苹果抗斑点落叶病突变体的细胞筛选In vitro Screening of Apple Mutant Resistant to Apple Alternaria Blotch (Alternaria mali Roberts)
彭明生,郭兆年
摘要(Abstract)：
从苹果斑点落叶病菌(Alternaria mali Roberts)的Richards培养液中提取的AM—毒素,在254nm处有吸收峰。经叶片法、插枝法、电导率法等生物测试表明,AM—毒素具有寄主专化性。以AM—毒素为选择因子,配制成选择培养基,以感病品种岩富10、新乔纳金、国光、新红星的愈伤组织为材料,采用多步选择系统筛选抗病突变体。所得到的抗病系离开选择因子2个月后,仍保持获得的抗病性。与未经选择的原始型相比,抗病系的苯丙氨酸解氨酶活性增强,过氧化物酶同工酶带减少1～3条。在AM—毒素作用下,抗病系电导率增加速度显著小于原始型。接种病原菌孢子后,抗病系较原始型抗病。
关键词(KeyWords)： 苹果;AM—毒素;愈伤组织;抗病突变体;细胞筛选
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作者(Author): 彭明生,郭兆年
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