张平贤;张娜;郑洁;黄金盟;张青林;徐莉清;罗正荣;

• 1:华中农业大学园艺植物生物学教育部重点实验室
• 2:广西特色作物研究院
• 3:大别山特色资源开发湖北省协同创新中心

摘要(Abstract)：

【目的】绝大多数柿品种仅开雌花,但中国甜柿‘鄂柿1号’可偶尔开雄花,探讨该现象与DNA变异有无关系及其育种学意义。【方法】利用荧光AFLP技术探讨‘鄂柿1号’雄花枝梢与其母株间的基因组是否存在变异;通过检测其花粉生物学特性以及F1是否存在与中国甜柿自然脱涩连锁的RO2标记和与雄性性别紧密连锁的Dl Sx-AF4S标记,探讨其作为中国甜柿育种亲本的可能性。【结果】(1)AFLP分析表明已知遗传关系的‘前川次郎’和‘次郎’(前者为后者的早熟芽变类型)的相似系数0.88,而‘鄂柿1号’雄枝与母株的相似系数为1.00,说明‘鄂柿1号’偶开雄花性状可能与DNA序列变异无关;(2)‘鄂柿1号’雄枝花粉离体萌发率较低,但其与‘太秋’和‘早秋’柱头亲和性较强;(3)‘鄂柿1号’作父本与‘太秋’和‘早秋’杂交,以及作母本与‘太秋’杂交,均可获得携带RO2标记的F1代单株,且在其作父本的部分F1代单株中检测不到Dl Sx-AF4S标记。【结论】‘鄂柿1号’偶开雄花现象可能不涉及遗传物质DNA序列的改变,其变异原因有待进一步探讨;其作杂交父本时F1单株中均携带与中国甜柿自然脱涩性状连锁的RO2标记,且部分单株不含与雄性性状连锁的Dl Sx-AF4S标记,说明其在选育仅开雌花的完全甜柿新品种中具应用潜力。

关键词(KeyWords)： 柿;‘鄂柿1号’;偶开雄花;遗传变异;育种价值

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(201203047);; 大别山特色资源开发湖北省协同创新中心项目(2015TD01);; 国家科技基础性工作专项(2012FY110100)

作者(Authors): 张平贤;张娜;郑洁;黄金盟;张青林;徐莉清;罗正荣;

DOI: 10.13925/j.cnki.gsxb.20160308

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