艾呈祥;秦志华;王洁;王长君;安淼;

• 1:山东省果树研究所

摘要(Abstract)：

【目的】从分子水平了解山东牛心柿群体的遗传结构和亲缘关系。【方法】采用SSR分子标记技术对山东省内的8个牛心柿群体共207个个体进行检测,应用POPGENE 1.31软件进行遗传参数分析,采用AREQUIN 2.0软件对群体间和群体内的遗传变异进行分子变异分析,利用TFPGA软件对Nei’s无偏差预期杂合度和基因分化系数进行了统计计算。【结果】10对SSR引物共检测到128个位点,其中105个位点为多态位点,占82%。Nei’s遗传多样性指数(He)在0.153 8~0.247 6,Shannon信息指数(H0)在0.221 7~0.372 1。群体间的遗传变异占总遗传变异的24.17%(P<0.001),群体间的遗传距离(D<0.231 7)、遗传一致度(I>0.783 2)、基因分化系数(φST=0.222 6)和遗传分化系数(FST=0.175 9)均表明各群体间产生了一定程度的遗传分化。【结论】山东牛心柿群体遗传背景丰富,群体间一定程度的遗传分化可能是人为选择压力所引起。

关键词(KeyWords)： 牛心柿;SSR;遗传多样性;聚类分析

Abstract:

Keywords:

基金项目(Foundation): “十二五”农村领域国家科技计划项目(2013BAD14B05);; 山东省农业良种工程(2010lz010)

作者(Author): 艾呈祥;秦志华;王洁;王长君;安淼;

Email:

DOI: 10.13925/j.cnki.gsxb.2013.04.015

参考文献(References)：

• [1]LUO Z R,WANG R Z.Persimmon in China:Domestication and tra-ditional utilizations of genetic resources[J].Advances in Horticultural Science,2008,22(4):239-243.
• [2]YUAN Lu-xia,ZHANG Qing-lin,GUO Da-yong,LUO Zheng-rong.Characteristics of Chinese PCNA typesand their roles in sci-ence and industry of oriental persimmon[J].Acta Horticulturae Sini-ca,2011,38(2):361-370.袁录霞,张青林,郭大勇,罗正荣.中国甜柿及其在世界甜柿基因库中的地位[J].园艺学报,2011,38(2):361-370.
• [3]LIU Zhen-yan,LI Zhen-san.Shandong fruits[M].Shanghai:Shanghai Scientific and Technical Publishers,2000:861-864.刘振岩,李震三.山东果树[M].上海:上海科学技术出版社,2000:861-864.
• [4]WU Hong-fen,WANG Tong-xiang,WANG Wei.Cultivation of ori-ental persimmon(Diospyros kaki Thunb.)[J].Deciduous Fruits,2007,39(1):37-38.吴洪芬,王同祥,王卫.牛心柿的栽培技术[J].落叶果树,2007,39(1):37-38.
• [5]CAI Cong-li,WANG Jian-bo,JING Run-chun,ZHU Guo-ying.RAPD analysis of the genome evolution in allopolyploid in Aegilops[J].Acta Genetica Sinica,2001,28(2):158-165.蔡从利,王建波,景润春,朱国英.山羊草属异源多倍体植物基因组进化的RAPD分析[J].遗传学报,2001,28(2):158-165.
• [6]WANG Zhuo-wei,YU Mao-de,LU Cheng,YANG Guang-wei,JING Cheng-jun.AFLP analysis of genetic background of polyploidy breeding[J].Caoye Kexue,2001,27(3):170-176.王卓伟,余茂德,鲁成,杨光伟,敬成俊.桑树多倍体育种材料遗传背景的AFLP分析[J].草业科学,2001,27(3):170-176.
• [7]LIU Ai-hua,WANG Jian-bo,ZHU Ying-guo.RAPD analysis on the genome evolution of polyploids in Brassica[J].Acta Phytotaxo-nomica Sinica,2003,41(6):520-530.刘爱华,王建波,朱英国.芸薹属多倍体植物基因组进化的RAPD分析[J].植物分类学报,2003,41(6):520-530.
• [8]LU Yun-cai,WANG Hua-zhong.Genetic analysis of sugarbeet poly-ploid varieties by RAPD markers[J].Sugar Crops of China,2006(3):5-8.路云才,王华忠.我国甜菜多倍体品种的RAPD分析[J].中国糖料,2006(3):5-8.
• [9]DOYLE J J,DOYLE J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochem Bulletin,1987,19:11-15.
• [10]SORIANO J M,PECCHIOLI S,ROMERO C.Development of mi-crosatellite markers in polyploidy persimmon(Diodpyros kaki Lf)from an enriched genomic library[J].Molecular Ecology Notes,2006,6:368-370.
• [11]NAVAL M d M,ZURIAGA E,PECCHIOLI S,LIACER G,GIOR-DANI E,BADENES M L.Analysis of genetic diversity among per-simmon cultivars using microsatellite markers[J].Tree Genetics&Genomes,2010,6:677-687.
• [12]GUO D L,LUO Z R.Microsatellite isolation and characterization in Japanese persimmon(Diospyros kaki)[J].Biochem Genet,2008,46:323-328.
• [13]NEI M.Analysis of gene diversity in subdivided populations[J].Pro-ceeding of the National Academy of Sciences,USA,1973,70:3321-3323.
• [14]NEI M.Estimation of average heterozygosity and genetic distance from a small number of individuals[J].Genetics,1978,89:583-590.
• [15]WEIR B S,COCKERHAM C C.Estimating F-statistics for the anal-ysis of population structure[J].Evolution,1984,38:1358-1370.
• [16]HAMRICK J L.Isozyme and analysis of genetic structure in plant population[M]//In:Solits D E,Solits P S,eds Isozymes in plants bi-ology.Dioscorides Press,Portland,Ore,1989:87-105.
• [17]ESTOUP A,ANGES B.Microsatellites and minisatellites for molec-ular ecology:theoretical and empirical considerations[M]//In:Car-valho,G.R.(ed.),Advances in Molecular Ecology.IOS Press,Am-sterdam,1998:55-86.
• [18]SLATKIN M.Estimating levels of gene flow in natural populations[J].Genetics,1981,99:323-335.