汪开拓;雷长毅;黎春红;邱玲岚;匡文玲;

• 1:重庆三峡学院生命科学与工程学院
• 2:南京农业大学食品科技学院

摘要(Abstract)：

【目的】从转录组学角度探讨经β-氨基丁酸(β-aminobutyric acid,BABA)处理的草莓果实对病害胁迫的响应及其与细胞还原势变化的联系,以阐释其中诱导抗性的形成机制。【方法】‘丰香’草莓果实经10 mmol·L-1BABA及病原菌Botrytis cinerea接种后于(20±1)℃下贮藏5 d,期间每隔1 d测定果实发病率及还原性物质合成量,并以Illumina HiSeqTM2500技术对果实样本进行转录组测序。【结果】BABA+B. cinerea处理组较单一BABA处理和病原菌接种可更为显著诱导草莓果实PRs基因丰度的上升,并抑制果实灰霉病发生和维持品质。对差异表达基因(DEGs)的GO和KEGG聚类分析显示,经BABA处理后再接种B. cinerea的草莓果实中抗坏血酸-谷胱甘肽循环(AsA-GSH循环)关键基因、活性氧代谢酶系统关键基因、谷胱甘肽(GSH)合成途径关键基因、磷酸戊糖途径(PPP)关键基因及GSH过氧化物酶转录水平显著提高,同时果实中还原性物质逐渐积累。【结论】BABA可通过提高草莓果实还原性物质合成相关基因表达量,增强果实中还原性底物的合成以提升细胞还原势,促使果实在病原菌侵染时展现priming抗病性,抑制果实采后灰霉病症状的发展。

关键词(KeyWords)： 草莓果实;β-氨基丁酸;转录组;还原势;抗病性诱导;品质

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金面上项目(31671913);; 重庆市第五批高校优秀人才支持计划(201731);; 重庆市基础与前沿研究计划项目(cstc2015jcyj A80028);; 2019年度重庆市大学生创新性实验计划项目(201910643039)

作者(Author): 汪开拓;雷长毅;黎春红;邱玲岚;匡文玲;

Email:

DOI: 10.13925/j.cnki.gsxb.20200162

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