侯晓婉;胡伟;徐碧玉;张鲁斌;金志强;

• 1:中国热带农业科学院南亚热带作物研究所·海南省热带园艺产品采后生理与保鲜重点实验室
• 2:中国热带农业科学院热带生物技术研究所·农业部热带作物生物学与遗传资源利用重点实验室

摘要(Abstract)：

【目的】筛选、克隆响应非生物胁迫的关键ERF转录因子基因,并研究其功能。【方法】综合运用转录组学、生物信息学、生物化学等技术手段,分析不同非生物逆境胁迫下AP2/ERF转录因子超家族基因的表达谱聚类变化,并从中筛选、克隆关键ERF转录因子,对其功能进行研究。【结果】不同非生物胁迫下,AP2/ERF超家族基因的表达模式不同,对低温胁迫的响应较其他胁迫敏感;筛选克隆的Ma ERF25和Ma ERF27基因是ERF家族基因,具备ERF家族基本特征,编码蛋白为核定位蛋白,C端具有转录激活活力,并参与了香蕉对非生物胁迫和激素的应答,在其中发挥着一定的作用。【结论】获得了2个巴西蕉ERF基因Ma ERF25和Ma ERF27,被定位在细胞核,具有转录激活活性,参与非生物逆境胁迫应答。

关键词(KeyWords)： 香蕉;AP2/ERF;基因克隆;亚细胞定位;转录激活;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系(国家香蕉产业技术体系)建设专项(CARS-32);; 973计划前期研究专项(2014CB160314);; 香蕉、杧果、菠萝保鲜、贮运关键技术集成与产业化应用(ZDZX2013011)

作者(Author): 侯晓婉;胡伟;徐碧玉;张鲁斌;金志强;

Email:

DOI: 10.13925/j.cnki.gsxb.20160436

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