行qRT-PCR验证,利用同源重组法构建35S::FaMYB10过表达载体,通过离体果实瞬时转化验证其在果实花色苷合成调控中的作用。【结果】从3个品种不同时期果实转录组数据中筛选到8个参与果实花色苷合成的候选基因,包括FaPAL、FaCHI、FaCHS、FaDFR、FaANS、FaUFGT、Fa3GT和FaGST,这8个候选基因在‘红颜’和‘小白’成熟过程中表达量逐渐升高,且均显著高于‘白雪公主’,基因表达模式与3个品种果实花色苷的合成积累模式相一致。qRT-PCR结果表明,果实发育成熟期FaMYB10在‘白雪公主’和‘小白’果实中的表达量均显著高于‘红颜’,其表达模式与转录水平变化一致。‘小白’FaMYB10-XB基因编码区序列存在2种类型,其中FaMYB10-XB-2插入序列GA‘;白雪公主’FaMYB10-SW编码区序列具有ACTTATAC插入。果实瞬时过表达结果证明FaMYB10可增加花色苷的合成和积累。【结论】不同表型果实在各个时期,果实总花色苷含量水平有显著差异;8个候选基因中,FaCHI、FaDFR、FaANS和FaGST在果实发育成熟中,白果类型的表达水平显著低于红果类型,推测其可能是参与果实花色苷合成途径的关键结构基因;然而,白果类型FaMYB10的表达水平却显著高于红果类型;果实瞬时转化证明FaMYB10是不同颜色果实花色苷合成的关键正调控因子,但FaMYB10序列突变使其调控功能丧失,可能是白果类型花色苷积累缺失的原因。

关键词(KeyWords)： 草莓;花色苷合成;结构基因;转录因子;基因表达

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所基本科研业务费专项(1610192020601);; 中国农业科学院科技创新工程(CAAS-ASTIP-2020-ZFRI,CAAS-XTCX20190025-4)

作者(Author): 宋盼;赵凤莉;宋艳红;赵倩倩;李刚;赵霞;刘丽锋;周厚成;

Email:

DOI: 10.13925/j.cnki.gsxb.20200121

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