宁文彬;刘菊华;贾彩红;徐碧玉;金志强;

• 1:中国热带农业科学院热带生物技术研究所
• 2:海南大学农学院

摘要(Abstract)：

乙醇脱氢酶(ADH)在果实的生长发育以及果实成熟过程中起到一定的作用。以香蕉果实为材料,测定了乙烯、1-甲基环丙烯(1-MCP)处理对香蕉果实采后乙烯释放量和ADH活性的影响,以了解ADH活性与乙烯代谢的关系。结果表明,与正常后熟的香蕉相比,乙烯处理使香蕉果实的乙烯释放量提前11 d达到峰值,ADH活性提前11 d开始上升。1-MCP处理则显著抑制了香蕉的乙烯释放,没有出现乙烯峰,ADH活性也被抑制,活性变化不明显。推测在香蕉果实采后成熟过程中乙醇脱氢酶(ADH)活性与乙烯代谢成正相关,乙醇脱氢酶(ADH)活性可能受乙稀代谢调控。

关键词(KeyWords)： 香蕉;乙醇脱氢酶;乙烯;1-甲基环丙烯

Abstract:

Keywords:

基金项目(Foundation): 公益性行业(农业)科研专项(nyhyzx07-029);; 热带生物技术研究所中央级公益性科研院所基本科研业务费专项资金(ITB-BZD0733)

作者(Author): 宁文彬;刘菊华;贾彩红;徐碧玉;金志强;

Email:

参考文献(References)：

• [1]JAMES J,GIOVANNONI.Genetic regulation of fruit developmentand ripening[J].The Plant Cell,2004,16:170-180.
• [2]SISLER E C,SEREK M.Inhibitors of ethylene responses in plants atthe receptor level:recent developments[J].Physiol Plant,1999,100(3):577-582.
• [3]JACKSON M B,DREW M C.Flooding and plant growth[J].Academ-ic Press,1984,47:123-128.
• [4]KANELLIS A K,SOLAMOS T,ANGELAK K A.Suppression of cel-lulose and polygalacturonase and induction of alcohol dehydrogenaseisoenzymes in avocado fruit me so carp subjected to low oxygen stress[J].Plant Physiology,1991,96:269-274.
• [5]PINHERO R G,COPP L J,AMAYA C L,MARANGONI A G,YADAR Y.Roles of alcohol dehydrogenase,lactate dehydrogenase andpyruvate decarboxylase in low-tenperature sweetening in to lerantand susceptible varieties of potato(Solanum tuberosum)[J].Physiolo-gia Plantarum,2007,130:230-239.
• [6]TESNIERE C,MARTINE P.Involvement of ethylene signaling inanon-climacteric fruit:new elements regarding the regulation ofADHexpressioningrapevine[J].JournalofExperimentalBotany,2004,406:2235-2240.
• [7]DANIEL MANRIQUEZ,ISLAM E S,FRANCISCO B F.Two highlydivergent alcohol dehydrogenases of melon exhibitfruit ripening-specific expression and distinct biochemical characteristics[J].PlantMol Biol,2006,61:675-685.
• [8]LIU Xue-jun.Characterization of ethylene biosynthesis associatedwith ripening in banana fruit[J].Plant physiology,1999,121:1257-1265.
• [9]SEEMI L,PRABODH K,TRIVEDI.Changes in activities of cell wallhydrolases during ethylene induced ripening in banana:effect of 1-MCP,ABA and IAA[J].Postharvest Biology and Technonlogy,2004,31(2):124-129.
• [10]HANSON A D,JACOSBN J V.Control of lactate dehydrogenase lac-tate glycolysis,and-α-amylase by O2deficitin barley aleurone layers[J].Plant Physiol,1984,75:566-572.
• [11]ZHANG Ming-jing,JIANG Wei-bo,XU Xing-lian.Effects of 1-MCP on postharvest quality of banana fruits[J].Food Science,2002,23(2):126-128.张明晶,姜微波,徐杏连.1-MCP对香蕉果实采后品质的影响[J].食品科学,2002,23(2):126-128.
• [12]PATHAK N,ASTF M,DHAWAN P.Expression and activities ofethylene biosynthesis enzymes during ripening of banana fruits andeffect of 1-MCP treatment[J].Plant Growth Rrgulation,2003,40(1):11-19.
• [13]SCANDALIOS J G.Phytochemical society symposia series No.14regulation of enzyme synthesis and activity in higher plants[J].Aca-demic Press,1977,35:120-135.
• [14]TESNIERE C,VERRIES C.Molecular cloning and expression of cD-NAs encoding alcohol dehydrogenases from Vitis vinifera L.duringberry development[J].Plant Sci,2000,157:77-88.