付晴晴;谭雅中;翟衡;杜远鹏;

• 1:山东农业大学园艺科学与工程学院·作物生物学国家重点实验室

摘要(Abstract)：

【目的】探究100 mmol·L^(-1)NaCl胁迫下,耐盐性不同的葡萄株系矿质离子运输和分配的特征,揭示盐胁迫下耐盐性不同的葡萄株系的耐盐机制。【方法】以‘左山一’×SO4杂种砧木F1代的2个株系(A17、A38)以及砧木1103P的1 a(年)生盆栽扦插苗为试材,进行100 mmol·L(-1)NaCl胁迫下,耐盐性不同的葡萄株系矿质离子运输和分配的特征,揭示盐胁迫下耐盐性不同的葡萄株系的耐盐机制。【方法】以‘左山一’×SO4杂种砧木F1代的2个株系(A17、A38)以及砧木1103P的1 a(年)生盆栽扦插苗为试材,进行100 mmol·L^(-1)NaCl胁迫处理,处理20 d后测定各株系根、茎和叶中的离子含量;以A17、A38和1103P继代30 d的组培苗为试材,用100 mmol·L(-1)NaCl胁迫处理,处理20 d后测定各株系根、茎和叶中的离子含量;以A17、A38和1103P继代30 d的组培苗为试材,用100 mmol·L^(-1)NaCl胁迫处理24 h后,利用多通道植物离子检测系统(Younger USA)进行根系Na(-1)NaCl胁迫处理24 h后,利用多通道植物离子检测系统(Younger USA)进行根系Na⁺流变化的检测。【结果】(1)盐胁迫处理下,砧木株系A17叶片及整株中Na+流变化的检测。【结果】(1)盐胁迫处理下,砧木株系A17叶片及整株中Na⁺积累较少,胁迫后的Na+积累较少,胁迫后的Na⁺总量分别是对照的4倍和2.24倍,其根系Na+总量分别是对照的4倍和2.24倍,其根系Na⁺平均净流量为1 513 pmol·cm-2·s-1;而1103P胁迫后的叶片和整株的Na+平均净流量为1 513 pmol·cm-2·s-1;而1103P胁迫后的叶片和整株的Na⁺总量较高,是对照的14.65倍和2.86倍,其根系外排Na+总量较高,是对照的14.65倍和2.86倍,其根系外排Na⁺的平均净流量较低,为863 pmol·cm-2·s-1。(2)A17和A38株系叶片中能够保持较高的K+的平均净流量较低,为863 pmol·cm-2·s-1。(2)A17和A38株系叶片中能够保持较高的K⁺、Ca+、Ca⁽²⁺⁾、Mg(2+)、Mg⁽²⁺⁾和Fe(2+)和Fe⁽²⁺⁾总量以及K(2+)总量以及K⁺/Na+/Na⁺、Ca+、Ca⁽²⁺⁾/Na(2+)/Na⁺和Mg+和Mg⁽²⁺⁾/Na(2+)/Na⁺比值,维持相对较高的SK,Na、SCa,Na和SMg,Na值。【结论】耐盐性强的砧木株系吸收钠离子含量少,外排Na+比值,维持相对较高的SK,Na、SCa,Na和SMg,Na值。【结论】耐盐性强的砧木株系吸收钠离子含量少,外排Na⁺的能力较强,且根系具有较强的Na+的能力较强,且根系具有较强的Na⁺截留作用,并且保持叶片内K+截留作用,并且保持叶片内K⁺、Ca+、Ca⁽²⁺⁾、Mg(2+)、Mg⁽²⁺⁾和Fe(2+)和Fe⁽²⁺⁾等离子含量较高,维持植株内部的离子平衡。

关键词(KeyWords)： 葡萄砧木;盐胁迫;矿质离子;运输;分配

Abstract:

Keywords:

基金项目(Foundation): 国家葡萄产业技术体系(CARS-30);; 教育部“长江学者和创新团队发展计划”创新团队(IRT15R42);; 山东省公益性重点研发计划(2017GNC13112)

作者(Authors): 付晴晴;谭雅中;翟衡;杜远鹏;

DOI: 10.13925/j.cnki.gsxb.20170188

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