红肉猕猴桃花色苷组成及浸提研究Indentification and extraction of anthocyanins in red-fleshed kiwifruit
刘颖;赵长竹;吴丰魁;陈锦永;刘杰超;顾红;杨晓红;方金豹;
摘要(Abstract)：
以全红河南猕猴桃(Actinidia arguta var.henanensis)为试材,利用高效液相色谱(HPLC)和高效液相色谱-三重四级杆质谱联用技术(HPLC-MS-MS)定性定量分析其花色苷组分,并运用正交试验优化通常情况下决定花色苷提取效果的甲酸强度、料液比、超声时间等3个重要工艺参数。结果表明,红肉猕猴桃果实中有5种花色苷,分别是未知矢车菊素(Cy.)、矢车菊素-3-O-木糖(1-2)-半乳糖苷(Cy-xyl.gal.)、矢车菊素-3-O-半乳糖苷(Cy-gal.)、飞燕草-3-O-木糖(1-2)-半乳糖苷(Dp-xyl.gal.)和飞燕草-3-O-半乳糖苷(Dp-gal.)。每种花色苷浸提值达到最高时对应的甲酸、料液比、超声时间不同。
关键词(KeyWords)： 河南猕猴桃;红色素;花色苷;鉴定;浸提
基金项目(Foundation): 中国农业科学院果树生长发育与品质控制重点开放实验室项目(PP333KF20100102);; 河南省科技攻关项目(102102110045)
作者(Author): 刘颖;赵长竹;吴丰魁;陈锦永;刘杰超;顾红;杨晓红;方金豹;
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