王苏珂;杨健;王龙;周厚成;李秀根;

• 1:中国农业科学院郑州果树研究所

摘要(Abstract)：

以西洋梨矮化砧木BA-29春季茎段为外植体,筛选适宜的消毒时间和诱导、继代增殖与生根培养基,并对试管苗进行了移栽。结果表明,(1)取春季田间嫩茎段为外植体,用0.1%HgCl2消毒5min,污染率可控制在20%以下,外植体成活率超过70%,效果优于消毒3min、7min和10min;以1/2MS+6-BA1mg·L-1+IBA0.1mg·L-1+GA0.2mg·L-1为诱导培养基,增殖倍数较高;(2)适宜继代增殖培养基为MS+6-BA1mg·L-1+IBA0.4mg·L-1+GA0.05mg·L-1,增殖系数可以达到3.8,且芽生长势强,新芽质量好;(3)适宜生根培养基为1/2MS+NAA0.5mg·L-1+IBA0.5mg·L-1,生根率81%,平均生根7.6条,最多达到10条;已生根试管苗炼苗7d后移栽于灭过菌的基质中,30d后移栽成活率超过80%。

关键词(KeyWords)： 西洋梨;矮化砧木;BA-29;组织培养;快速繁殖

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(nyhyzx07-026);; 国家现代农业产业(梨)技术体系(nycytx-29);; 农业部‘948’重点项目(2009-G27)

作者(Author): 王苏珂;杨健;王龙;周厚成;李秀根;

Email:

DOI: 10.13925/j.cnki.gsxb.2010.06.007

参考文献(References)：

• [1]WANG Cai-hong,TIAN Yi-ke,DAI Hong-yi,DAI Qing-hai,YIN Zhao-xue,YANG Xiao-qin.Study of the biological characters of a dwarf pear resource from Pyrus communis[J].Journal of Laiyang A-gricultural College:Natural Science Edition,2006,23(2):119-121.王彩虹,田义轲,戴洪义,代庆海,殷召学,杨晓芹.西洋梨矮化资源的生物学特性研究[J].莱阳农学院学报:自然科学版,2006,23(2):119-121.
• [2]JI Hua,ZHANG Hai-xin,ZHAO Yu-fen,GE Hui-bo.Influencing factors on multiplication coefficient and regeneration quality of ad-ventitious shoots of the S series dwarfing rootstock of pear[J].Journal of Hebei Agricultural Sciences,2004,8(2):40-44.及华,张海新,赵玉芬,葛会波.梨S系矮化砧组培苗增殖系数和质量的影响因子[J].河北农业科学,2004,8(2):40-44.
• [3]DOLCET-SANJUAN R,DAVID W S.Mok,MACHTELD C.Mok.Plantlet regeneration from cultured leaves of cydonia oblonga L.(quince)[J].Plant Cell Reports,1991,10:240-242.
• [4]MARINO G,BERARDI G.Different sealing materials for petri dish-es strongly affect shoot regeneration and development from leaf ex-plants of quince“BA29”[J].In vitro Cellular Developmental Biology Plant,2004,40(4):384-388.
• [5]XU Jian-lan,MA Rui-juan,YU Ming-liang,SHEN Zhi-jun.Studies on the tissue culture of Damas1869plum[J].Journal of Fruit Sci-ence,2008,25(5):740-743.许建兰,马瑞娟,俞明亮,沈志军.Damas1869李组培繁殖技术研究[J].果树学报,2008,25(5):740-743.
• [6]LIU Jie,ZHANG Yu–xing,DONG Zhen.Progress on pear’s explants browning and anti-browning measuresin the process of tissue cul-ture[J].Hebei Journal of Forestry and Orchard Resrarch,2008,23(2):195-199.刘杰,张玉星,董祯.梨组培褐化及抗褐措施研究进展[J].河北林果研究,2008,23(2):195-199.
• [7]HUANG Jing-hao,XIE Jiang-hui,WANG Song-biao,LIANG Guo-lu,MA Wei-hong.Studies on somatic embryogenesis and plant re-generation of Mangifera persiciformis[J].Journal of Fruit Science,2009,26(2):176-179.黄镜浩,谢江辉,王松标,梁国鲁,马蔚红.扁桃杧(Mangiferaper-siciformis)体胚发生及再生体系建立[J].果树学报,2009,26(2):176-179.
• [8]JI Hua,ZHANG Hai-xin,GE Hui-bo.The relationship between browning ratio in PPO and phenols of pear explants[J].Plant Physi-ology Communications,2006,42(1):63.及华,张海新,葛会波.梨组织培养过程中外植体褐变与多酚氧化酶及酚类物质的关系[J].植物生理学通讯,2006,42(1):63.
• [9]XU Xiao-yan,SUN Wu-san,WANG Neng-ru.Synthesis of polyphe-nolic compounds and their effects on tobacco quality[J].Chinese To-bacco Science,2003(1):3-5.徐晓燕,孙五三,王能如.烟草多酚类化合物的合成与烟叶品质的关系[J].中国烟草科学,2003(1):3-5.
• [10]LIU Jie,ZHANG Xi,POUDYAL Bharat Kumar,ZHANG Yu-xing,DONG Zhen,SHI Qing-chun.Studies on factors affecting browning of pear explants in vitro and anti-browning measures[J].Journal of Fruit Science,2008,25(5):727-731.刘杰,张希,POUDYAL Bharat Kumar,张玉星,董祯,史青纯.梨外植体组培褐变的影响因子及预防措施[J].果树学报,2008,25(5):727-731.
• [11]TANG Shao-hu,SUN Min,ZHOU Qi-gui,LONG Yun,LI Dao-gao.Establishment of high frequently regeneration system from leaves of Xueqing Pear[J].Acta Horticulturae Sinica,2005,32(6):1084-1087.汤绍虎,孙敏,周启贵,龙云,李道高.雪青梨叶片高频再生体系的建立[J].园艺学报,2005,32(6):1084-1087.
• [12]JIN Qing,CAI Yong-ping.Tissue culture and rapid propagation of Pyrus bretschneideri Rehd[J].Plant Physiology Communications,2006,42(5):900.金青,蔡永萍.砀山酥梨的组织培养和脱毒快速繁殖[J].植物生理学通讯,2006,42(5):900.